1 research outputs found
Colorimetric and Fluorometric Assays Based on Conjugated Polydiacetylene Supramolecules for Screening Acetylcholinesterase and Its Inhibitors
Polydiacetylene
supramolecules (PDAs) are unique sensing materials. Upon environmental
stimulation, blue PDAs can undergo a colorimetric transition from
blue to red accompanied by fluorescence enhancement. In this paper,
we report a new PDA system polymerized from a mixed liposome comprising
2-(2-(2-hydroxyethoxy)ethoxy)ethyl pentacosa-10,12-diynoate and pentacosa-10,12-diynoic
acid at a 3:7 ratio. The PDA system provided new colorimetric and
fluorometric assay methods for screening acetylcholinesterase and
its inhibitors through three processes. First, myristoylcholine reacted
with PDAs, which then underwent colorimetric and fluorometric transition.
Second, acetylcholinesterase catalyzed the hydrolysis of myristoylcholine
into tetradecanoic acid, which reduced the myristoylcholine concentration
and led to faded color and fluorescence. Third and last, acetylcholinesterase
inhibitors retarded the activity of acetylcholinesterase, thereby
inducing the recovery of color and fluorescence