Expression of circadian clock genes and diurnal oscillations of key physiological events in response to AsV and AsIII in soybean plants

Soybean (Glycine max L.) is often cultivated in soils with a high content of arsenic (As). Since this greatly affects crop growth and yield, the response mechanisms underlying tolerance to As should be well understood. Circadian rhythms are known to play a central role in plant adaptation to stressful conditions. Therefore, our aim was to evaluate the effects of arseniate (AsV) and arsenite (AsIII) on the expression of key circadian clock genes (CG) and on the possible diurnal oscillation of physiological events induced by As in soybean plants. Leaves and roots were evaluated during light-dark phases at 4-h intervals. The changes caused by As in the expression profiles of all CGs analyzed depended on its chemical form and the plant organ involved. Overexpression of GmLCL1, GmTOC1, GmPRR9 and GmGI was observed in leaves mainly upon AsV-treatment, while underexpression of GmLCL1 and overexpression of GmPRR9, GmELF4 and GmGI occurred in roots, mainly upon AsIII-treatment. Moreover, As seemed to have an influence on the daily fluctuations observed in events triggered in response to the stress, such as stomatal aperture control, phytochelatin (PC) and glutathione (GSH) content, and total antioxidant, ascorbate (APx) and glutathione peroxidase (GPx) activities. In particular, the increase in the synthesis of PCs (the main As chelators) and the decrease in their precursor, GSH, were higher during the light phase, while APx and GPx activities increased during the dark phase in As-treated plants. The results suggest a link between the circadian clock and the response to As in soybean plants, since exposure to As modified CG expression and induced important changes in the diurnal oscillation of several As-response mechanisms. Circadian clock regulation, then, might play a vital role in the tolerance to As.Fil: Vezza, Mariana Elisa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Talano, Melina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentin

Proliferative glioblastoma cancer cells exhibit persisting temporal control of metabolism and display differential temporal drug susceptibility in chemotherapy

Even in immortalized cell lines, circadian clocks regulate physiological processes in a time-dependent manner, driving transcriptional and metabolic rhythms, the latter being able to persist without transcription. Circadian rhythm disruptions in modern life (shiftwork, jetlag, etc.) may lead to higher cancer risk. Here, we investigated whether the human glioblastoma T98G cells maintained quiescent or under proliferation keep a functional clock and whether cells display differential time responses to bortezomib chemotherapy. In arrested cultures, mRNAs for clock (Per1, Rev-erbα) and glycerophospholipid (GPL)-synthesizing enzyme genes, 32 P-GPL labeling, and enzyme activities exhibited circadian rhythmicity; oscillations were also found in the redox state/peroxiredoxin oxidation. In proliferating cells, rhythms of gene expression were lost or their periodicity shortened whereas the redox and GPL metabolisms continued to fluctuate with a similar periodicity as under arrest. Cell viability significantly changed over time after bortezomib treatment; however, this rhythmicity and the redox cycles were altered after Bmal1 knock-down, indicating cross-talk between the transcriptional and the metabolic oscillators. An intrinsic metabolic clock continues to function in proliferating cells, controlling diverse metabolisms and highlighting differential states of tumor suitability for more efficient, time-dependent chemotherapy when the redox state is high and GPL metabolism low.Fil: Wagner, Paula Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gorne, Lucas Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Salvador, Gabriela Alejandra. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Pasquaré, Susana Juana. Universidad Nacional del Sur; ArgentinaFil: Guido, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentin

A homemade laboratory applied to the phytoremediation of textile dyes by high school students in the context of COVID-19 pandemic

