8 research outputs found
A fluorescent probe for imaging symmetric and asymmetric cell division in neurosphere formation
We report here a novel fluorescent chemical probe CDy5 which stains distinct neural stem/progenitor cells (NSPCs) by binding to acid ceramidase in mouse neurospheres. CDy5 is distributed evenly or unevenly to the daughter cells during multiple mitoses enabling the live imaging of symmetric and asymmetric divisions of isolated NSPCs.113sciescopu
Investigating the Spatial Distribution of Integrin β<sub>1</sub> in Patterned Human Mesenchymal Stem Cells Using Super-Resolution Imaging
Lineage commitment of human mesenchymal
stem cells (hMSCs) could
be directed through micro/nanopatterning of the extracellular matrix
(ECM) between cells and substrate. Integrin receptors, integrator
of the ECM and cell cytoskeleton, function as molecular bridges linking
cells to different biophysical cues translated from patterned ECM.
Here we report the distinct recruitment of active integrin β<sub>1</sub> (ITG-β<sub>1</sub>) in hMSCs when they were committed
toward the cardiomyogenic lineage on a micropatterned surface. In
addition, a systematic study of the distribution of ITG-β<sub>1</sub> was performed on focal adhesions (FAs) using a direct stochastic
optical reconstruction microscopy (dSTORM) technique, a super-resolution
imaging technique to establish the relationship between types of integrin
expression and its distribution pattern that are associated with cardiomyogenic
differentiation of hMSCs. We ascertained that elongated FAs of ITG-β<sub>1</sub> expressed in patterned hMSCs were more prominent than FAs
expressed in unpatterned hMSCs. However, there was no significant
difference observed between the widths of FAs from both experimental
groups. It was found in patterned hMSCs that the direction of FA elongation
coincides with cell orientation. This phenomenon was however not observed
in unpatterned hMSCs. These results showed that the biophysical induction
methods like FAs patterning could selectively induce hMSCs lineage
commitment via integrin-material interaction