718 research outputs found
Selectins mediate macrophage infiltration in obstructive nephropathy in newborn mice11See Editorial by Kipari and Hughes, p. 760.
Selectins mediate macrophage infiltration in obstructive nephropathy in newborn mice.BackgroundUrinary tract obstruction during development leads to tubular atrophy and causes interstitial fibrosis. Macrophage infiltration into the interstitium plays a central role in this process. Selectins, a family of three adhesion molecules, are involved in leukocyte recruitment to sites of inflammation and immune activity. We investigated the role of selectins in obstructive nephropathy in newborn mice.MethodsTriple selectin-deficient mice (EPL-/-), L-selectin deficient mice (L-/-) and wild type mice (WT) were subjected to complete unilateral ureteral obstruction (UUO) or sham operation within the first 48 hours of life, and were sacrificed 5 and 12 days later. Kidneys were removed, and sections were stained for macrophage infiltration (mAb F4/80), apoptosis (TUNEL), tubular atrophy (periodic acid-Schiff) and interstitial fibrosis (Masson trichrome).ResultsSelectin deficient mice showed a marked reduction in macrophage infiltration into the obstructed kidney compared to WT at day 5 and day 12 after UUO. Tubular apoptosis was strongly reduced in EPL-/- at day 5 after UUO, and in EPL-/- and L-/- at day 12 after UUO when compared to WT. The number of apoptotic tubular cells was correlated with macrophage infiltration, suggesting that macrophages stimulate tubular apoptosis in obstructive nephropathy. In addition, tubular atrophy and interstitial fibrosis were significantly diminished in EPL-/- and L-/- compared to WT at day 12 after UUO.ConclusionFollowing UUO, selectins mediate macrophage infiltration into the obstructed kidney, which in turn may induce tubular apoptosis, tubular atrophy and interstitial fibrosis
Identificação e resistência a antibióticos de gêneros bacterianos presentes em surubins criados em tanques-rede.
O surubim é uma espécie apreciada pelo mercado consumidor, entretanto, seu cultivo intensivo em tanques-rede pode trazer problemas de sanidade devido ao estresse dos peixes, caso não sejam utilizadas boas práticas de manejo. Neste trabalho, objetivou-se identificar e verificar a resistência aos antibióticos de gêneros bacterianos isolados de Pseudoplatystoma sp. criados em tanques-rede de baixo volume
Targeting Neutrophil Adhesive Events to Address Vaso-Occlusive Crisis in Sickle Cell Patients
Neutrophils are essential to protect the host against invading pathogens but can promote disease progression in sickle cell disease (SCD) by becoming adherent to inflamed microvascular networks in peripheral tissue throughout the body. During the inflammatory response, leukocytes extravasate from the bloodstream using selectin adhesion molecules and migrate to sites of tissue insult through activation of integrins that are essential for combating pathogens. However, during vaso-occlusion associated with SCD, neutrophils are activated during tethering and rolling on selectins upregulated on activated endothelium that line blood vessels. Recently, we reported that recognition of sLex on L-selectin by E-selectin during neutrophil rolling initiates shear force resistant catch-bonds that facilitate tethering to endothelium and activation of integrin bond clusters that anchor cells to the vessel wall. Evidence indicates that blocking this important signaling cascade prevents the congestion and ischemia in microvasculature that occurs from neutrophil capture of sickled red blood cells, which are normally deformable ellipses that flow easily through small blood vessels. Two recently completed clinical trials of therapies targeting selectins and their effect on neutrophil activation in small blood vessels reveal the importance of mechanoregulation that in health is an immune adaption facilitating rapid and proportional leukocyte adhesion, while sustaining tissue perfusion. We provide a timely perspective on the mechanism underlying vaso-occlusive crisis (VOC) with a focus on new drugs that target selectin mediated integrin adhesive bond formation
Searches for the Violation of Pauli Exclusion Principle at LNGS in VIP(-2) experiment
The VIP (Violation of Pauli exclusion principle) experiment and its follow-up
experiment VIP-2 at the Laboratori Nazionali del Gran Sasso (LNGS) search for
X-rays from Cu atomic states that are prohibited by the Pauli Exclusion
Principle (PEP). The candidate events, if they exist, will originate from the
transition of a orbit electron to the ground state which is already
occupied by two electrons. The present limit on the probability for PEP
violation for electron is 4.7 set by the VIP experiment. With
upgraded detectors for high precision X-ray spectroscopy, the VIP-2 experiment
will improve the sensitivity by two orders of magnitude.Comment: 5 pages, 3 figures, 1 table. Conference proceedings for oral
presentation at TAUP 2015, Torin
Beyond quantum mechanics? Hunting the 'impossible' atoms (Pauli Exclusion Principle violation and spontaneous collapse of the wave function at test)
The development of mathematically complete and consistent models solving the
so-called "measurement problem", strongly renewed the interest of the
scientific community for the foundations of quantum mechanics, among these the
Dynamical Reduction Models posses the unique characteristic to be
experimentally testable. In the first part of the paper an upper limit on the
reduction rate parameter of such models will be obtained, based on the analysis
of the X-ray spectrum emitted by an isolated slab of germanium and measured by
the IGEX experiment.
