On the Ironic Specimen of the Unicorn Horn in Enlightened Cabinets

This essay takes a material culture approach to the fate of the unicorn, that ultimate symbol of irrationality and credulity, in the natural history collection of the age of Enlightenment. Exploring the interplay between unicorn horns, narwhals, rhinos and other kinds of horn present in the eighteenth-century French collection, it shows that in fact unicorns never disappeared from the cabinet, but rather presided over new narratives of what Enlightenment was about. Further, it argues that this change in the status of unicorns was associated with changing patterns of the global whaling industry, which made narwhal horns widely available to Europeans, and the narwhal into a natural historical object. What real objects could, or could not, be represented in the collection as specimens had an important bearing upon the credibility of animal kinds outside the space of the cabinet, yet within that space, the juxtaposition and financial value of specimens produced important narratives of the relationship between horn specimens and natural species like rhinos and narwhals existing in the real world—species which never completely shed their fictive character, like the unicorn itself

Opium, Experimentation, and Alterity in France

AbstractThe effects and dangers of opium were subject to intense scientific scrutiny and experimentation in Paris in the decades around 1700, as rival networks of healers contended for commercial advantage over the compound drugs that contained it. Opium, widely consumed in the Ottoman empire, became a subject of European scientific interest in an attempt to render it safe, agreeable, and beneficial for European bodies. Apothecaries sought to resurrect an ancient drug and infuse it with new life in the laboratory; physicians conducted chemical experiments upon it. Yet it was hard to reach agreement as to opium's harmful or beneficial effects; some aspects of its nature proved impossible to ‘domesticate’ in the same way as other exotic drugs like coffee or tea, or even cinchona. I argue that only by investigating the discrete networks which sold and experimented upon opium can the historian account for the ways in which this drug generated social, political, and financial capital for experimenters as it circulated throughout society.Leverhulme Trust Research Project RPG-2014 'Selling the Exotic in Paris and Versailles

Mainstreaming gender in development policy : a comparative analysis of Tamil Nadu and Andhra Pradesh, India

EThOS - Electronic Theses Online ServiceGBUnited Kingdo

The Wales Cancer Bank (WCB)

The Wales Cancer Bank (WCB) was established in 2004 and consents patients in Wales, UK with a known or suspected cancer diagnosis to donate biosamples for future research. The resource is open access to all researchers working in cancer research, regardless of geographic location or employment sector. To December 2017, just over 13,500 patients have donated samples across a variety of tumour types. Tumour and adjacent normal tissue and blood samples are routinely collected, stored and processed to standardised protocols. Bespoke collections for unique samples such as urine or ascites are also available. Pathology data, clinical data including treatment and outcome data and selected molecular data is also available. Funding statement: The WCB is currently funded as part of the Wales Cancer Research Centre by Health and Care Research Wales. Funding is also received from Velindre Charitable funds and Cancer Research UK’s Stratified Medicine Programme. Previous funding has also been received from Cancer Research Wales

Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts via toll-like receptor 4.

AIM: To investigate the effect of Tenascin C (TNC) on the expression of pro-inflammatory cytokines and matrix metalloproteinases in human cardiac myofibroblasts (CMF). METHODS: CMF were isolated and cultured from patients undergoing coronary artery bypass grafting. Cultured cells were treated with either TNC (0.1 μmol/L, 24 h) or a recombinant protein corresponding to different domains of the TNC protein; fibrinogen-like globe (FBG) and fibronectin type III-like repeats (TNIII 5-7) (both 1 μmol/L, 24 h). The expression of the pro-inflammatory cytokines; interleukin (IL)-6, IL-1β, TNFα and the matrix metalloproteinases; MMPs (MMP1, 2, 3, 9, 10, MT1-MMP) was assessed using real time RT-PCR and western blot analysis. RESULTS: TNC increased both IL-6 and MMP3 (P < 0.01) mRNA levels in cultured human CMF but had no significant effect on the other markers studied. The increase in IL-6 mRNA expression was mirrored by an increase in protein secretion as assessed by enzyme-linked immunosorbant assay (P < 0.01). Treating CMF with the recombinant protein FBG increased IL-6 mRNA and protein (P < 0.01) whereas the recombinant protein TNIII 5-7 had no effect. Neither FBG nor TNIII 5-7 had any significant effect on MMP3 expression. The expression of toll-like receptor 4 (TLR4) in human CMF was confirmed by real time RT-PCR, western blot and immunohistochemistry. Pre-incubation of cells with TLR4 neutralising antisera attenuated the effect of both TNC and FBG on IL-6 mRNA and protein expression. CONCLUSION: TNC up-regulates IL-6 expression in human CMF, an effect mediated through the FBG domain of TNC and via the TLR4 receptor

