6 research outputs found
Identification of a potent herbal molecule for the treatment of breast cancer
<p>Abstract</p> <p>Background</p> <p>Breast cancer (BCa)-related mortality still remains the second leading cause of cancer-related deaths worldwide. Patients with BCa have increasingly shown resistance and high toxicity to current chemotherapeutic drugs for which identification of novel targeted therapies are required.</p> <p>Methods</p> <p>To determine the effect of PDBD on BCa cells, estrogen-receptor positive (ER<sup>+</sup>)-MCF-7 and estrogen-receptor negative (ER<sup>-</sup>)-MDA 231 cells were treated with PDBD and the cell viability, apoptotic, cell cycle, Western blot and Promoter assays were performed.</p> <p>Results</p> <p>PDBD inhibits cell viability of ER<sup>+ </sup>and ER<sup>- </sup>BCa cells by inducing apoptosis without causing significant toxicity in normal breast epithelial cells. While dissecting the mechanism of action of PDBD on BCa, we found that PDBD inhibits Akt signaling and its downstream targets such as NF-κB activation, IAP proteins and Bcl-2 expression. On the other hand, activation of JNK/p38 MAPK-mediated pro-apoptotic signaling was observed in both ER<sup>+ </sup>and ER<sup>- </sup>BCa cells.</p> <p>Conclusion</p> <p>These findings suggest that PDBD may have wide therapeutic application in the treatment of BCa.</p
Anticonvulsant activity of semicarbazone derivatives of Mannich bases
2657-2661A series of semicarbazones of semicarbazide/p-chlorophenyl
semicarbazide and Mannich bases of acetophenone/p-chloroacetophenone has
been synthesized and their anticonvulsant activity screened against MES and scPTZ
test. p-Chlorophenyl semicarbazone of N,N-dimethylaminopropiophenone has
been found to be the most active in all these tests
The antiepileptic effect of Centella asiatica on the activities of Na+/K+, Mg2+ and Ca2+-ATPases in rat brain during pentylenetetrazol–induced epilepsy
Background: To study the anticonvulsant effect of different extracts
of Centella asiatica (CA) in male albino rats with reference to Na +
/K + , Mg 2+ and Ca 2+ -ATPase activities. Materials and Methods: Male
Wistar rats (150±25 g b.w.) were divided into seven groups of six
each i.e. (a) control rats treated with saline, (b) pentylenetetrazol
(PTZ)-induced epileptic group (60 mg/kg, i.p.), (c) epileptic group
pretreated with n-hexane extract (n-HE), (d) epileptic group pretreated
with chloroform extract (CE), (e) epileptic group pretreated with ethyl
acetate extract (EAE), (f) epileptic group pretreated with n-butanol
extract (n-BE), and (g) epileptic group pretreated with aqueous extract
(AE). Results: The activities of three ATPases were decreased in
different regions of brain during PTZ-induced epilepsy and were
increased in epileptic rats pretreated with different extracts of CA
except AE. Conclusion: The extracts of C. asiatica, except AE, possess
anticonvulsant and neuroprotective activity and thus can be used for
effective management in treatment of epileptic seizures