The second part of the paper is devoted to present the results of the VIP
(Violation of the Pauli exclusion principle) experiment and to describe its
recent upgrade. The VIP experiment established a limit on the probability that
the Pauli Exclusion Principle (PEP) is violated by electrons, using the very
clean method of searching for PEP forbidden atomic transitions in copper
Testing the Pauli Exclusion Principle for electrons at LNGS
High-precision experiments have been done to test the Pauli exclusion
principle (PEP) for electrons by searching for anomalous -series X-rays from
a Cu target supplied with electric current. With the highest sensitivity, the
VIP (VIolation of Pauli Exclusion Principle) experiment set an upper limit at
the level of for the probability that an external electron captured
by a Cu atom can make the transition from the 2 state to a 1 state
already occupied by two electrons. In a follow-up experiment at Gran Sasso, we
aim to increase the sensitivity by two orders of magnitude. We show proofs that
the proposed improvement factor is realistic based on the results from recent
performance tests of the detectors we did at Laboratori Nazionali di Frascati
(LNF).Comment: 8 pages, 5 figures, conference proceedings on TAUP 201
Spontaneously emitted X-rays: an experimental signature of the dynamical reduction models
We present the idea of searching for X-rays as a signature of the mechanism
inducing the spontaneous collapse of the wave function. Such a signal is
predicted by the continuous spontaneous localization theories, which are
solving the "measurement problem" by modifying the Schrodinger equation. We
will show some encouraging preliminary results and discuss future plans and
strategy.Comment: to be published in Foundation of Physics 201
High sensitivity tests of the Pauli Exclusion Principle with VIP2
The Pauli Exclusion Principle is one of the most fundamental rules of nature
and represents a pillar of modern physics. According to many observations the
Pauli Exclusion Principle must be extremely well fulfilled. Nevertheless,
numerous experimental investigations were performed to search for a small
violation of this principle. The VIP experiment at the Gran Sasso underground
laboratory searched for Pauli-forbidden X-ray transitions in copper atoms using
the Ramberg-Snow method and obtained the best limit so far. The follow-up
experiment VIP2 is designed to reach even higher sensitivity. It aims to
improve the limit by VIP by orders of magnitude. The experimental method,
comparison of different PEP tests based on different assumptions and the
developments for VIP2 are presented.Comment: 6 pages, 3 figures, Proceedings DISCRETE2014 Conferenc
Testing the Pauli Exclusion Principle for Electrons
One of the fundamental rules of nature and a pillar in the foundation of
quantum theory and thus of modern physics is represented by the Pauli Exclusion
Principle. We know that this principle is extremely well fulfilled due to many
observations. Numerous experiments were performed to search for tiny violation
of this rule in various systems. The experiment VIP at the Gran Sasso
underground laboratory is searching for possible small violations of the Pauli
Exclusion Principle for electrons leading to forbidden X-ray transitions in
copper atoms. VIP is aiming at a test of the Pauli Exclusion Principle for
electrons with high accuracy, down to the level of 10 - 10,
thus improving the previous limit by 3-4 orders of magnitude. The experimental
method, results obtained so far and new developments within VIP2 (follow-up
experiment at Gran Sasso, in preparation) to further increase the precision by
2 orders of magnitude will be presented.Comment: Proceedings DISCRETE 2012-Third Symposium on Prospects in the Physics
of Discrete Symmetries, Lisbon, December 3-7, 201
Application of photon detectors in the VIP2 experiment to test the Pauli Exclusion Principle
The Pauli Exclusion Principle (PEP) was introduced by the austrian physicist
Wolfgang Pauli in 1925. Since then, several experiments have checked its
validity. From 2006 until 2010, the VIP (VIolation of the Pauli Principle)
experiment took data at the LNGS underground laboratory to test the PEP. This
experiment looked for electronic 2p to 1s transitions in copper, where 2
electrons are in the 1s state before the transition happens. These transitions
violate the PEP. The lack of detection of X-ray photons coming from these
transitions resulted in a preliminary upper limit for the violation of the PEP
of . Currently, the successor experiment VIP2 is under
preparation. The main improvements are, on one side, the use of Silicon Drift
Detectors (SDDs) as X-ray photon detectors. On the other side an active
shielding is implemented, which consists of plastic scintillator bars read by
Silicon Photomultipliers (SiPMs). The employment of these detectors will
improve the upper limit for the violation of the PEP by around 2 orders of
magnitude
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