24 research outputs found
Catalytic combustion of methane on Co/MgO: characterisation of active cobalt sites
A series of Co/MgO catalysts with 3–12 wt.% Co were prepared by impregnation and calcined at 1073 K for 10 h. The catalytic behaviour of these samples toward CH4 combustion was found to increase with cobalt loading, though a plateau was reached at ca. 9 wt.% Co content. Bulk characterisation was carried out using XRD, TPR and Raman spectroscopy, and showed that the solids were made up of a CoO–MgO solid solution and a MgO phase. A detailed examination of their surfaces was achieved through FTIR spectroscopy of adsorbed CO probe molecules, which indicated that at low cobalt loadings only a small proportion of the Co going into the solid solution was present on exposed faces as either Co2+ oxo-species or pentacoordinated Co2+. However, as the cobalt content of the samples increased, a larger amount was exposed on the surface. This effect levelled off at 9 wt.% Co, after which the increase in exposed Co2+ sites was countered by the masking effect of islands of MgO. In addition, at high cobalt loadings (9 and 12 wt.%) Co formed small clusters which showed bulk CoO-like behaviour. Consequently, the benefit of having surface Co2+ species was balanced by the clustering effect of these species and the presence of MgO islands, negating their contribution to the overall catalytic activity of the samples.Fil: Ulla, Maria Alicia del H.. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y PetroquĂmica ; ArgentinaFil: Spretz, R.. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y PetroquĂmica ; ArgentinaFil: Lombardo, Eduardo Agustin. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y PetroquĂmica ; ArgentinaFil: Daniell, W.. Ludwig Maximilians Universitat; AlemaniaFil: Knözinger, H.. Ludwig Maximilians Universitat; Alemani
A new approach for the treatment of CLL using chlorambucil/hydroxychloroquine-loaded anti-CD20 nanoparticles
Current approaches for the treatment of chronic lymphocytic leukemia (CLL) have greatly improved the prognosis for survival, but some patients remain refractive to these therapeutic regimens. Hence, in addition to reducing the long-term sideeffects of therapeutics for all leukemia patients, there is an urgent need for novel therapeutic strategies for difficult-to-treat leukemia cases. Due to the cytotoxicity of drugs, the major challenge currently is to deliver the therapeutic agents to neoplastic cells while preserving the viability of non-malignant cells. In this study, we propose a therapeutic approach in which high doses of hydroxychloroquine and chlorambucil were loaded into biodegradable polymeric nanoparticles coated with an anti-CD20 antibody.We first demonstrated the ability of the nanoparticles to target and internalize in tumor B-cells. Moreover, these nanoparticles could kill not only p53-mutated/deleted leukemia cells expressing a low amount of CD20, but also circulating primary cells isolated from chronic lymphocytic leukemia patients. The safety of these nanoparticles was also demonstrated in healthy mice, and their therapeutic effects were shown in a new model of aggressive leukemia. These results showed that anti-CD20 nanoparticles containing hydroxychloroquine and chlorambucil can be effective in controlling aggressive leukemia and provided a rationale for adopting this approach for the treatment of other B-cell disorders. [Figure not available: see fulltext.
Convection enhanced delivery and \u3ci\u3ein vivo\u3c/i\u3e imaging of polymeric nanoparticles for the treatment of malignant glioma
A major obstacle to the management of malignant glioma is the inability to effectively deliver therapeutic agent to the tumor. In this study, we describe a polymeric nanoparticle vector that not only delivers viable therapeutic, but can also be tracked in vivo using MRI. Nanoparticles, produced by a non-emulsion technique, were fabricated to carry iron oxide within the shell and the chemotherapeutic agent, temozolomide (TMZ), as the payload. Nanoparticle properties were characterized and subsequently their endocytosis-mediated uptake by glioma cells demonstrated. Convection enhanced delivery (CED) can disperse nanoparticles through the rodent brain and their distribution is accurately visualized by MRI. Infusion of nanoparticles does not result in observable animal toxicity relative to control. CED of TMZ bearing nanoparticles prolongs the survival of animals with intracranial xenografts compared to control. In conclusion, the described nanoparticle vector represents a unique multifunctional platform that can be used for image-guided treatment of malignant glioma
Decoy receptor DcR1 is induced in a p50/Bcl3-dependent manner and attenuates the efficacy of temozolomide
Temozolomide is used widely to treat malignant glioma but the overall response to this agent is generally poor. Resistance to DNA damaging drugs such as temozolomide has been related to the induction of anti-apoptotic proteins. Specifically, the transcription factor NF-ÎşB has been suggested to participate in promoting the survival of cells exposed to chemotherapy. To identify factors that modulate cytotoxicity in the setting of DNA damage, we used an unbiased strategy to examine the NF-ÎşB-dependent expression profile induced by temozolomide. By this route, we defined the decoy receptor DcR1 as a temozolomide response gene induced by a mechanism relying upon p50/NF-ÎşB1. A conserved NF-ÎşB binding sequence (ÎşB-site) was identified in the proximal promoter and demonstrated to be required for DcR1 induction by temozolomide. Loss-of-function and gain-of-function studies reveal that the atypical IÎşB protein, Bcl3, is also required for induction of DcR1 by temozolomide. Mechanistically, DcR1 attenuates temozolomide efficacy by blunting activation of the Fas receptor pathway in p53+/+ glioma cells. Intracranial xenograft studies show that DcR1 depletion in glioma cells enhances the efficacy of temozolomide. Taken together, our results show how DcR1 upregulation mediates temozolomide resistance, and provide a rationale for DcR1 targeting as a strategy to sensitize gliomas to this widely used chemotherapy
Decoy receptor DcR1 is induced in a p50/Bcl3-dependent manner and attenuates the efficacy of temozolomide
