Phytochemical Studies of Momordica Charantia Fruit: Seed Extraction via Aqueous Enzymatic Extraction and Pulp Maceration

The edible fruits of Momordica charantia or bitter melons have been used for over a decade in folk medicine for treatment of diabetes and malaria. The seeds usually have been discarded without any further use, however, it’s can be a good source of biomass. Hence, this research aimed to isolate active constituents from Momordica charantia seeds after aqueous enzymatic extraction and pulp after maceration

Developement of cycloisomerization reactions for the synthesis of nitrogen or oxygen containing heterocycles

La catalyse, les solvants alternatifs et l'économie d'atome font partie des clés pour le développement de nouvelles stratégies durables de synthèse. Des hétérocycles comme les quinoléines substituées, par réaction de cycloisomérisation catalysée par le cuivre en utilisant les liquides ioniques, et les isoquinoléines carbonylées par réaction de carbonylation oxydante catalysée par le palladium dans le méthanol ont été synthétisées. La formation sélective de 1,3-dihydroisobenzofuranes et/ou 1H-isochromènes a été tentée par réaction de cycloisomérisation catalysée par différent métaux de transition. La synthèse d'hétérocycles à cinq chaînons, par exemple furanes, pyrroles et pyrrolin-4-ones à été mise au point par nouvelle réaction de cycloisomérisation catalysée par de sels de platine ou d'or en utilisant des diols, des amino alcools ou des α-amino-ynones dans le PEG sous activation micro-ondes. Les produits sont récupérés pur après une simple étape de précipitation-filtration. Les α-amino-ynones chiral sont synthétisée à partir de N-protégé carboxyanhydrides des aminoacides (UNCAs). Une réaction de iodocyclisation a été aussi développée pour obtenir de nouvelles structures hétérocycliques.Catalysis, alternative solvents and atom economy represent key areas for the sustainable development of versatile strategies in organic chemistry. Fused heterocycles, such as substituted quinolines by a cycloisomerization reaction using a recyclable catalytic system copper/ionic liquids and isoquinoline-4-carboxylic esters based on PdI2-catalyzed oxidative carbonylation were prepared. A selective 5-exo-dig or 6-endo-dig cyclization route to obtain 1,3-dihydroisobenzofurans and/or 1H-isochromenes was tested by metal transition cycloisomerization reaction of alkynylbenzyl alcohols in PEG. Five membered ring heterocycles such as furans, pyrroles and pyrrolin-4-ones were successfully obtained by a novel platinum or gold-catalyzed cycloisomerization reaction of diols, aminoalcohols or α-amino-ynones in poly(ethylene glycol) (PEG) under microwave irradiation. The heterocyclic systems were recovered pure after a simple precipitation-filtration work-up. The catalytic systems were studied by using the TEM and XPS techniques. The chiral α-amino-ynone substrates were prepared by an original method starting from N-protected carboxyanhydrides of amino acids (UNCAs). Also unprecedented results are reported in the area of iodocyclization to obtain chiral iodopyrrolin-ones

The role of endophytic bacteria in biosynthesis of important therapeutic Amaryllidaceae alkaloids

International audienceoccurring in such plants as Leucojum aestivum, are currentlyof great medical importance. Endophytes are microorganismsliving in plants without causing any damage for the plant. Recently,a trend in the biotechnology is the possibility to use the abilityof certain endophytic bacteria to produce medicinal substancesas well as their stimulating effect on the biosynthesis of secondarymetabolites under in vitro conditions. The objective of thisproject is to determine the possibilities of using endophyticbacteria colonizing Leucojum aestivum for the productionof Amaryllidaceae alkaloids

Developement of cycloisomerization reactions for the synthesis of nitrogen or oxygen containing heterocycles

La catalyse, les solvants alternatifs et l'économie d'atome font partie des clés pour le développement de nouvelles stratégies durables de synthèse. Des hétérocycles comme les quinoléines substituées, par réaction de cycloisomérisation catalysée par le cuivre en utilisant les liquides ioniques, et les isoquinoléines carbonylées par réaction de carbonylation oxydante catalysée par le palladium dans le méthanol ont été synthétisées. La formation sélective de 1,3-dihydroisobenzofuranes et/ou 1H-isochromènes a été tentée par réaction de cycloisomérisation catalysée par différent métaux de transition. La synthèse d'hétérocycles à cinq chaînons, par exemple furanes, pyrroles et pyrrolin-4-ones à été mise au point par nouvelle réaction de cycloisomérisation catalysée par de sels de platine ou d'or en utilisant des diols, des amino alcools ou des a-amino-ynones dans le PEG sous activation micro-ondes. Les produits sont récupérés pur après une simple étape de précipitation-filtration. Les a-amino-ynones chiral sont synthétisée à partir de N-protégé carboxyanhydrides des aminoacides (UNCAs). Une réaction de iodocyclisation a été aussi développée pour obtenir de nouvelles structures hétérocycliques.Catalysis, alternative solvents and atom economy represent key areas for the sustainable development of versatile strategies in organic chemistry. Fused heterocycles, such as substituted quinolines by a cycloisomerization reaction using a recyclable catalytic system copper/ionic liquids and isoquinoline-4-carboxylic esters based on PdI2-catalyzed oxidative carbonylation were prepared. A selective 5-exo-dig or 6-endo-dig cyclization route to obtain 1,3-dihydroisobenzofurans and/or 1H-isochromenes was tested by metal transition cycloisomerization reaction of alkynylbenzyl alcohols in PEG. Five membered ring heterocycles such as furans, pyrroles and pyrrolin-4-ones were successfully obtained by a novel platinum or gold-catalyzed cycloisomerization reaction of diols, aminoalcohols or a-amino-ynones in poly(ethylene glycol) (PEG) under microwave irradiation. The heterocyclic systems were recovered pure after a simple precipitation-filtration work-up. The catalytic systems were studied by using the TEM and XPS techniques. The chiral a-amino-ynone substrates were prepared by an original method starting from N-protected carboxyanhydrides of amino acids (UNCAs). Also unprecedented results are reported in the area of iodocyclization to obtain chiral iodopyrrolin-ones.MONTPELLIER-BU Sciences (341722106) / SudocSudocFranceF

