Comparison of polysomal association between 0-2 hour, 4-6 hour and 8-10 hour old embryos

<p><b>Copyright information:</b></p><p>Taken from "Global analyses of mRNA translational control during early embryogenesis"</p><p>Genome Biology 2007;8(4):R63-R63.</p><p>Published online 22 Apr 2007</p><p>PMCID:PMC1896012.</p><p></p> Smoothed histograms (density plots) of Logits for individual transcript species from 0-2 hour, 4-6 hour and 8-10 hour old embryos were determined. x-axis, Logit, defined as log {percent(6-12)/percent(1-5)}; y-axis, density of Logit. The left panel includes all the probe sets, the middle panel includes the probe sets with the top 50% signal intensity and the right panel includes only the probe sets with the top 25% intensity. Smoothed histograms (density plots) of Logits of the genes encoding cytoplasmic large ribosomal proteins (GO0005842, 56 genes, left), cytoplasmic small ribosomal proteins (GO0005943, 48 genes, middle) and transcription activator (GO0016563, 35 genes, right). The arrows point in the direction of development

Percentile polysomal profiles of representative mRNAs whose polysomal association is highly regulated during early embryonic development

<p><b>Copyright information:</b></p><p>Taken from "Global analyses of mRNA translational control during early embryogenesis"</p><p>Genome Biology 2007;8(4):R63-R63.</p><p>Published online 22 Apr 2007</p><p>PMCID:PMC1896012.</p><p></p> Translational regulation between 0-2 hours and 4-6 hours. Duplicate array experiments prepared from independently fractionated RNA samples are shown individually. The first point is the sum of mRNAs from the first five non-polysomal fractions (fractions 1-5). Fen1 is up-regulated after the first two hours while PNGase is down-regulated. Their relative mRNA abundance is plotted in the far right panel. The abundance is shown as a Log2 scale (y-axis), which is determined by the signal intensity on the GeneChips and normalized as described in Materials and methods. Translational regulation between 4-6 hours and 8-10 hours. Fat-spondin is up-regulated between 4-6 hours and 8-10 hours while ApepP is down-regulated. The right panel illustrates the relative mRNA abundance at each time period

Percentile polysomal profiles of several known subcellularly localized transcripts

<p><b>Copyright information:</b></p><p>Taken from "Global analyses of mRNA translational control during early embryogenesis"</p><p>Genome Biology 2007;8(4):R63-R63.</p><p>Published online 22 Apr 2007</p><p>PMCID:PMC1896012.</p><p></p> Percentile polysomal profiles of , and from 0-2 hour old embryos. Percentile polysomal profiles of caudal mRNAs and hunchback mRNAs at 0-2 hours, 4-6 hours and 8-10 hours. Two sets of microarrays prepared from independent gradient-fractionated RNAs are shown as the open and closed circles individually. The right panel illustrates their relative mRNA abundance (y-axis) at each time period (x-axis)

Representative ultraviolet absorbance profile for embryonic RNA fractionated by 20% to 50% sucrose density gradients

<p><b>Copyright information:</b></p><p>Taken from "Global analyses of mRNA translational control during early embryogenesis"</p><p>Genome Biology 2007;8(4):R63-R63.</p><p>Published online 22 Apr 2007</p><p>PMCID:PMC1896012.</p><p></p> The 80S ribosomal subunit position is indicated. RNAs were purified from each fraction, electrophoresed on an agarose gel and visualized using ethidium bromide. The bands as shown are predominantly rRNAs. Equal amounts of an RNA aliquot from the first five fractions were pooled for microarray analysis. RNAs from the other fractions were individually prepared for oligoarray analysis as indicated (see Materials and methods)

The ultraviolet absorbance profiles at OD 254 nm from 0-2 hour, 4-6 hour and 8-10 hour old embryos

<p><b>Copyright information:</b></p><p>Taken from "Global analyses of mRNA translational control during early embryogenesis"</p><p>Genome Biology 2007;8(4):R63-R63.</p><p>Published online 22 Apr 2007</p><p>PMCID:PMC1896012.</p><p></p

La Petite presse : journal quotidien... / [rédacteur en chef : Balathier Bragelonne]

04 juillet 18701870/07/04 (A5,N1537)

Visualization 5: Optical clearing based cellular-level 3D visualization of intact lymph node cortex

Z-stack movie of metastasized LN at 10 days after the tumor inoculation Originally published in Biomedical Optics Express on 01 October 2015 (boe-6-10-4154

Intravital longitudinal wide-area imaging of dynamic bone marrow engraftment and multilineage differentiation through nuclear-cytoplasmic labeling

<div><p>Bone marrow is a vital tissue that produces the majority of erythrocytes, thrombocytes, and immune cells. Bone marrow transplantation (BMT) has been widely performed in patients with blood disorders and cancers. However, the cellular-level behaviors of the transplanted bone marrow cells over wide-areas of the host bone marrow after the BMT are not fully understood yet. In this work, we performed a longitudinal wide-area cellular-level observation of the calvarial bone marrow after the BMT <i>in vivo</i>. Using a H2B-GFP/β-actin-DsRed double-transgenic mouse model as a donor, a subcellular-level nuclear-cytoplasmic visualization of the transplanted bone marrow cells was achieved, which enabled a direct <i>in vivo</i> dynamic monitoring of the distribution and proliferation of the transplanted bone marrow cells. The same spots in the wide-area of the calvarial bone marrow were repeatedly identified using fluorescently labeled vasculature as a distinct landmark. It revealed various dynamic cellular-level behaviors of the transplanted BM cells in early stage such as cluster formation, migration, and active proliferation <i>in vivo</i>.</p></div

Dynamic time-lapse nuclear-cytoplasmic visualization of the transplanted BM cell.

<p>Multi-position time-lapse imaging of the calvarial bone marrow at 6 minute intervals was performed 3 days after the BMT. (A) Proliferation events observed during the 5 hour time-lapse imaging (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0187660#pone.0187660.s004" target="_blank">S1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0187660#pone.0187660.s005" target="_blank">S2</a> Movies). (B, C) Representative time-lapse image sequences showing (B) proliferation (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0187660#pone.0187660.s006" target="_blank">S3</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0187660#pone.0187660.s007" target="_blank">S4</a> Movies) or (C) migration of the transplanted BM cell (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0187660#pone.0187660.s008" target="_blank">S5 Movie</a>). Scale bar is 10 μm.</p

Quantitative imaging analysis of transplanted BM cells.

<p>Quantitative imaging analysis was performed by using the large mosaic images of calvarial BM of three BMT mouse models. (A) The ratio of the total area occupied by actin-DsRed expressing cells in the calvalrial BM. (n = 3). (B) The ratio of the total cell area and the fractional ratio occupied by three groups comprising single BM cells or their clusters categorized by their size: < 500 μm²; 500–5,000 μm²; > 5,000 μm². (C) The number of cell clusters in each group. (n = 3).</p