Protective Effects of Nano Zinc on Barki Ram Semen Viability

Sixty Barki semen ejaculates were collected using the artificial vagina. Semen samples were evaluated, pooled then subjected for processing using different concentrations of nano zinc (10, 50, 100 and 150µg/ml). Short-term chilled storage duration, absolute survivability index, post-thaw recovery, reduction rate, live percent, membrane integrity, acrosomal reaction, DNA integrity, head & tail’s diameter and DNA % were assessed. 150µg/ml of nano zinc can stabilize high motility % (80±0.54%) for about 11 days post dilution and chilling, then drastically dropped on day 18 (47±0.57%). Moreover, 150µg/ml recorded the best of absolute survivability index (33096±374), motility after dilution (86.67±1.05%), after cooling (87.50 ± 1.11%), after freezing- thawing process (56.67±1.66 %). Furthermore, 150µg/ml treated group keeping the highest records along the first three hours post-thawing (54.17 ± 1.53, 50.00 ± 1.29, and 47.50 ± 1.11%, respectively). Additionally, 150 µg/ml treated samples presented the highest viability index (180±3.47%), post-thaw recovery rate (65.47±2.26%), post-thawing live % (54.17±2.58%). While 150 µg/ml treated group showed the lowest reduction % (15.81 ± 3.12 %), acrosomal reaction (9.83±0.7%). Moreover, 150µg/ml treated samples recorded the largest head diameter and highest DNA % (52.87±1.74 and 97.51±0.30%, respectively), but showed the lowest tail diameter and tail DNA % (6.22±0.33 and 2.48±0.30%%, respectively). Furthermore, 150µg/ml treated group displayed the lowest comet %, tail moment and olive tail moment (8.950±0.02 %, 0.15±0.02 and 0.40±0.10, respectively) if compared with the other nano zinc treated groups. In conclusion: 150µg/ml of ZnO-NPs improves Barki ram semen processing, particularly, chilled semen viability, motility, longevity, membrane, and DNA integrity

Ameliorative Nexus of Nano Zinc on the Redox State of Chilled and Cryopreserved Barki Ram Semen

The current study aimed to modify rams’ semen redox hemostasis by different concentrations of Nano zinc. Using artificial vagina (15) semen ejaculates were collected, evaluated, and pooled then subjected to processing using different concentrations of nano zinc (10, 50, 100, and 150µg/ml). The concentration of alanine and aspartate transaminases enzyme (ALT-AST), alkaline phosphatase Enzyme (ALP), hydrogen peroxide (H2O2), malondialdehyde (MDA), superoxide dismutase (SOD), and total antioxidant capacity (TAC) of chilled and freeze-thawed Barki ram semen were assessed. Nano zinc significantly enhances semen quality in a dose-dependent manner. Where, 150µg/ml of nano zinc potentially reduced the extracellular concentrations of ALT (14.00 ± 1.01 and 28.58 ± 0.58 U/L), AST (39.67 ± 1.54 and54.83 ± 2.33 U/L), ALP (174.90 ± 1.90and 316.80 ± 1.90 U/L), and MDA (5.45 ± 0.02and 6.21 ± 0.01 nmol/ml) after both chilling and freeze-thawing process. Moreover, 150µg/ml treating dose induced an efficient production of SOD (7417±16.67 and 8600±76.38U/ml) after both chilling and freeze-thawing process, respectively. SOD is considered the most important antioxidant enzyme in the sperm cell that scavenges generated free radicles like H2O2. In this respect, 150µg/ml treating dose reported the lowest H2O2 level (0.042 ± 0.002 and0.065 ± 0.005 mM/L), and highest TAC concentration (1.62 ± 0.006and 1.69 ± 0.005 mM/L) after both chilling and freeze-thawing process, respectively. In conclusion, ZnO-NPs in semen extender can modify the sperm redox hemostasis in a dose-dependent manner. It reduces the extracellular transaminases and phosphatases leakage, augmenting SOD activity, and modifies TAC potential. ZnO-NPs supplementation to Barki sperm extender enhances its sperm quality and reduces cryo-injury

Ultrasonographic Monitoring of Uterine Involution in Postpartum Buffalo Cows

A description of normal ultrasonographic alternations of the uterus in the postpartum (PP) in buffaloes is essential for the diagnosis of pathological conditions and improves the ability to differentiate puerperal pathology from normal physiological changes. The current study aimed to characterize the changes in uterine features assessed ultrasonographically in normal parturient Egyptian buffaloes (n=20) during the post-calving period (Until Day 60 PP). In the current data, ultrasonographic findings indicated that the uterine involution was completed at 4-6 weeks PP in normal calved Egyptian buffaloes. Moreover, calving during the spring season and female new born calves were associated with an enhanced uterine involution. Early cyclicity positively influences endometrial thickness (during early PP), dorsal and ventral uterine curvature, and uterine lumen diameter (during late PP) as compared with the late cyclic group. In conclusion, the uterine involution is ended by the 6th weeks PP in normal parturient Egyptian buffaloes. Moreover, season and calves gender implicate in the changes associated with uterine involution in buffaloes. It is extremely recommended to employ ultrasound examinations in the dairy herd to improve the reproductive efficiency of females by reducing the days open and increasing the number of milking animals

Ultrasonographic Monitoring of Uterine Involution in Postpartum Buffalo Cows: Uterine involution in Buffaloes

A description of normal ultrasonographic alternations of the uterus in the postpartum (PP) in buffaloes is essential for the diagnosis of pathological conditions and improves the ability to differentiate puerperal pathology from normal physiological changes. The current study aimed to characterize the changes in uterine features assessed ultrasonographically in normal parturient Egyptian buffaloes (n=20) during the post-calving period (Until Day 60 PP). In the current data, ultrasonographic findings indicated that the uterine involution was completed at 4-6 weeks PP in normal calved Egyptian buffaloes. Moreover, calving during the spring season and female new born calves were associated with an enhanced uterine involution. Early cyclicity positively influences endometrial thickness (during early PP), dorsal and ventral uterine curvature, and uterine lumen diameter (during late PP) as compared with the late cyclic group. In conclusion, the uterine involution is ended by the 6th weeks PP in normal parturient Egyptian buffaloes. Moreover, season and calves gender implicate in the changes associated with uterine involution in buffaloes. It is extremely recommended to employ ultrasound examinations in the dairy herd to improve the reproductive efficiency of females by reducing the days open and increasing the number of milking animals