Correlation between methylation profile and gestational age.

<p>Bisulphite pyrosequencing results for each CpG dinucleotide of the <i>AMOT</i> promoter CpG island. Dots represent methylation values (y-axis) at each CpG for the corresponding gestational age in weeks (x-axis). We used this two variables as quantitative to estimate the relationship. The "cor" value for each plot represents the Pearson correlation coefficient that estimates the link between the two variables and P-values correspond to statistical relevance of the "cor" coefficient. The red lines correspond to the linear regression of the methylation profile as function of gestational age to illustrate the correlation in the plot. A negative value of the cor coefficient corresponds to a deacreasing of the methylation as function of the gestational age for the linear model (decreasing red line). Statistical were performed using R statistical tools.</p

Cloning-sequencing of individual clones of the <i>AMOT</i> promoter CpG island.

<p>Differences in the methylation of <i>AMOT</i> promoter CpG island in newborns are associated with prematurity. (A) Structure of the <i>AMOT</i> CpG proximal promoter island region. Structure of the proximal promoter region of the <i>AMOT</i> gene showing the location of the CpG island: the methylation CpG sites analysed were numbered 1–31. (B) Bisulfite genomic sequencing of individual clones of the <i>AMOT</i> promoter CpG island. Each column represents the methylation status at each CpG dinucleotide in individual clones. Each line represents an individual clone in which the methylation status is determined at each of the 31 CpG dinucleotides. Twenty nine clones from cord blood ECFC of 2 term newborns and forty clones of 3 preterm newborns were examined, respectively. Black and white dots indicate methylated and unmethylated CpGs, respectively. The positions of CpG dinucleotides further analysed by pyrosequencing are indicated by the two lines below dot plots. (C) Sequence of the <i>AMOT</i> promoter CpG island. Pyrosequencing primers are underlined. The nine CpG dinucleotides analysed by pyrosequencing are in bold.</p

Pyrosequencing experiments.

<p>Pyrosequencing experiments.</p

Correlation between methylation profile and gestational age.

<p>Bisulphite pyrosequencing results for each CpG dinucleotide of the <i>AMOT</i> promoter CpG island. Dots represent methylation values (y-axis) at each CpG for the corresponding gestational age in weeks (x-axis). We used this two variables as quantitative to estimate the relationship. The "cor" value for each plot represents the Pearson correlation coefficient that estimates the link between the two variables and P-values correspond to statistical relevance of the "cor" coefficient. The red lines correspond to the linear regression of the methylation profile as function of gestational age to illustrate the correlation in the plot. A negative value of the cor coefficient corresponds to a deacreasing of the methylation as function of the gestational age for the linear model (decreasing red line). Statistical were performed using R statistical tools.</p

Clinical characteristics of patients.

<p>Clinical characteristics of patients.</p

Modulation of <i>AMOT</i> gene expression.

<p>Changes in AMOT expression were assessed by qPCR on 6 cord blood ECFC from preterm neonates or their control counterparts. Each bar represents an individual sample. Each RNA from preterm ECFC was matched against an RNA from a control. Results were normalized to the values obtained with RPL13 expression.</p