COMPARISON OF ELISA AND RT-PCR FOR THE DETECTION OF PEANUT BUD NECROSIS VIRUS IN ONION (ALLIUM CEPA.L)

ABSTRACT: Peanut bud necrosis virus (PBNV) is an important re-emerging viral pathogen in onion (Allium cepa L.) in India. The virus transmitted by thrips vectors; it belongs to the genus Tospovirus and family Bunyaviridae. The onion crop infected by PBNV and it is a major problem in Southern India. This paper presents the comparison of DAC-ELISA and RT-PCR in the detection of PBNV infected onion samples from South India. The PBNV suspected onion samples (n=145) were collected in the major growing areas of Andhra Pradesh, Tamil Nadu and Karnataka states from South India. Among these collected onion samples, Seventy five samples (51.72%) were confirmed as PBNV infected by DAC-ELISA using the PBNV specific antiserum and in RT-PCR method one hundred twenty four samples (85.51%) were amplified (~800bp) by using the PBNV-CP gene specific primers. In comparison studies the RT-PCR method has added the advantage that it is more sensitive than the DAC-ELISA in the detection of PBNV in onion

Some Special Classes of Semirings

In this paper, we discuss the structure of some special classes of semirings. Here the properties of Viterbi semirings; PRD semirings satisfying the identity ab + a = a, for all a, b in S and semirings satisfying the identity a + ab + a = a, for all a, b in S are studied. We proved that (S, +, •) is a semiring satisfying the identity a + ab + a = a, in which if S contains the multiplicative identity which is also an additive identity, then S is a multiplicatively subidempotent semiring

Group structure in circle theorem

A general method to discuss the potential flow past two intersecting circles is presented. This is done by introducing two operators L and M, which generate a group G. A procedure called closure is introduced, which determines the order of the group and the angles of intersection of the two circles