Prostaglandin E2-mediated adenosinergic effects on CD14+ cells: self-amplifying immunosuppression in cancer

CD39 and CD73 are surface-expressed ectonucleotidases that hydrolyze ATP in a highly regulated, serial manner into ADP, AMP and adenosine. The end product, adenosine, has both tumor-promoting and immunosuppressive effects. The aim of this study was to determine CD73 expression on immune cells in pleural effusion (PE) in order to have a better understanding of the immune environment in mesothelioma. PE- or blood-derived CD14+ cells of mesothelioma patients and healthy donors were analyzed by flow cytometry for the expression of CD39 and CD73. CD73-induction was studied by exposure of CD14+ cells to the soluble fraction of PE (sPE), while the signaling mechanism, responsible for CD73 induction, by phosphoflow cytometry and receptor-inhibition studies. We observed CD73 expression on CD14+ cells in PE but not peripheral blood of mesothelioma patients or healthy donors. CD73 expression was inducible on CD14+ cells with sPE, cyclic-AMP (cAMP)-inducers (forskolin and prostaglandin-E2 (PGE2)) and adenosine. Inhibition of PGE2 receptors or adenosine A2 receptors blocked CD73-induction by sPE. sPE treatment triggered protein kinase A and p38 activation. However, signal-transducer and activator of transcription 3 (STAT3)-blocking led to enhanced CD73 expression, demonstrating a hitherto unknown negative control of purinergic signaling by STAT3 in CD14+ cells. TNFα production by CD73+ CD14+ cells was significantly impaired in the presence of AMP, confirming immunosuppressive function. Taken together, CD73 expression can be induced by PGE2, cAMP or adenosine on human CD14+ cells. We suggest that targeting this autocrine loop is a valid therapeutic approach in mesothelioma that may also enhance immunotherap

Proteomics analysis of vesicles isolated from plasma and urine of prostate cancer patients using a multiplex, aptamer-based protein array

Proteomics analysis of biofluid-derived vesicles holds enormous potential for discovering non-invasive disease markers. Obtaining vesicles of sufficient quality and quantity for profiling studies has, however, been a major problem, as samples are often replete with co-isolated material that can interfere with the identification of genuine low abundance, vesicle components. Here, we used a combination of ultracentrifugation and size-exclusion chromatography to isolate and analyse vesicles of plasma or urine origin. We describe a sample-handling workflow that gives reproducible, quality vesicle isolations sufficient for subsequent protein profiling. Using a semi-quantitative aptamer-based protein array, we identified around 1,000 proteins, of which almost 400 were present at comparable quantities in plasma versus urine vesicles. Significant differences were, however, apparent with elements like HSP90, integrin αVβ5 and Contactin-1 more prevalent in urinary vesicles, while hepatocyte growth factor activator, prostate-specific antigen–antichymotrypsin complex and many others were more abundant in plasma vesicles. This was also applied to a small set of specimens collected from men with metastatic prostate cancer, highlighting several proteins with the potential to indicate treatment refractory disease. The study provides a practical platform for furthering protein profiling of vesicles in prostate cancer, and, hopefully, many other disease scenarios