Poly(ethylene glycol) as a reaction matrix in platinum- or gold-catalyzed cycloisomerization: a mechanistic investigation.

International audienceDesign for diversity: A new catalytic system based on PEG-3400 and a metal salt (Pt or Au) was designed to efficiently perform a cycloisomerization reaction under microwave irradiation, which gave diverse heterocycles in good to excellent yields, after a precipitation/filtration procedure (see scheme)

Diversity of natural products of the genera Curvularia and Bipolaris

International audienceCovering from 1963 to 2017.This review provides a summary of some secondary metabolites isolated from the genera Curvularia and Bipolaris from 1963 to 2017. The study has a broad objective. First to afford an overview of the structural diversity of these genera, classifying them depending on their chemical classes, highlighting individual examples of chemical structures. Also some information regarding their biological activities are presented. Several of the compounds reported here were isolated exclusively from endophytic and pathogenic strains in culture, while few from other sources such as sea Anemone and fish. Some secondary metabolites of the genus Curvularia and Bipolaris revealed a fascinating biological activities included: anti-malarial, anti-biofouling, anti-larval, anti-inflammatory, anti-oxidant, anti-bacterial, anti-fungal, anti-cancer, leishmanicidal and phytotoxicity. Herein, we presented a bibliography of the researches accomplished on the natural products of Curvularia and Bipolaris, which could help in the future prospecting of novel or new analogues of active metabolites from these two genera

Development of cycloisomerization reactions for the synthesis of nitrogen or oxygen containing heterocycles

Dottorato di ricerca in "Organic Materials of Pharmaceutical interest" XXIV Ciclo, a.a.2011Università della Calabria, Università Montpellie

Du végétal à l’homéopathie, intérêts des plantes carnivores en thérapeutique

International audienceLes plantes carnivores sont des végétaux insolites qui ont toujours intrigué les scientifiques.Leur principal emploi en thérapeutique concerne l’homéopathie, mais depuis plusieursannées, de nouvelles approches commencent à émerger

Montpellier - France

International audienceEndophytes are bacteria or fungi that invade living plant tissues without causing disease. As their host plant, endophytes also produce a wide variety of specialized metabolites. At first, we studied the endophytic fungal flora of medicinal plants from Sudan, its molecular characterization was carried out. Then we multiplied the fungus Curvularia papendorfi under controlled conditions. The phytochemical study of extracts of this fungus led to the isolation of 5 biomolecules including a new compound. A set of spectroscopic methods, 1D and 2D NMR, IR, UV and high-resolution mass spectrometry (HR-ESI-MS), were used to determine the structure of this new compound: 3,7,11,15 tetrahydroxy-18-hydroxymethyl-14,16,20,22,24-pentamethylhexacosa-4E, 8E, 12E, 16,18-pentaenoic acid. It showed moderate anti-bacterial activity against S. aureus with the IC50 value = 62.5 μg / mL and low cytotoxicity with the IC50 value > 100 μM against MCF7 cancer cells (human breast carcinoma)

Identification of biomolecules in Endophytes Fungi from Sudan’s medicinal plants

International audienceEndophytes are bacteria or fungi that invade living plant tissues without causing disease. As their host plant, endophytes also produce a wide variety of specialized metabolites. At first, we studied the endophytic fungal flora of medicinal plants from Sudan, its molecular characterization was carried out. Then we multiplied the fungus Curvularia papendorfi under controlled conditions. The phytochemical study of extracts of this fungus led to the isolation of 5 biomolecules including a new compound. A set of spectroscopic methods, 1D and 2D NMR, IR, UV and high-resolution mass spectrometry (HR-ESI-MS), were used to determine the structure of this new compound: 3,7,11,15 tetrahydroxy-18-hydroxymethyl-14,16,20,22,24-pentamethylhexacosa-4E, 8E, 12E, 16,18-pentaenoic acid. It showed moderate anti-bacterial activity against S. aureus with the IC50 value = 62.5 μg / mL and low cytotoxicity with the IC50 value > 100 μM against MCF7 cancer cells (human breast carcinoma)