5 research outputs found
Methods for the automatic recording of bird calls and songs in field ornithology
Der gegenwärtige Kenntnisstand über automatisierte Methoden
zur akustischen Erfassung von Rufen und Gesängen von
Vögeln wird dargelegt. Die Grundlage für eine automatisierte
Erfassung bilden Langzeitaufzeichnungen. Es wird der
Frage nachgegangen, inwiefern Tonaufzeichnungen für eine
qualitative und auch quantitative Analyse von Vogelbeständen
geeignet sind. Spezielles Augenmerk wird autonomen Aufzeichnungsmethoden
und der Auswertung von Langzeitaufzeichnungen
unter Nutzung von Algorithmen der akustischen
Mustererkennung gewidmet. Sinnvolle Einsatzszenarien für
automatisierte Methoden im Rahmen avifaunistischer Feldforschung
sind die Erfassung des nächtlichen Vogelzuges, die
Erfassung nachtaktiver Brutvogelarten und die Datenerhebung
in Kernzonen von Schutzgebieten.This review presents our current knowledge on automated methods for acoustic recording of calls and songs of birds. Acoustic
long-term recordings can serve as a basis for an automated bird census. We stress the question of whether sound recordings
are suitable for qualitative and quantitative analysis of bird populations. Special attention is devoted to autonomous recording
methods and the evaluation of long-term recordings by use of acoustic pattern recognition algorithms. Realistic scenarios for
the use of automated methods in field ornithology we see in the investigation of nocturnal bird migration, the census of
nocturnal bird species, and data collection in core areas of nature reserves
Compact and user-friendly ultrasound acquisition systems optimized for field recording
A compact and rugged hardware interface for laptop-based ultrasound recording in the field has been developed. The versatile devices overcome the limitations and drawbacks of previously available solutions. The accompanying recording software enables flexible hard disk recording for both manual and automated operation
Point-of-care testing system for digital single cell detection of MRSA directly from nasal swabs
We present an automated point-of-care testing (POCT) system for rapid detection of species- and resistance markers in methicillin-resistant Staphylococcus aureus (MRSA) at the level of single cells, directly from nasal swab samples. Our novel system allows clear differentiation between MRSA, methicillin-sensitive S. aureus (MSSA) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS), which is not the case for currently used real-time quantitative PCR based systems. On top, the novel approach outcompetes the culture-based methods in terms of its short time-to-result (1 h vs. up to 60 h) and reduces manual labor. The walk-away test is fully automated on the centrifugal microfluidic LabDisk platform. The LabDisk cartridge comprises the unit operations swab-uptake, reagent pre-storage, distribution of the sample into 20 000 droplets, specific enzymatic lysis of Staphylococcus spp. and recombinase polymerase amplification (RPA) of species (vicK) – and resistance (mecA) -markers. LabDisk actuation, incubation and multi-channel fluorescence detection is demonstrated with a clinical isolate and spiked nasal swab samples down to a limit of detection (LOD) of 3 ± 0.3 CFU μl−1 for MRSA. The novel approach of the digital single cell detection is suggested to improve hospital admission screening, timely decision making, and goal-oriented antibiotic therapy. The implementation of a higher degree of multiplexing is required to translate the results into clinical practice. © 2020 The Royal Society of Chemistry.ISSN:1473-0197ISSN:1473-018