The influence of systematic structure alterations on the photophysical properties and conjugation characteristics of asymmetric cyanine 5 dyes

Imaging- and therapeutic targets in neoplastic and musculoskeletal inflammatory diseas

Obtaining control of cell surface functionalizations via Pre-targeting and Supramolecular host guest interactions

The use of mammalian cells for therapeutic applications is finding its way into modern medicine. However, modification or “training” of cells to make them suitable for a specific application remains complex. By envisioning a chemical toolbox that enables specific, but straight-forward and generic cellular functionalization, we investigated how membrane-receptor (pre)targeting could be combined with supramolecular host-guest interactions based on β-cyclodextrin (CD) and adamantane (Ad). The feasibility of this approach was studied in cells with membranous overexpression of the chemokine receptor 4 (CXCR4). By combining specific targeting of CXCR4, using an adamantane (Ad)-functionalized Ac-TZ14011 peptide (guest; KD = 56 nM), with multivalent host molecules that entailed fluorescent β-CD-Poly(isobutylene-alt-maleic-anhydride)-polymers with different fluorescent colors and number of functionalities, host-guest cell-surface modifications could be studied in detail. A second set of Ad-functionalized entities enabled introduction of additional surface functionalities. In addition, the attraction between CD and Ad could be used to drive cell-cell interactions. Combined we have shown that supramolecular interactions, that are based on specific targeting of an overexpressed membrane- receptor, allow specific and stable, yet reversible, surface functionalization of viable cells and how this approach can be used to influence the interaction between cells and their surroundings.</p

Tracers for Fluorescence-Guided Surgery: How Elongation of the Polymethine Chain in Cyanine Dyes Alters the Pharmacokinetics of a Dual-Modality c[RGDyK] Tracer

Imaging- and therapeutic targets in neoplastic and musculoskeletal inflammatory diseas

Supramolecular host-guest chemistry for applications in theranostics

The possibility of employing supramolecular interactions to solve theranostics challenges were discussed in this work. The possibility of utilizing supramolecular host–guest chemistry between β-Cyclodextrin and Adamantane to drive cell functionalization and cell–cell interactions in an in vitro environment was explored; the resulting pre-targeting set-up was further tested in vivo for liver radioembolization procedures. Furthermore, the self-assembly properties of ferritin were investigated to make a step towards the creation of new chemical entities that can be applied in precision therapy.European Research Council Grant No. 2012-306890LUMC / Geneeskund

Computational and experimental data on electrostatic density and stacking tendency of asymmetric cyanine 5 dyes

Far-red dyes such as cyanine 5 (Cy5) are gaining interest in (bio)medical diagnostics as they have promising features in terms of stability and brightness. Here, the electrostatic density and stacking tendency in different solvents of nine systematically altered asymmetrical Cy5 dyes are reported. In addition to this, the influence of molecular alterations on the vibronic coupling was reported. The data presented supplement to the recent study “The influence of systematic structure alterations on the photophysical properties and conjugation characteristics of asymmetric cyanine 5 dyes” (Spa et al., 2018). Keywords: Fluorescence, Cyanine, Stacking, Electrostatic densit

The influence of structural alterations on asymmetric cyanine 5 dyes on the photophysical properties and protein conjugation characteristics

Imaging- and therapeutic targets in neoplastic and musculoskeletal inflammatory diseas

In vivo stability of supramolecular host-guest complexes monitored by dual-isotope multiplexing in a pre-targeting model of experimental liver radioembolization

Host-parasite interactio

Fluorescent Lectins for Local in Vivo Visualization of Peripheral Nerves

Imaging- and therapeutic targets in neoplastic and musculoskeletal inflammatory diseas

How the chemical structure of cyanine dyes influences the in vivo properties of receptor targeted tracers - exemplified using hybrid cRGDyK-analogues

Imaging- and therapeutic targets in neoplastic and musculoskeletal inflammatory diseas

Multimodal Tracking of Controlled Staphylococcus aureus Infections in Mice

Imaging- and therapeutic targets in neoplastic and musculoskeletal inflammatory diseas