The changes in the amino and fatty acid profiles in the semifinished foodstuffs based on broiler meat and components of chicken eggs after different types of thermal treatment

The changes in the amino and fatty acid profiles in the semifinished foodstuffs (SFFs) based on broiler meat and coagulated chicken egg melange after different types of thermal treatment (water or steam boiling, braising, baking, frying) were studied. The amino acid profiles were determined on Knauer analyzer; tryptophan by standard method. The biological value of the treated products was assessed using amino acid balance coefficients calculated by the method of N. N. Lipatov. It was found that the changes in the initial amino acid profiles of the SFFs were the least after water and steam boiling; braising and baking were found to increase the contents of the essential amino acids. The amino acid profiles in the treated SFFs were close to the reference values. The best criteria of their biological value (coefficient of rationality of amino acid composition, comparable redundance) were found after water and steam boiling. It was found that all types of thermal treatments insignificantly affected the parameters of fatty acid balance within the SFFs; the changes found were primarily related to slight increase in total content of saturated fatty acids and increase in total content of polyunsaturated fatty acids (PUFAs) in compare to initial profiles, by 2.64–3.88% depending on the treatment type. The changes in ω‑6/ω‑3 PUFAs ratios were more substantial especially after braisin

Organization of the educational and production environment for the dual training of students of a professional educational organization

Рассматриваются возможности использования дуальной системы образования при подготовке квалифицированных специалистов в условиях социального партнерстваThe article deals with the possibilities of using the dual system of education in the training of qualified specialists in the conditions of social partnershi

Study of the association of gene polymorphism with the risk of non-communicable diseases in martial artists

Objective: to study the effect of genetic polymorphisms: rs rs9939609 (FTO gene), rs4994 (ADRB3 gene), rs1042713 (ADRB2 gene), rs2228570 (VDR gene), rs1801133 (MTHFR gene) on anthropometric and lipid metabolism indicators in athletes representing martial arts.Materials and methods: studies of anthropometric and biochemical parameters, genetic polymorphisms were carried out in 120 athletes (101 men and 19 women) who are engaged in martial arts. Anthropometric studies were performed by measuring height (cm), body weight (kg), followed by calculating body mass index (BMI, kg / m2). Biochemical nutritional status markers were determined using the ABX Pentra 400 analyzer (HORIBA ABX SAS, France) in an automatic mode. Genotyping was performed using allele­specific amplification using TaqMan probes complementary to polymorphic DNA regions and real­time detection of the results using reagent kits from Syntol, Russia. Studies were performed on the device CFX96 Real Time System (Bio­Rad, USA). Statistical processing of the results was performed using the PASW Statistics 20 system.Results: as a result of generic Diovan athletes martial artists on the risk of non­communicable diseases, discovered that the frequency of allele A of rs9939609 polymorphism of the FTO gene they have is 43.9 %, allele polymorphism rs4994 ADRB3 gene — 10.9 %, G allele of rs1042713 ADRB2 gene polymorphism — 52.6 %, G allele of the polymorphism rs2228570 VDR gene with 44.9 % and allele t of rs1801133 in the MTHFR gene to 36.7 %. An association was found between the value of anthropometric indicators in male martial artists and the presence of polymorphisms rs9939609 (FTO), rs1042713 (ADRB2) and rs2228570 (VDR).Conclusions: the reason for the identified dyslipidemia in martial artists may be not only the previously detected violations of the structure of their nutrition, but also the presence of certain genetic polymorphisms, in particular, rs4994 of the ADRB3 gene and rs1042713 of the ADRB2 gene

Study of Association of gene polymorphism with nutrition and nutritional status of martial arts athletes