Temozolomide is used widely to treat malignant glioma but the overall response to this agent is generally poor. Resistance to DNA damaging drugs such as temozolomide has been related to the induction of anti-apoptotic proteins. Specifically, the transcription factor NF-ÎşB has been suggested to participate in promoting the survival of cells exposed to chemotherapy. To identify factors that modulate cytotoxicity in the setting of DNA damage, we used an unbiased strategy to examine the NF-ÎşB-dependent expression profile induced by temozolomide. By this route, we defined the decoy receptor DcR1 as a temozolomide response gene induced by a mechanism relying upon p50/NF-ÎşB1. A conserved NF-ÎşB binding sequence (ÎşB-site) was identified in the proximal promoter and demonstrated to be required for DcR1 induction by temozolomide. Loss-of-function and gain-of-function studies reveal that the atypical IÎşB protein, Bcl3, is also required for induction of DcR1 by temozolomide. Mechanistically, DcR1 attenuates temozolomide efficacy by blunting activation of the Fas receptor pathway in p53+/+ glioma cells. Intracranial xenograft studies show that DcR1 depletion in glioma cells enhances the efficacy of temozolomide. Taken together, our results show how DcR1 upregulation mediates temozolomide resistance, and provide a rationale for DcR1 targeting as a strategy to sensitize gliomas to this widely used chemotherapy
Operation of FCC with mixtures of regenerated and deactivated catalyst
The operation of FCC with mixtures of coked and regenerated catalyst was studied with a riser simulator reactor on two equilibrium catalysts at 550â—¦C. The coked catalysts maintain an activity level that enables them to be used in the mixtures. The catalytic performances of the regenerated catalysts were used as references against which the behaviors of 25:75 and 50:50 (coked:regenerated) mixtures were compared. It was observed that overall catoil has to be increased to maintain conversion. While the yields of gases, gasoline and LCO showed to be independent of the operative mode, changes were observed in the selectivity to light olefinsC4?C6that are mainly due to changes in the yields of the isoparaffins in the groups. In turn, these changes could be the consequence of the resulting density of paired acid sites in the zeolite components on hydrogen transfer reactions, due to the contributions by the coked and regenerated portions of catalysts. Coke yields in mixtures of coked and regenerated catalysts are not higher, which would allow increasing catalyst circulation without impacting on heat balance. The particularities of this new operation of FCC are very dependent on catalyst properties.Fil: Spretz, R.. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y PetroquĂmica ; ArgentinaFil: Sedran, Ulises Anselmo. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y PetroquĂmica ; Argentin
Fe/MgO formulations for the catalytic combustion of methane
Fe/MgO catalysts containing between 1.5 and 9 wt% iron were prepared by impregnation and calcined at 1073 K for 10 h. The solids were characterized by XRD and Raman and Mössbauer spectroscopies. They all showed that the solids are made up of MgFe2O4 spinel clusters and Fe3+ dispersed in the MgO matrix. The concentrations of MgFe2O4 and Fe3+ were calculated from the Mössbauer data. The former increases by a factor of 23 while the latter remains almost constant when the iron loading varies between 1.5 and 9 wt%. The catalytic activity correlates with the spinel weight fraction in the Fe/MgO system. Both the SEM micrographs and the EPMA analyses of the catalysts indicate that the solid surface is made up of exposed patches of MgFe2O4 and MgO. This surface feature explains the plateau observed when the reaction rate constant is plotted versus the iron loading.Fil: Spretz, R.. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y PetroquĂmica ; ArgentinaFil: Marchetti, Sergio Gustavo. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Centro de InvestigaciĂłn y Desarrollo en Ciencias Aplicadas ; ArgentinaFil: Ulla, Maria Alicia del H.. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y PetroquĂmica ; ArgentinaFil: Lombardo, Eduardo Agustin. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y PetroquĂmica ; Argentin
The nature of the active species on the Fe/MgO in the combustion of methane
A series of Fe/MgO solids (1.5–9.0 wt% Fe) calcined at 1073 K is shown to be made up of MgFe2O4 and Fe3+ dispersed in the MgO matrix. Mössbauer spectroscopy provided the data to quantify both species. The rate data for methane combustion correlates with the spinel content but not with the Fe3+ concentration. The locus of activity is discussed in terms of both the solid features and existing literature.Fil: Spretz, R.. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y PetroquĂmica ; ArgentinaFil: Marchetti, Sergio Gustavo. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Centro de InvestigaciĂłn y Desarrollo en Ciencias Aplicadas ; ArgentinaFil: Ulla, Maria Alicia del H.. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y PetroquĂmica ; ArgentinaFil: Lombardo, Eduardo Agustin. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y PetroquĂmica ; Argentin
Convection enhanced delivery and \u3ci\u3ein vivo\u3c/i\u3e imaging of polymeric nanoparticles for the treatment of malignant glioma
A major obstacle to the management of malignant glioma is the inability to effectively deliver therapeutic agent to the tumor. In this study, we describe a polymeric nanoparticle vector that not only delivers viable therapeutic, but can also be tracked in vivo using MRI. Nanoparticles, produced by a non-emulsion technique, were fabricated to carry iron oxide within the shell and the chemotherapeutic agent, temozolomide (TMZ), as the payload. Nanoparticle properties were characterized and subsequently their endocytosis-mediated uptake by glioma cells demonstrated. Convection enhanced delivery (CED) can disperse nanoparticles through the rodent brain and their distribution is accurately visualized by MRI. Infusion of nanoparticles does not result in observable animal toxicity relative to control. CED of TMZ bearing nanoparticles prolongs the survival of animals with intracranial xenografts compared to control. In conclusion, the described nanoparticle vector represents a unique multifunctional platform that can be used for image-guided treatment of malignant glioma