Biosphere contamination is a current environmental and social issue that must be included in the school programme. It is essential that students recognize their responsibility and be aware about the generation and reduction of environmental pollution. Dyes are usually used in school activities and discharged into household effluents, constituting potential toxic agents. To offer solutions to this problem, it was proposed to evaluate the ability of Lemna sp. plants for removing synthetic dyes. For this, activities that require accessible resources and minimum safety standards were designed, which could be carried out by students at home in the virtual education modality imposed by the COVID-19 pandemic. The practical experience integrated theoretical-practical contents from different disciplines, achieving a true interdisciplinary dialogue. The use of Lemna sp. allowed to obtain a fast and significant dye decolorization, demonstrating the importance of biodiversity in the attenuation of environmental changes due to anthropogenic activities. This experience allowed us to arrive at an interdisciplinary, significant and situated learning through the implementation of the scientific method.Fil: Vezza, Mariana Elisa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Instituto Provincial de Enseñanza Media Numero 42 Profesora Marcela Beatriz Moyano Coudert.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Antioxidant response of tobacco (Nicotiana tabacum) hairy roots after phenol treatment

Phenol is released to the environment from a wide variety of industrial effluents and it causes severe problems to human health and ecosystem. In the present study, we determined that Nicotiana tabacum hairy roots (HRs) double transgenic (DT) for two peroxidase genes (tpx1 and tpx2) showed higher phenol removal efficiency than wild type (WT) HRs after 120 h of phenol treatment at the expense of endogenous H 2O 2. Besides, to determine whether phenol could induce oxidative stress on tobacco HRs, we analyzed the antioxidant response, superoxide anion (O 2 -) localization and malondialdehyde (MDA) levels. Both HRs treated with phenol, showed significant increases in peroxidase (PX) activity mainly at the end of the assay (120 h) being PX activity from transgenic HRs 40% higher than that of WT HRs. Superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities showed significant increases from 24 to 120 h of phenol treatment. PX, SOD and APX isoforms were also analyzed and slight changes were observed only in PX patterns. Both HRs showed significant differences in total glutathione (TGSH) content during treatment, being higher in DT HRs than in WT HRs. At the end of the assay, a greater accumulation of O 2 - in different root zones was observed in WT and DT HRs. Moreover, phenol was able to increase the MDA levels in WT HRs from 48 to 120 h of the treatment, but no significant changes were observed in DT HRs. Results suggest that under these experimental conditions, DT HRs would be more tolerant to phenol than WT HRs.Fil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Medina, Maria Inés. Universidad Nacional de Río Cuarto; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentin

Impact of phenol on the glycerophospholipid turnover and potential role of circadian clock in the plant response against this pollutant in tobacco hairy roots

Glycerophospholipids (GPLs) from cell membranes (CM) are a proper source for the synthesis of lipid messengers able to activate signal pathways that will define the plant survival under changing and stressful environmental conditions. Little is known about how GPLs metabolism (GPLsM) is regulated and the effects of phenol treatment on GPLs composition. In this work, we studied the effects of phenol both on GPLs turnover and on the expression of GPLsM-related genes potentially regulated by the circadian clock, using tobacco hairy root cultures (HRC). Phenol decreased the total PC levels and increased PE, PG and CL levels in the dark phase. Different molecular species of PC and PE showed the same trend than the total PC and PE upon phenol treatment. Besides, significant differences in the expression of all studied genes related to GPLsM were found. NtCCT2 expression was affected at all analyzed times while NtPECT1 and NtAAPT1 showed similar expression patterns. NtCDS1, NtPGPS2 and NtCLS genes showed significant and differential expression profiles both in untreated and treated HRC. PECT1 and NtPGPS2 genes seem to conserve a circadian expression profile mainly in untreated HRC. However, phenol was able to modify the GPLs composition and the expression of genes related to GPLs synthesis. The GPLs modification could be explained by the up-regulation of NtPECT1, NtAAPT1 and NtCLS genes during the dark phase, suggesting for being a crucial moment for HRC to trigger an adaptive response against this organic pollutant.Fil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lucangioli, Silvia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Agostini, Elizabeth. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentin

Tobacco hairy root's peroxidases are rhythmically controlled by phenol exposure

Plants like almost all living organisms, have developed a biological clock or circadian clock (CC) capable of synchronizing and adjusting various metabolic and physiological processes at certain times of the day and in a period of 24 h. This endogenous timekeeping is able to predict the environmental changes providing adaptive advantages against stressful conditions. Therefore, the aim of this work was to analyze the possible link between metabolism of xenobiotic compounds (MXC) and the CC. Synchronized Nicotiana tabacum hairy roots (HRs) were used as a validated plant model system, and peroxidases (PODs), key enzymes of the phase I in the MCX, were evaluated after phenol treatment. Two POD genes were selected and their temporal expression profiles as well as the total POD activity were analyzed in order to find circadian oscillations either under control conditions or phenol treatment. It was demonstrated that these PODs genes showed oscillatory profiles with an ultradian period (period length shorter than the circadian period), and preserving the same phases and expression peaks still under phenol treatment. The total PODs activity showed also a marked oscillatory behavior mainly in phenol-treated HRs with the highest levels at ZT23. Untreated HRs showed decrease and increase in the intensity of some basic isoforms at light and dark phase, respectively, while in phenol- treated HRs, an increase in the intensity of almost all isoforms was observed, mainly during the dark phase, being coincident with the high PODs activity detected at ZT23. The periodic analysis determined an ultradian period either in total POD activity or in the POD activity of isoform VI, being 18.7 and 15.3 h, respectively. Curiously, in phenol treated HRs, the period length of total POD activity was longer than in untreated HRs, suggesting that phenol could induce a marked oscillatory behavior in the POD activity with better performance during the dark phase, which explain the higher phenol removal efficiencies at ZT23. These findings showed novel information about the performance of PODs, which would be rhythmically controlled at biochemical level, by phenol exposure.Fil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Ronchi, Hebe Luz. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Monjes, Natalia Maribel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; ArgentinaFil: Agostini, Elizabeth. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentin

Is the PLC pathway involved in the response to phenol treatment in tobacco hairy roots?

Phospholipids and phospholipases play important roles in several cellular processes and responses to adverse growth conditions. However, the mechanism of action of phosphatidylinositol-specific phospholipase C (PI-PLC), and its resulting minor lipid function in response to pollutant-induced stress, remains to be elucidated. In this work, we studied the effects of phenol treatment on the PLC pathway, using two lines of hairy root cultures (HRs) from Nicotiana tabacum as plant model systems: wild-type (WT) and double-transgenic (DT) HRs. We quantified several product formations such as PIP, PIP 2 , DGPP, and PA, which are mainly synthesized by specific lipid kinases belonging to the PLC pathway. In both HRs, phenol treatment significantly increased the formation of these compounds in a differential manner. In WT HRs, PA formation was twofold higher than in control. PIP 2 and PIP levels were about onefold higher than those of the controls while DGPP levels increased by 50%. In DT HRs, PIP levels were onefold higher than those of the controls while PIP 2 , DGPP, and PA levels increased by 120%. Phenol treatment also upregulated the PLC4 gene expression mainly in the first hours of exposure in both HRs, while the DGK1 gene expression was only upregulated in WT HRs after 24 h of treatment. These results show an active participation of the PLC pathway under phenol treatment suggesting that this signal pathway could be important in plant cell responses to phenol-induced stress.Fil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; ArgentinaFil: Racagni, Graciela Esther. Universidad Nacional de Río Cuarto; ArgentinaFil: Agostini, Elizabeth. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; ArgentinaFil: Medina, María I.. Universidad Nacional de Río Cuarto; Argentin

Plant growth promoting rhizobacteria improve the antioxidant status in Mentha piperita grown under drought stress leading to an enhancement of plant growth and total phenolic content

