Immune and inflammatory responses to Leishmania amazonensis isolated from different clinical forms of human leishmaniasis in CBA mice

Leishmania amazonensis causes different diseases depending on the host and parasitic virulence factors. In this study, CBA mice were infected with L. amazonensis isolates from patients with localized (Ba125), diffuse cutaneous (Ba276) or visceral leishmaniasis (Ba109). Mice infected with Ba125 and Ba276 progressed rapidly and lesions displayed an infiltrate rich in parasitized macrophages and were necrotic and ulcerated. Ba109 induced smaller lesions and a mixed inflammatory infiltrate without necrosis or ulceration. Ba109 induced an insidious disease with lower parasite load in CBA mice, similar to human disease. Levels of IFN-γ, IL-4 and IL-10 did not differ among the groups. Because all groups were unable to control the infection, expression of IL-4 associated with low production of IFN-γ in the early phase of infection may account for susceptibility, but others factors may contribute to the differences observed in inflammatory responses and infection progression. Evaluation of some parasitic virulence factors revealed that Ba276 exhibits higher ecto-ADPase and 5'-nucleotidase activities compared to the Ba109 and Ba125 strains. Both Ba276 and Ba125 had higher arginase activity in comparison to Ba109. Finally, these data suggest that the differences in enzyme activities among parasites can account for differences in host inflammatory responses and infection progression

1,8-cineole protects against liver failure in an in-vivo murine model of endotoxemic shock

Freitas, Luiz Antônio Rodrigues de “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2017-08-11T17:34:03Z No. of bitstreams: 1 Santos FA 1,8-cineole protects....pdf: 313163 bytes, checksum: 4b7a5a588d0cf5796c69e4c36104b90e (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2017-08-11T17:51:35Z (GMT) No. of bitstreams: 1 Santos FA 1,8-cineole protects....pdf: 313163 bytes, checksum: 4b7a5a588d0cf5796c69e4c36104b90e (MD5)Made available in DSpace on 2017-08-11T17:51:35Z (GMT). No. of bitstreams: 1 Santos FA 1,8-cineole protects....pdf: 313163 bytes, checksum: 4b7a5a588d0cf5796c69e4c36104b90e (MD5) Previous issue date: 2001Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, 300870/98-1).Federal University of Ceará. Department of Physiology and Pharmacology. Fortaleza, CE, BrasilFederal University of Ceará. Department of Physiology and Pharmacology. Fortaleza, CE, BrasilFederal University of Ceará. Department of Physiology and Pharmacology. Fortaleza, CE, BrasilFederal University of Ceará. Department of Physiology and Pharmacology. Fortaleza, CE, BrasilFederal University of Ceará. Department of Pathology and Legal Medicine. Fortaleza,Federal University of Ceará. Department of Pathology and Legal Medicine. Fortaleza,Fundação Oswaldo Cruz. Laboratório de Patologia e Biologia Celular. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Laboratório de Patologia e Biologia Celular. Rio de Janeiro, RJ, BrasilThe effects of 1,8-cineole on D-galactosamine/lipopolysaccharide (GalN/LPS)-induced shock model of liver injury was investigated in mice. The co-administration of GalN (700 mg kg−1, i.p.) and LPS (5 lgkg−1, i.p.) greatly elevated serum concentrations of tumour necrosis factor-a (TNFa), alanine aminotransferase and aspartate aminotransferase, and induced massive hepatic necrosis and lethality in 100% of control mice. Pretreatment with 1,8-cineole (400 mg kg−1, p.o.) and dexamethasone (1 mgkg−1, s.c.), 60 min before GalN/LPS, offered complete protection (100%) against the lethal shock and acute elevation in serum TNF-a and serum transaminases. Hepatic necrosis induced by GalN/LPS was also greatly reduced by both 1,8-cineole and dexamethasone treatment. The results indicate that 1,8-cineole protects mice against GalN/LPSinduced liver injury through the inhibition of TNF-a production, and suggest that 1,8-cineole may be a promising agent to combat septic-shock-associated pathologies