Objective: studying the effect of genetic polymorphisms: rs1815739 (ACTN3 gene), rs2016520 (PPARD gene), rs1042713 (ADRB2 gene), rs1799945 (HFE gene) on anthropometric and lipid metabolism indicators in athletes representing martial arts. Materials and methods: studies of anthropometric and biochemical parameters, genetic polymorphisms were carried out in 83 athletes (64 men and 19 women) who were are engaged in martial arts. Anthropometric studies were performed by height (cm) and body weight (kg) measuring followed by calculating body mass index (BMI, kg / m2). Biochemical nutritional status markers were determined using the ABXPENTRA 400 analyzer (HORIBA ABX SAS, France) in an automatic mode. Genotyping was performed using allele-specific amplification using TaqMan probes complementary to polymorphic DNA regions and realtime detection of the results using reagent kits from Syntol, Russia. Studies were performed on the device «CFX96 Real Time System» («BIO-RAD», USA). Statistical processing of the results was performed using the PASW Statistics 20 system. Results: as a result of gene testing for endurance of combat athletes, it was found that the frequency of the T allele of the rs1815739 polymorphism (ACTN3 gene) was 47.5%, the PPARD gene ал20120120 allele G is 13.9%, the rs1042713 G allele of the ADRB2 gene was 51.9%, and the G allele of the rs1799945 polymorphism of the HFE gene was 19.3%. No association was found between the value of anthropometric parameters in athletes and the presence of the studied polymorphisms. Conclusions: the cause of identified dyslipidemia in combat athletes may be not only the disturbances in the structure of their nutrition that we found earlier, but also the presence of certain genetic polymorphisms

Insilico test of functional role of rs8068318 polymorphism of arterial hypertension-associated TBX2 candidate gene

In the course of this study, the functional role of rs8068318 polymorphism of the TBX2 candidate gene associated with the development of arterial hypertension was studied. The selection of the polymorphic locus was based on the data of the catalog of genome-wide association study (GWAS) of the National Human Genome Research Institute. The functional role was evaluated using online software: HaploReg (v4.1), GTExportal, and PolyPhen-

Improved Method for Preparation of 3-(1H-Indol-3-yl)benzofuran-2(3H)-ones

3-(1H-Indol-3-yl)benzofuran-2(3H)-ones were efficiently accessed via polyphosphoric acid-mediated condensation of 3-(2-nitrovinyl)-1H-indoles with phenols

Molecular-genetic factors of genital endometriosis

Article concerns data of the comparative analysis of polymorphic options of genes at patients with genital endometriosis and women in control grou

Genetic factors of pregnancy pathology

To study associations of genetic polymorphisms of cytokines with the risk of preeclampsia development. The study involved 250 pregnant women with preeclampsia and 245 women with a physiological pregnanc

tPCS as a method for correcting cardiac arrhythmias after percutaneous transluminal coronary angioplasty with stenting in patients with myocardial infarction

Objective: To study the effect of transcranial pulsed current stimulation (tPCS) therapy on cardiac arrhythmias developed after percutaneous transluminal coronary angioplasty (PTCA) with stenting in patients with myocardial infarction (MI).Material and methods: Characteristics of patient groups: the comparison group (n = 17) – with myocardial infarction, after PTCA with stenting, standard treatment; the main group (n = 21) – the same and tPCS therapy. Control points of the study: 1st day – Electrocardiography (ECG), Echocardiography (ECHO), Creatine phosphokinase (CPK), Creatine phosphokinase-MB (CPK-MB), Troponin-I, potassium, β-endorphin; 5th day – the same without ECHO; 10th day – the same and ECG with the determination of harmony and quantum of the electromagnetic flux of the cardiac cycle. PTCA was performed using drug-eluting stents. tPCS therapy was performed in pulsed bipolar mode, current strength 2 mA, current frequency 77.5 Hz, session duration 45 min.Results: In patients of the main group (against the background of tPCS therapy), the studied parameters of the cardiac cycle approached the optimal values. It was shown that intergroup differences in harmony (p = 0.002) and the size of the electromagnetic flux quantum of the cardiac cycle (p = 0.001) are statistically significant. Also, against the background of the tPCS therapy, the concentration of highly sensitive troponin-I is statistically significantly (p = 0.0042) lower by 109%. On the 5th and 10th days of the study, the serum concentration of β-endorphin in the main group was higher by 38.3 and 35.0% than in the comparison group (p < 0.05).Conclusion: The results of the study clearly demonstrate the cardioprotective and antiarrhythmic potential of tPCS therapy in patients with myocardial infarction and cardiac arrhythmias after PTCA with stenting