75 research outputs found
Subcellular metabolite and lipid analysis of \u3ci\u3eXenopus laevis\u3c/i\u3e eggs by LAESI mass spectrometry
Xenopus laevis eggs are used as a biological model system for studying fertilization and early embryonic development in vertebrates. Most methods used for their molecular analysis require elaborate sample preparation including separate protocols for the water soluble and lipid components. In this study, laser ablation electrospray ionization (LAESI), an ambient ionization technique, was used for direct mass spectrometric analysis of X. laevis eggs and early stage embryos up to five cleavage cycles. Single unfertilized and fertilized eggs, their animal and vegetal poles, and embryos through the 32-cell stage were analyzed. Fifty two small metabolite ions, including glutathione, GABA and amino acids, as well as numerous lipids including 14 fatty acids, 13 lysophosphatidylcholines, 36 phosphatidylcholines and 29 triacylglycerols were putatively identified. Additionally, some proteins, for example thymosin β4 (Xen), were also detected. On the subcellular level, the lipid profiles were found to differ between the animal and vegetal poles of the eggs. Radial profiling revealed profound compositional differences between the jelly coat vitelline/plasma membrane and egg cytoplasm. Changes in the metabolic profile of the egg following fertilization, e.g., the decline of polyamine content with the development of the embryo were observed using LAESI-MS. This approach enables the exploration of metabolic and lipid changes during the early stages of embryogenesis
Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest
Bacteria belonging to the family Brevibacterieae are ubiquitous Gram positive organisms that are responsible for the feet odour and cheese aroma. Brevibacterium mcbrellneri is a relatively new member belonging to Brevibac- terieae. In the current manuscript we discuss isolation of biologically active metabolites from Brevibacterium mcbrellneri. Two aromatic esters were isolated from Brevibacterium mcbrellneri by “Bioassay guided fractiona- tion strategy” and identified as di-(2-ethylhexyl) phthalate and dibutyl phthalate by chemical characterization using biophysical techniques. The phthalate compounds show broad spectrum antibacterial activity and mosquito larvi-cidal activity. Mosquito larvicidal activity has been attributed to inhibition of
acetylcholinesterase enzyme activity. These compounds were found to be cytotoxic in multiple cell lines causing cell cycle arrest in G1 phase
Direct Detection of Diverse Metabolic Changes in Virally Transformed and Tax-Expressing Cells by Mass Spectrometry
International audiencen.
Chiral discrimination of α-amino acids by the DNA triplet GCA
The DNA triplet GCA is successfully used for the first time as a chiral selector for the chiral discrimination and optical purity measurement of some α-amino acids by investigating the collision-induced dissociation spectra of the sodiated ternary complex ion formed by electrospray ionization
A novel copper (II)/tin (II) reagent for aqueous carbonyl allylation: in situ diagnostics of reactive organometallics in water
A convenient and synthetically attractive protocol for the allylation of aldehydes using stannous chloride and catalytic cupric chloride in dichloromethane/water is described. In situ probing provides indirect (NMR, CV) and direct (MS) evidence for the copper(I)-catalyzed formation of an allyltrihalostannane intermediate in very high concentration in water
A Novel Copper(II)/Tin(II) Reagent for Regio- and Chemoselective Carbonyl Propargylation
Differential interactions of isomeric amino sugars with insulin studied under electrospray ionisation mass spectrometry
Protein-ligand interactions were studied for bovine insulin-amino sugar systems under electrospray ionisation mass spectrometry conditions. The isomeric amino sugars showed differences in the relative abundance of 1:1 protein-ligand complex formation. The electrospray ionisation and tandem mass spectrometry results of the complex clearly demonstrated that the differences in the interaction of isomeric sugars with insulin are mainly due to the differences in their gas-phase basicity. The same phenomenon is replicated in the formation of complexes between insulin and other ligands, such as amino acids, as well as in the binding of the amino sugars with amyloid β 1-40 peptide
Chiral discrimination of drugs by DNA tetranucleotides under electrospray ionisation conditions
The DNA tetranucleotides, extended versions of GCA at the 3'-end or 5'-end, were used as chiral selectors for the chiral discrimination of atenolol, DOPA, tamsulosin, valacyclovir and zolmitriptan. Chiral discrimination was achieved by investigating the collision-induced dissociation spectra of the [X+Y-2H]2- ion generated by electrospraying a solution mixture of tetranucleotide (X) and R- or S-analyte drug (Y). The relative abundances of the precursor ion and the product ion, resulting from the loss of drug, were considered for measuring the degree of chiral discrimination. Among all the tetranucleotides studied, AGCA showed the highest chiral discrimination. The present study emphasised the position of an adenine base in the tetranucleotide in chiral discrimination. The suitability of the method for the measurement of optical purity was also demonstrated in the case of zolmitriptan
Generation and Characterization of Distonic Dehydrophenoxide Radical Anions under Electrospray and Atmospheric Pressure Chemical Ionizations
We have explored the possibilities of generating radical anions under electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) conditions. By using different sets of ortho-, meta-, and para-isomers of nitrobenzoic acids, methylphenols, and nitrophenols, and m-, and p-isomers of hydroxybenzaldehydes and hydroxyacetophenones as the precursor molecules, we have successfully generated the isomeric distonic dehydrophenoxide radical anions (m/z 92) using the ESI process by applying relatively high capillary voltages, the in-source dissociation (ISD) condition. Under the same conditions, the o-hydroxybenzaldehyde and the o-hydroxyacetophenone yielded the even-electron dehydrophenoxide anion (m/z 93) due to the well-known ortho-effect. The distonic phenoxide radical anions at m/z 92 were also generated under APCI-ISD conditions by using m- and p-isomers of nitrobenzaldehydes and nitroacetophenones. While the o-nitrobenzaldehyde and the o-nitroacetophenone mainly yielded the phenoxide anion at m/z 93, due to the ortho-effect. The collision-induced dissociation (CID) experiments of all the anionic precursor molecules formed from either ESI or APCI produced comparable mass spectra as those observed in the ESI-ISD or the APCI-ISD experiments. The radical anions at m/z 92 reacted with CO2 and O2 to form the CO2 adduct and the oxygen atom abstraction product, respectively, revealing the dual-character of the distonic radical anions, the phenide ion and the phenyl radical. Computational studies support the results of the ion-molecule reactions
Chiral discrimination of α-amino acids by the DNA triplet GCA using amino acids as a co-selector
The DNA triplet GCA is successfully used as a chiral selector for the chiral discrimination of amino acids using amino acids themselves as a co-selector. Chiral discrimination was achieved by investigating the collision-induced dissociation spectra of the [XA + XR + 2Y - 2H]2- ion generated by electrospraying a mixture of analyte amino acid (XA), reference amino acid (XR) and GCA (Y). The relative abundances of fragment ions resulting from the competitive loss of reference and XA's are considered for measuring the degree of chiral discrimination. GCA successfully shows D-selectivity for all the amino acids, except Tyr and Lys. The success of the method lies in the selection of a suitable 10R that has closer GCA binding affinity to that of analyte. The degree of discrimination by GCA is improved in the presence of the reference, and the chirality of the reference does not change the selectivity of GCA. The suitability of the method for the measurement of optical purity is also demonstrated
- …