Tyrosine/Collagen and Tryptophan/Collagen ratios on osteocyte and surrounding matrix for the A25 group.

<p><b>A</b>: Comparison between osteocyte and matrix for the Tyrosine/Collagen ratio. <b>B</b>: Comparison between osteocyte and matrix for the Tryptophan/Collagen ratio. shows a significant difference between the ratios (p<0.05). Ratios are expressed as mean ± standard deviation (sd).</p

UV emission peak intensity ratios in osteocyte and surrounding matrix ROIs for the three different groups.

<p><b>A</b>: Tyrosine/Collagen ratio in osteocyte ROI. <b>B</b>: Tyrosine/Collagen ratio in matrix ROI. <b>C</b>: Tryptophan/Collagen ratio in osteocyte ROI. <b>D</b>: Tryptophan/Collagen ratio in matrix ROI. <b>E</b>: Tyrosine/Collagen ratio in osteocyte ROI. <b>F</b>: Tyrosine/Collagen ratio in matrix ROI. shows a significant difference between the groups. Ratios are expressed as mean ± standard deviation (sd).</p

UV spectroscopy results from osteocyte and surrounding matrix.

<p><b>A</b>: Transmission microscopy image showing ROIs, white scale bar 10 µm. <b>B</b>: UV fluorescence spectrum originating from osteocyte ROI pixel “o”. <b>C</b>: UV fluorescence spectrum originating from matrix ROI pixel “m”. <b>D</b>: Deconvolution of spectrum “m” into three Gaussians corresponding to tyrosine (a1), tryptophan (a2), and collagen (a3).</p

Bone sample ROI investigated on transmission image obtained on Synchrotron UV beamline.

<p>Tibia sections of 300 µm were cut. Magnifications (×50, ×100, ×200) represent histologic section after toluidine blue staining (white scale bars 80, 40, 20 µm, respectively). Magnification ×400 represents visible confocal microscopy acquired on UV beamline (the white scale bar indicates 10 µm).</p

Comparison of the UV spectroscopy Tyrosine/Collagen (Tyr/Coll) and Tryptophan/Collagen (Trp/Coll) ratios in osteocyte and matrix ROI for the A25 (n = 8), A35 (n = 8) and Control groups (n = 11).

<p>NS: non significant difference between ratios. Data were expressed as mean ± standard deviation (sd).</p