Proximate composition and mineral content in different, types of traditional TOGWA used in Tanzania as a weaning food

Tanzania Journal of Agricultural Sciences 2001, Vol. 4(2) :65-74Six types of traditional togwa (a traditional fermented gruel prepared from a mixture of malted and unmalted cereal grains), six simulated togwa and their ingredients were analysed for proximate composition, energy and mineral contents. The cereals used were maize (Zea mays), finger millet (Eleusine coracana) and sorghum (Sorghum bicolor). Malt was prepared from sorghum and finger millet. The: concentration of protein in traditional and simulated togwa was higher compared to their main ingredients. The ranges were 9.1-12.5, 9.9-12.9 and 8.7-12.0% respectively. Crude fibre was also higher in traditional and simulated togwa than in the main ingredients, while carbohydrate percent of DM and energy contents were lower than in the ingredients. Ash contents in traditional togwa was 1.44-2.21% and for simulated togwa 1.32-2.2%. Results for mineral contents indicated that phosphorus; magnesium and potassium were the major mineral constituents in both the traditional togwa and laboratory simulated samples. In the traditional and simulated togwa samples, phosphorus content ranged, respectively befween 196-334 and 191-332 (mg/100g), magnesium between 59.5-123.7 and 59.4-123 (mg/100g) and potassium between 167-315 and 167-313 (mg/100g). Calcium range was 3.95-12.32 (mg/100g) in traditional togwa and 3.40-11.92 (mg/100g) in simulated togwa compared to 5.57-14.59 (mg/100g) in the main ingredients. Iron, zinc and copper contents were slightly higher in traditional and in simulated togwa compared to their levels in the main ingredients. Iron ranged between 18.5-23.8 and 18.5-24.8 (mg/100g) in traditional and simulated samples respectively, and zinc ranged between 1.39- 1. 82 (mg/100g) in traditional togwa and 1.41-1.81 (mg/100g) in simulated samples. The contant of coppel in traditional and simulated togwa were 0. 33 - 0.69 and 0.32-0.70 (mg/100g), respectively. Apparently, the choice of cereal and malt and the processes of germination and fermentation for togwa production affects the proportions of nutrients found in the product. This results in different types of togwa with variation in proximate composition, energy density and mineral content

Proximate composition and mineral content in different, types of traditional TOGWA used in Tanzania as a weaning food

Tanzania Journal of Agricultural Sciences 2001, Vol. 4(2) :65-74Six types of traditional togwa (a traditional fermented gruel prepared from a mixture of malted and unmalted cereal grains), six simulated togwa and their ingredients were analysed for proximate composition, energy and mineral contents. The cereals used were maize (Zea mays), finger millet (Eleusine coracana) and sorghum (Sorghum bicolor). Malt was prepared from sorghum and finger millet. The: concentration of protein in traditional and simulated togwa was higher compared to their main ingredients. The ranges were 9.1-12.5, 9.9-12.9 and 8.7-12.0% respectively. Crude fibre was also higher in traditional and simulated togwa than in the main ingredients, while carbohydrate percent of DM and energy contents were lower than in the ingredients. Ash contents in traditional togwa was 1.44-2.21% and for simulated togwa 1.32-2.2%. Results for mineral contents indicated that phosphorus; magnesium and potassium were the major mineral constituents in both the traditional togwa and laboratory simulated samples. In the traditional and simulated togwa samples, phosphorus content ranged, respectively befween 196-334 and 191-332 (mg/100g), magnesium between 59.5-123.7 and 59.4-123 (mg/100g) and potassium between 167-315 and 167-313 (mg/100g). Calcium range was 3.95-12.32 (mg/100g) in traditional togwa and 3.40-11.92 (mg/100g) in simulated togwa compared to 5.57-14.59 (mg/100g) in the main ingredients. Iron, zinc and copper contents were slightly higher in traditional and in simulated togwa compared to their levels in the main ingredients. Iron ranged between 18.5-23.8 and 18.5-24.8 (mg/100g) in traditional and simulated samples respectively, and zinc ranged between 1.39- 1. 82 (mg/100g) in traditional togwa and 1.41-1.81 (mg/100g) in simulated samples. The contant of coppel in traditional and simulated togwa were 0. 33 - 0.69 and 0.32-0.70 (mg/100g), respectively. Apparently, the choice of cereal and malt and the processes of germination and fermentation for togwa production affects the proportions of nutrients found in the product. This results in different types of togwa with variation in proximate composition, energy density and mineral content

Characteristics of cheeses manufactured using pepsin from adult cattle Abomasa

Tanzania Journal of Agricultural Sciences 2001, Vol. 4(2) : 55-64The cheese making quality of pepsin extracted from adult cattle abomasa using dilute HCI (HP) and commercial vinegar (VP) was assessed using three types of cheeses namely Alpine, Tilister and Pastafilata (Pasta). The level of each extract was substituted with commercial calf rennet (CR) at 0, 25, 50, 75, 100%. Renneting time (RT) for cheese milk butterfat (%BF) and total nitrogen (%TN) losses in whey were assessed. Chemical composition and microbial quality of cheese samples obtained at different storage time intervals and their sensory quality at maturity were determined, RT and TN losses in Alpine cheses increased with increasing levels of pepsin from 41min and 0.49% in 25% pepsin to 58min, and 0.55% in 100% pepsin (HP) respectively, Chemical composition of all cheese types with different levels of HP and VP were significantly different (p 0.05). Coliform and yeast/mould counts in Tilster and Pasta cheeses were not significantly different while the Standard Plate Counts (SPC) in Tilster, and Pasta cheeses were significantly different. Sensory quality was not significantly different for smell and taste. However, bitterness in Pasta and appearance in Alpine and Pasta were significantly different (P 0.05) between levels of pepsin and types of extraction media for pepsin. HCI and vinegar extracted pepsin waa an apprqpriate renner substitute for the small scale cheese processors

