Water Entry Cavity Dynamics

When a sphere or a stream of water hits the surface of a pool of water and enters a crater or air cavity often forms. This topic has been studied, both formally and informally, for a long time. This dissertation investigates four areas of water impact that are still poorly understood using high-speed photography. First, it examines a stream of droplets impacting on a pool of water, similar to a faucet drizzling into a full bucket. For these types of impacts we predict the depth, diameter, velocity, and shape of the cavities that the droplet stream forms. Second, it examines what occurs when a sphere impacts a pool of soapy water, such as a bubble bath or kitchen sink. The minimum velocity for a cavity to form decreases when soap is present. If the water has bubbles on the surface, the sphere will always form a cavity. Third, it examines how different coatings on a sphere (car wax, etc.) affect whether the sphere forms a cavity, and it shows how the coating affect the shape of that cavity. Fourth, when objects impact a water surface they experience a large force, which many people have noticed when participating in cliff jumping, high diving, and belly flop competitions. We show that the force of impact can be reduced by 75% simply by allowing a mass of water to impact in front of the object

Water Entry of a Simple Harmonic Oscillator

When a blunt body impacts an air-water interface, large hydrodynamic forces often arise, a phenomenon many of us have unfortunately experienced in a failed dive or "belly flop." Beyond assessing risk to biological divers, an understanding and methods for remediation of such slamming forces are critical to the design of numerous engineered naval and aerospace structures. Herein we systematically investigate the role of impactor elasticity on the resultant structural loads in perhaps the simplest possible scenario: the water entry of a simple harmonic oscillator. Contrary to conventional intuition, we find that "softening" the impactor does not always reduce the peak impact force, but may also increase the force as compared to a fully rigid counterpart. Through our combined experimental and theoretical investigation, we demonstrate that the transition from force reduction to force amplification is delineated by a critical "hydroelastic" factor that relates the hydrodynamic and elastic timescales of the problem

Estrogen receptor transcription and transactivation: Basic aspects of estrogen action

Estrogen signaling has turned out to be much more complex and exciting than previously thought; the paradigm shift in our understanding of estrogen action came in 1996, when the presence of a new estrogen receptor (ER), ERβ, was reported. An intricate interplay between the classical ERα and the novel ERβ is of paramount importance for the final biological effect of estrogen in different target cells

Combined analysis of eIF4E and 4E-binding protein expression predicts breast cancer survival and estimates eIF4E activity

Increased eukaryotic translation initiation factor 4E (eIF4E) expression occurs in many cancers, and makes fundamental contributions to carcinogenesis by stimulating the expression of cancer-related genes at post-transcriptional levels. This key role is highlighted by the facts that eIF4E levels can predict prognosis, and that eIF4E is an established therapeutic target. However, eIF4E activity is a complex function of expression levels and phosphorylation statuses of eIF4E and eIF4E-binding proteins (4E-BPs). Our hypothesis was that the combined analyses of these pathway components would allow insights into eIF4E activity and its influence on cancer. We have determined expression levels of eIF4E, 4E-BP1, 4E-BP2 and phosphorylated 4E-BP1 within 424 breast tumours, and have carried out analyses to combine these and relate the product to patient survival, in order to estimate eIF4E activity. We show that this analysis gives greater prognostic insights than that of eIF4E alone. We show that eIF4E and 4E-BP expression are positively associated, and that 4E-BP2 has a stronger influence on cancer behaviour than 4E-BP1. Finally, we examine eIF4E, estimated eIF4E activity, and phosphorylated 4E-BP1 as potential predictive biomarkers for eIF4E-targeted therapies, and show that each determines selection of different patient groups. We conclude that eIF4E's influence on cancer survival is modulated substantially by 4E-BPs, and that combined pathway analyses can estimate functional eIF4E