Cytoskeleton and major extracellular matrix proteins were analyzed by immunofluorescence.

<p>Actin was observed in DPSC (A), DPSC with BD (B) and in DPSC with BR (C); vimentin and tubulin (D, E and F); and type 1 collagen and fibronectin (G, H and I). The observed decrease in type 1 collagen, when DPSC were in contact with biomaterials, was confirmed using RT-qPCR (5J), whereas protein secretion was not modified by the experimental conditions (Fig 5K). Scale Bar: 100μm.</p

Media used in the study.

<p>Media used in the study.</p

Genotyping results of BRC TgH41001 strain and 12 reference strains with 15 microsatellite markers.

1<p>PTG is a clone of the ME49 strain; CTG is also known as CEP or C strain; COUGAR is also known as TgCgCa1 or COUG strain; BRC TgH18001 is also known as GUY-DOS or GUY-2001-DOS strain; BRC TgH18002 is also known as GUY-KOE or GUY-2002-KOE strain; BRC TgH18003 is also known as GUY-MAT or GUY-2002-MAT.</p

Laboratory findings.

1<p>Not available.</p>2<p>8:00 am measurement.</p>3<p>Vidia Biomérieux.</p>4<p>Vidas Biomérieux.</p

Neighbor-net analysis of genetic diversity on ChrIa.

<p>Neighbor net constructed using sequences from 12 reference blocks (∼800–900 bp each) of ChrIa from representative strains of <i>T. gondii</i>. A strong separation was evident between strains from anthropized and Neotropical rainforest environments of French Guiana. Isolates from anthropized environment harbored a monomorphic version of ChrIa, whereas rainforest isolates contained divergent forms of ChrIa. Color-coding indicates the region of origin and composition of ChrIa: anthropized environment (red), rainforest environment (green), HG reference strains (black), monomorphic ChrIa (yellow), and divergent ChrIa (blue).</p

Virulence and transmission of <i>T. gondii</i> strains isolated from French Guiana.

a<p>Cumulative mortality of CD1 mice infected intraperitoneally with 10, 100, and 1000 parasites/mouse (5 mice/dose, 15 mice/strain).</p>b<p>Oral transmission using CD1 mice infected orally with 20 tissue cysts/mice (5 mice/strain).</p>C<p>Challenge of cats with 100–800 tissue cysts/cat.</p>d<p>Titer, generally considered positive when ≥25.</p>e<p>Unique genotype other than type I, II, and III.</p>f<p>related to type I allele.</p>g<p>negative by bioassay in mice.</p><p>nd, not done.</p><p>Sacrificed at day 10 prior to development of positive titer.</p><p>Virulence and transmission of <i>T. gondii</i> strains isolated from French Guiana.</p

Distribution of strains from French Guiana.

<p>Map of French Guiana shows locations of strains collected from the wild Neotropical rainforest (7 strains, green points) vs. anthropized environment (12 strains, red points). Of the 7 wild strains, only 3 (TgH18003, TgH18007, and TgH18008) were mapped with precision in the rainforest. Because information is lacking for the remaining 4 wild strains in the Neotropical rainforest (TgH18001, TgH18002, TgH00002, and TgH00009), they are shown as a large green point in the center of the rainforest area. For strains from the anthropized environment, each point corresponds to the localization of one or more isolates sharing the same geographic origin.</p

Genotyping of French Guiana <i>T. gondii</i> strains using PCR-RFLP markers.

a<p>Allele types are based on patterns seen in clonal type I, II and III strains.</p>b<p>The marker <i>3′-SAG2</i> and <i>Alt.SAG2</i> target different DNA fragments in the <i>SAG2</i> gene.</p>c<p>Genotype based on combined genetic profile characterized by the digestion pattern using both enzymes.</p>d<p>designates an unique allele.</p>e<p>Anthropized (A) vs. wild (W) strains.</p><p>Genotyping of French Guiana <i>T. gondii</i> strains using PCR-RFLP markers.</p

Analysis of <i>T. gondii</i> strains based on microsatellite markers.

<p>Strains isolated from anthropized environment (red) vs. wild Neotropical rainforest of French Guiana were compared to type strains representing the major haplogroups (HG). Neighbor-joining analysis of 15 microsatellite markers, displayed as an unrooted tree drawn using MEGA. Anthropized strains grouped with HG3 and HG9, while wild strains were deep branching and associated with a variety of divergent HGs. Color-coding indicates the region of origin: anthropized environment (red), wild Neotropical rainforest (green), HG reference strains (black).</p

Additional file 1 of Intraoperative electron radiotherapy in early invasive ductal breast cancer: 6-year median follow-up results of a prospective monocentric registry

Additional file 1. Supplementary information on the surgical and radiotherapy techniques