Vertically-Aligned Functionalized Silicon Micropillars for 3D Culture of Human Pluripotent Stem Cell-Derived Cortical Progenitors

Silicon is a promising material for tissue engineering since it allows to produce micropatterned scaffolding structures resembling biological tissues. Using specific fabrication methods, it is possible to build aligned 3D network-like structures. In the present study, we exploited vertically-aligned silicon micropillar arrays as culture systems for human iPSC-derived cortical progenitors. In particular, our aim was to mimic the radially-oriented cortical radial glia fibres that during embryonic development play key roles in controlling the expansion, radial migration and differentiation of cortical progenitors, which are, in turn, pivotal to the establishment of the correct multilayered cerebral cortex structure. Here we show that silicon vertical micropillar arrays efficiently promote expansion and stemness preservation of human cortical progenitors when compared to standard monolayer growth conditions. Furthermore, the vertically-oriented micropillars allow the radial migration distinctive of cortical progenitors in vivo. These results indicate that vertical silicon micropillar arrays can offer an optimal system for human cortical progenitors' growth and migration. Furthermore, similar structures present an attractive platform for cortical tissue engineering

The Green Energy Audit, a new procedure for the sustainable auditing of existing buildings integrated with the LEED Protocols

Energy audits of buildings are the most effective tool to promote energy retrofitting measures for existing buildings, which are major consumers of energy in cities. Energy audits have multiple goals, including reducing energy consumption, managing costs, and environmental impact. The methodology of Green Energy Audit proposed defines an approach that is somewhat different from the traditional one. The added value lies in the word “green”, a word that refers to and summarises a common concept: sustainability. The proposed method is not intended to be a new energy auditing procedure, but rather a new and more modern interpretation of the classic methodology. The sustainability achieved by applying a retrofit measure is assessed with reference to the Leadership in Energy and Environmental Design Protocol. The Green Energy Audit integrates two strategic elements, energy and environment, by mixing the energy audit and LEED methodologies. This synergy strengthens the role of the classic energy audit by providing a method that not only optimise the energy performance of existing buildings but also achieve a green retrofit of buildings, making buildings, and so future cities, more sustainable. A case study of the application of this method is discussed in this paper

Immunogenicity and safety of three commercial influenza vaccines in institutionalized elderly

Influenza is a leading cause of morbidity and mortality in elderly people. This prospective, observed-blind, randomized, multicenter trial compares the immunogenicity and safety of three influenza vaccines in a sample of 635 elderly residents of four nursing homes in Milano (Italy). All vaccines were well tolerated: no serious adverse events were recorded, and a small number (9 subjects) of local and systemic reactions were observed. Twenty-nine oropharyngeal swabs were taken during the season from ILI (influenza-like illness) patients, none of whom was positive for influenza and other respiratory viruses. Immunogenicity was evaluated in a subgroup of 111 subjects with blood samples obtained just before vaccination and after 4 and 12 weeks. The adjuvanted vaccines, subunit vaccine with MF59 (a-SUV) and virosome subunit vaccine (v-SUV), induced a higher antibody response than whole virus vaccine (WVV). There was no significant difference between groups that received a-SUV and v-SUV, but the a-SUV group had higher values of geometric mean titres than the v-SUV group for H1N1 and B influenza strains. These findings suggest that influenza vaccination is effective, and they underscore the importance of vaccination programs for institutionalized elderly. Further studies are needed to compare other adjuvanted vaccines in order to define their different properties