SIMULATION OF VEHICULAR FRONTAL CRASH-TEST

This paper “SIMULATION OF FRONTAL CRASH-TEST” The simulation of vehicle crashes by using computer softwares has become an indispensible tool for shortening automobile development time and lowering costs. It also has huge impact on the crashworthiness of an automobile. This work reports on the simulated crash test of an automobile. The objective of this work is to simulate a frontal impact crash of an automobile and validate the results. The aim is also to alter some of the materials of the components with a view to reduce the forces experienced during the crash. Computer models were used to test the crash characteristics of the vehicle in the crash. The model used here was that of a Chevrolet C1500 pick-up truck. The software used for the simulation is LS-DYNA. It is widely used by the automotive industry to analyze vehicle designs. It accurately predicts a car\u27s behavior in a collision. The results obtained by the simulation were then validated by comparing it with the test results of the same test performed by the NCAC (National Crash Analysis Center)

Depression, anxiety, and stress among undergraduate dental students in Hyderabad City, Telangana, India: A cross-sectional study

Introduction: Increased levels of psychological disturbances such as depression, anxiety, and stress (DAS) among dental students affect the way these students take care of patients. Aim: The aim of this study is to assess DAS among undergraduate dental students in Hyderabad city, Telangana, India. Materials and Methods: A short version of depression, anxiety, and stress scale was distributed to undergraduate dental students in four dental colleges. Comparison among the variables was done using ANOVA and Independent t-test. Results: The study group comprised 200 (23.7%) males and 645 (76.3%) females. The overall mean DAS score and its dimensions were not significant based on gender. Married students showed significantly more DAS compared to unmarried (P < 0.05). When the year of study was considered for all colleges together, the overall mean DAS score and its individual dimensions score were significantly high among III year students followed by IV, I, and II years (P < 0.05). Conclusion: Clinical years were more stressful than the nonclinical years. This suggests a need for special attention to the structure of the clinical program, particularly at the point of transition from the preclinical to the clinical phase

DNA detection in tooth exposed to different temperatures: An in vitro study

Introduction: Human identification is an important field of study and research in forensic science and aims at establishing human identity. Several biological materials such as bone, hair, a biopsy sample, saliva and blood have been employed in isolation of DNA for human identification. It is possible to obtain DNA from virtually all human body tissues with variations in the quantity and quality of the DNA extracted from each tissue. Aims and Objectives: A study was carried out in our department to detect the presence of DNA from burnt teeth samples at various temperatures and to highlight the importance of DNA obtained from tooth in identifying a deceased in fire accidents. Materials and Methods: The work included 13 extracted teeth of patients who were indicated for therapeutic extraction and those who were diagnosed clinically and radiographically with caries and periodontitis who were indicated for extraction. Out of the 13 extracted teeth, two were decayed (One had class I dental caries C 1 and the other was grossly decayed C 2 ), four were periodontally compromised teeth and the other seven were therapeutically extracted. The freshly extracted teeth were immediately subjected to varying temperatures, from 100°C to 800°C using a Delta burnout furnace for 15-20 minutes. They were cryogenically crushed using a mortar and pestle to make samples of the tooth, which were analysed for DNA. Results: When teeth were incinerated from 100°C-800°C, genomic DNA was obtained only between 100°C and 300°C whereas it was not obtained above this temperature. When the teeth were incinerated from 300°C to 800°C mtDNA was extracted from 300°C to 700°C, but no DNA was obtained above 700°C. Conclusion: Teeth are good sources for DNA, even in cases where the specimens are highly decomposed

High affinity and covalent-binding microtubule stabilizing agents show activity in chemotherapy-resistant acute myeloid leukemia cells

16 p.-5 fig.-1 tab.-1 fig. supl.Treatment failure in acute myeloid leukemia (AML) is frequently due to the persistence of a cell population resistant to chemotherapy through different mechanisms, in which drug efflux via ATP-binding cassette (ABC) proteins, specifically P-glycoprotein, is one of the most recognized. However, disappointing results from clinical trials employing inhibitors for these transporters have demonstrated the need to adopt different strategies. We hypothesized that microtubule targeting compounds presenting high affinity or covalent binding could overcome the effect of ABC transporters. We therefore evaluated the activity of the high-affinity paclitaxel analog CTX-40 as well as the covalent binder zampanolide (ZMP) in AML cells. Both molecules were active in chemosensitive as well as in chemoresistant cell lines overexpressing P-glycoprotein. Moreover, ZMP or CTX-40 in combination with daunorubicin showed synergistic killing without increased in vitro hematopoietic toxicity. In a primary AML sample, we further demonstrated that ZMP and CTX-40 are active in progenitor and differentiated leukemia cell populations. In sum, our data indicate that high affinity and covalent-binding anti-microtubule agents are active in AML cells otherwise chemotherapy resistant.This work was supported by the Raymond and Beverly Sackler Scholar (L.C.), the Irma T. Hirschl Trust Award (L.C.) and the NSFC (Grant No. 30930108 to W.F.).Peer reviewe