571 research outputs found

    Ich und die Kamera : Film ohne Dramaturgie am Beispiel des Tagebuchfilms "November 1-30" von Jan Peters

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    Ein Film ohne Dramaturgie? Das kann nur ein Dokumentarfilm sein. Aber wo fangen die Kriterien für einen Dokumentarfilm an und wo enden die Möglichkeiten der Dramaturgie? Die Kategorisierung möchte ich mir ausnahmsweise einmal einfach machen und den Dokumentarfilm als ein filmisches Genre bezeichnen, das sich vom fiktionalen Erzählen vor allem dadurch abgrenzt, dass es sein Material unmittelbar dem Leben entnimmt – ohne den Umweg des Drehbuchs und der Nachinszenierung. Das hat meistens zur Folge, dass man in einem Dokumentarfilm genau das nicht hat, was einen Spielfilm so stark macht: die Schlüsselszenen – Szenen also, die die Organisation einer funktionalen Dramaturgie so einfach machen

    Equations of Motion in a Rotating Noninertial Reference Frame

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    Measurement is an essential part of empirical research. As such, understanding whether the frame of reference in which a measurement occurs is inertial is essential for accurate data. As a rotating sphere, Earth is a non-inertial frame of reference and gives rise to fictitious forces. These forces are derived through vector algebra and further solved through matrix differential equations. The final solution for how velocity evolves over time results in sinusoidal functions with a period of 24 hours for Earth. To test the equations further, rational scenarios are proposed for different locations on the surface of Earth involving different initial velocities, resulting in expected behavior. It is also important to demonstrate that the complete set of solutions collapse into basic kinematic equations for an inertial frame of reference under gravity when rotation of the system is absent. Course: Math 488 – Senior Capstonehttps://commons.und.edu/es-showcase/1016/thumbnail.jp

    The Falling Sky: Words of a Yanomami Shaman - Davi Kopenawa and Bruce Albert

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    The interaction of source and post-event misinformation on the accuracy of eyewitness testimony

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    The recall of an event such as a robbery has been shown to be affected by how closely post-event information corresponds to what the witness actually saw. The purpose of this study was to investigate how the effect of misleading information may be influenced by expertise of the source of the post-event information. Results revealed that subjects recalled less accurately when they received misleading information in the narrative than when the information corresponded with what they had viewed, whereas the accuracy of the recall was unaffected by whether the witness in the narrative was an expert or a non-expert. Subjects rated the narrative witnesses as having equal credibility. This study suggests that the memory for events related to a crime-scene may be impaired by misleading post-event information, but is unaffected by the source of that misleading information when the sources differ in occupational expertise

    Advantages and Limitations of Direct PCR Amplification of Bacterial 16S-rDNA from Resected Heart Tissue or Swabs Followed by Direct Sequencing for Diagnosing Infective Endocarditis: A Retrospective Analysis in the Routine Clinical Setting

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    Infective endocarditis (IE) is a life-threatening disease that is associated with high morbidity and mortality. Its long-term prognosis strongly depends on a timely and optimized antibiotic treatment. Therefore, identification of the causative pathogen is crucial and currently based on blood cultures followed by characterization and susceptibility testing of the isolate. However, antibiotic treatment starting prior to blood sampling or IE caused by fastidious or intracellular microorganisms may cause negative culture results. Here we investigate the additional diagnostic value of broad-range PCR in combination with direct sequencing on resected heart tissue or swabs in patients with tissue or swab culture-negative IE in a routine clinical setting. Sensitivity, specificity, and positive and negative predictive values of broad-range PCR from diagnostic material in our patients were 33.3%, 76.9%, 90.9%, and 14.3%, respectively. We identified a total of 20 patients (21.5%) with tissue or culture-negative IE who profited by the additional application of broad-range PCR. We conclude that broad-range PCR on resected heart tissue or swabs is an important complementary diagnostic approach. It should be seen as an indispensable new tool for both the therapeutic and diagnostic management of culture-negative IE and we thus propose its possible inclusion in Duke’s diagnostic classification scheme

    Анализ эффективности методов борьбы с отложениями солей на примере месторождений Западной Сибири

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    В ходе работы изучены теоретические основы существующих методов борьбы с отложениями солей, при эксплуатации скважин на территории Западной Сибири проведен технико-экономический анализ внедрения метода борьбы с отложениями солей при эксплуатации скважин на типовых месторождениях.In the course of the work, the theoretical foundations of existing methods of control of salt deposits in the operation of wells in Western Siberia were studied, the technical and economic analysis of the implementation of the method of control of salt deposits in the operation of wells in typical fields was carried out

    Functional characterization of SlscADH1, a fruit-ripening associated short-chain alcohol dehydrogenase of tomato

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    A tomato short-chain dehydrogenase-reductase (SlscADH1) is preferentially expressed in fruit with a maximum expression at the breaker stage while expression in roots, stems, leaves and flowers is very weak. It represents a potential candidate for the formation of aroma volatiles by interconverting alcohols and aldehydes. The SlscADH1 recombinant protein produced in Escherichia coli exhibited dehydrogenase-reductase activity towards several volatile compounds present in tomato flavour with a strong preference for the NAD/NADH co-factors. The strongest activity was observed for the reduction of hexanal (Km = 0.175 mM) and phenylacetaldehyde (Km = 0.375 mM) in the presence of NADH. The oxidation process of hexanol and 1-phenylethanol was much less efficient (Kms of 2.9 and 23.0 mM, respectively), indicating that the enzyme preferentially acts as a reductase. However activity was observed only for hexanal, phenylacetaldehyde, (E)-2-hexenal and acetaldehyde and the corresponding alcohols. No activity could be detected for other aroma volatiles important for tomato flavour, such as methyl-butanol/methyl-butanal, 5-methyl-6-hepten-2-one/5-methyl-6-hepten-2-ol, citronellal/citronellol, neral/nerol, geraniol. In order to assess the function of the SlscADH1 gene, transgenic plants have been generated using the technique of RNA interference (RNAi). Constitutive down-regulation using the 35S promoter resulted in the generation of dwarf plants, indicating that the SlscADH1 gene, although weakly expressed in vegetative tissues, had a function in regulating plant development. Fruitspecific down-regulation using the 2A11 promoter had no morphogenetic effect and did not alter the aldehyde/alcohol balance of the volatiles compounds produced by the fruit. Nevertheless, SlscADH1-inhibited fruit unexpectedly accumulated higher concentrations of C5 and C6 volatile compounds of the lipoxygenase pathway, possibly as an indirect effect of the suppression of SlscADH1 on the catabolism of phospholipids and/or integrity of membranes
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