An experiment was conducted to investigate the effects of plant growth promoting rhizobacteria (PGPR) on Mentha piperita grown under drought stress. We performed root inoculation with strains of two PGPR species (Pseudomonas fluorescens WCS417 r and Bacillus amyloliquefaciens GB03) on peppermint plants and subjectedthem to moderate (MS) or severe drought stress (SS). To determine the growth-promoting potential and ability of PGPR to increase the drought tolerance in peppermint, different plant growth parameters were measured, along with the activities of antioxidant enzymes such as peroxidase (PX) and superoxide dismutase (SOD), as well as the non-enzymatic antioxidant proline and total phenolic content (TPC). In addition, to determine whether drought stress induces oxidative damage in peppermint, membrane lipid peroxidation was analyzed. An increment in the level of drought stress produced a reduction in plant growth, fresh weight, leaf number and leaf area. However, these negative effects of drought were mitigated in plants exposed to PGPR inoculation, resultingin significantly less reduction in the above growth traits related to plants not treated with PGPR, regardless of the severity of the drought treatment. In addition, drought-stressed plants treated with PGPR had a significantlyhigher total phenolic content than water-stressed plants without PGPR. Higher enzymatic activities were also observed in drought-stressed plants inoculated with PGPR. The proline content did not change in stressed plants, but inoculation reduced the amount of proline in the different stressed conditions tested. Membrane lipid peroxidation was also decreased in inoculated plants grown under drought conditions.These results are important as they illustrate the potential of PGPR to mitigate the adverse consequences of drought stress, and offer a way of increasing the tolerance of peppermint plants grown and TPC under water deficit conditions.Fil: Chiappero, Julieta. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; ArgentinaFil: Cappellari, Lorena del Rosario. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; ArgentinaFil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; ArgentinaFil: Palermo, Tamara Belen. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; ArgentinaFil: Banchio, Erika. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentin

A simple electrochemical immunosensor for sensitive detection of transgenic soybean protein CP4-EPSPS in seeds

Soybean is the most produced crop in Argentina, and 99 % corresponds to genetically modified soybean. One of the main produced varieties is Roundup Ready® soybean (RR), which was modified to express the enzyme CP4 5-enolpyruvylshikimate 3-phosphate synthase (CP4 EPSPS), which confers resistance to glyphosate, the main herbicide worldwide used. The possible impact of genetically modified organisms (GMO) has generated public concerns, thus increasing interest in the development of GMOs detection devices. In this work, an electrochemical immunosensor for CP4 EPSPS detection in soybean seeds was obtained, by using a gold electrode modified with an anti-CP4 EPSPS polyclonal antibody produced in our laboratory. The presented immunosensor resulted in a simple, low-cost, fast, and reproducible device. Also, labeling and/or signal amplification system was not necessary, since the sensor showed high sensibility with a low detection limit (lower at 0,038 % RR soybean, 38 ng mL−1 CP4 EPSPS).Fil: Farias, Marcos Ezequiel. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Correa, Nestor Mariano. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Niebylski, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Molina, Patricia Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentin

LC-ESI-MS/MS Method for the profiling of glycerophospholipids and its application to the analysis of tobacco hairy roots as early indicators of phenol pollution

The growing impact of organic and inorganic pollutants on the environment has led to the development of numerous strategies to counteract this effect. Among all, green technologies such as phytoremediation, has won relevance for its low cost as well as being an eco-friendly and sustainable process. In the past years, tobacco hairy roots (HRs) have become a good example of the effectiveness of plants as a tool for removing different pollutants. In this sense, the understanding of the intracellular mechanisms involved in the phytoremediation process has become of great interest. Glycerophospholipids (GLPs) are a key factor on intracellular signaling, given that they can activate different pathways, despite there is not enough information available about their levels and fluctuation under certain stress conditions. The aim of this work was to develop of a new chromatographic method coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) with selected reaction monitoring (SRM) and data dependent scan detection for the evaluation of GLP profile after the exposure to phenol, to get a better understanding of lipids turnover and their effect on signaling pathways. This highly sensitive method allows the identification, separation and quantification in less than a 20 min-run of the eleven most relevant GPL species present in hairy tobacco root extracts after phenol exposure, and is suitable for evaluating small changes in GPL levels.Fil: Flor, Sabrina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Dobrecky, Cecilia Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; ArgentinaFil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin