11 research outputs found
Liposomal antagomiR-155-5p restores anti-inflammatory macrophages and improves arthritis in preclinical models of rheumatoid arthritis
Objective: We previously reported an increased expression of microRNAâ155 (miRâ155) in the blood monocytes of patients with rheumatoid arthritis (RA) that could be responsible for impaired monocyte polarization to antiâinflammatory M2âlike macrophages. In this study, we employed two preclinical models of RA, collagenâinduced arthritis and K/BxN serum transfer arthritis, to examine the therapeutic potential of antagomiRâ155â5p entrapped within PEGylated (polyethylene glycol [PEG]) liposomes in resolution of arthritis and repolarization of monocytes towards the antiâinflammatory M2 phenotype. Methods: AntagomiRâ155â5p or antagomiRâcontrol were encapsulated in PEG liposomes of 100 nm in size and â10 mV in zeta potential with high antagomiR loading efficiency (above 80%). Mice were injected intravenously with 1.5 nmol/100 ÎŒL PEG liposomes containing antagomiRâ155â5p or control after the induction of arthritis. Results: We demonstrated the biodistribution of fluorescently tagged PEG liposomes to inflamed joints one hour after the injection of fluorescently tagged PEG liposomes, as well as the liver's subsequent accumulation after 48 hours, indicative of hepatic clearance, in mice with arthritis. The injection of PEG liposomes containing antagomiRâ155â5p decreased arthritis score and paw swelling compared with PEG liposomes containing antagomiRâcontrol or the systemic delivery of free antagomiRâ155â5p. Moreover, treatment with PEG liposomes containing antagomiRâ155â5p led to the restoration of bone marrow monocyte defects in antiâinflammatory macrophage differentiation without any significant functional change in other immune cells, including splenic B and T cells. Conclusion: The injection of antagomiRâ155â5p encapsulated in PEG liposomes allows the delivery of small RNA to monocytes and macrophages and reduces joint inflammation in murine models of RA, providing a promising strategy in human disease.imag
Planification stratĂ©gique forestiĂšre - guide de bonnes pratiques pour les forĂȘts alpines
NEWFOR (NEW technologies for a better mountain FORest timber mobilization) is a research project funded by the alpine space programme. It brings together 14 institutes of the 6 countries of the alpine space with the aim of improving forest timber evaluation and mobilisation. The project considers the whole wood supply chain, from forests to wood yards, with a particular emphasis on new remote sensing technologies and geographical information systems. The report presents the major findings of the project and outlines some key issues for a better mobilisation of alpine timber ressoures