Characteristics of cheeses manufactured using pepsin from adult cattle Abomasa

Tanzania Journal of Agricultural Sciences 2001, Vol. 4(2) : 55-64The cheese making quality of pepsin extracted from adult cattle abomasa using dilute HCI (HP) and commercial vinegar (VP) was assessed using three types of cheeses namely Alpine, Tilister and Pastafilata (Pasta). The level of each extract was substituted with commercial calf rennet (CR) at 0, 25, 50, 75, 100%. Renneting time (RT) for cheese milk butterfat (%BF) and total nitrogen (%TN) losses in whey were assessed. Chemical composition and microbial quality of cheese samples obtained at different storage time intervals and their sensory quality at maturity were determined, RT and TN losses in Alpine cheses increased with increasing levels of pepsin from 41min and 0.49% in 25% pepsin to 58min, and 0.55% in 100% pepsin (HP) respectively, Chemical composition of all cheese types with different levels of HP and VP were significantly different (p 0.05). Coliform and yeast/mould counts in Tilster and Pasta cheeses were not significantly different while the Standard Plate Counts (SPC) in Tilster, and Pasta cheeses were significantly different. Sensory quality was not significantly different for smell and taste. However, bitterness in Pasta and appearance in Alpine and Pasta were significantly different (P 0.05) between levels of pepsin and types of extraction media for pepsin. HCI and vinegar extracted pepsin waa an apprqpriate renner substitute for the small scale cheese processors

Optimisation of activity and storage stability of crude pepsin extracted from adult cattle Abomasa for cheese making

Tanzania Journal of Agricultural Sciences 2001, Vol. 4(1) : 29-36The study conducted to investigate the activity and storage stability of crude pepsin extract for use as rennet substitute in cheese processing. The conditions tested were regions of the abomasa, fresh versus dry abomasa drying methods, maceration temperatures (37oC, 24-28oC or 15oC); maceration pH 1-6 for 6 days; activation pH (pH 1-4) and clarification by centrifugation or use of chemicals. The shelf life of the extract was evaluated under storage temperatures ranging from deep freezing at -20oC refrigeration temperatures (<10 oC) and room temperature. Results showed that fundic region of the abomasa had 70.8% pepsin while the pyloric region had only 27% pepsin. Milk clotting activity (MCA) was 1978 and 1800 pepsin units per ml (PU/ml) for sun and shade-dried abomasa respectively. Concentrations of 5, 10, 15, 20, 25% NaCl gave 1845, 2189, 1596, 1021, 462 MCA respectively. Maceration at pH 3 for 3 days at 37 oC gave the highest enzymatic activity (5536 PU/ml). Clearing of the extract by centrifugation at 3000g/15 min gave an extract with 902.3 PU/ml. Clarification by use of di-sodium phosphate (Na2HSO4) gave extract of 1679.1 PU/ml. The enzyme activity of the extract stored under deep freezer temperature was sibnificantly higher (p < 0.05) than ambient and refrigeration temperatures. However, they were significantly lower (p< 0. 001) than the activity of the extract before storage. Therefore, the best pepsin extraction conditions were found to be the use of fundic region of sundried abomasa, macerated at 37oC in an extraction solution containing 5-10% salt at pH 3 for 3 days and clarified after activation at pH2 for 20 min followed by centrifugation of sediment at 3000g/15 min or by use of anhydrous disodium phosphate. The clarified extract could retain its activity for 16 weeks when stored at - 20oC in single use portion

Optimisation of activity and storage stability of crude pepsin extracted from adult cattle Abomasa for cheese making

Tanzania Journal of Agricultural Sciences 2001, Vol. 4(1) : 29-36The study conducted to investigate the activity and storage stability of crude pepsin extract for use as rennet substitute in cheese processing. The conditions tested were regions of the abomasa, fresh versus dry abomasa drying methods, maceration temperatures (37oC, 24-28oC or 15oC); maceration pH 1-6 for 6 days; activation pH (pH 1-4) and clarification by centrifugation or use of chemicals. The shelf life of the extract was evaluated under storage temperatures ranging from deep freezing at -20oC refrigeration temperatures (<10 oC) and room temperature. Results showed that fundic region of the abomasa had 70.8% pepsin while the pyloric region had only 27% pepsin. Milk clotting activity (MCA) was 1978 and 1800 pepsin units per ml (PU/ml) for sun and shade-dried abomasa respectively. Concentrations of 5, 10, 15, 20, 25% NaCl gave 1845, 2189, 1596, 1021, 462 MCA respectively. Maceration at pH 3 for 3 days at 37 oC gave the highest enzymatic activity (5536 PU/ml). Clearing of the extract by centrifugation at 3000g/15 min gave an extract with 902.3 PU/ml. Clarification by use of di-sodium phosphate (Na2HSO4) gave extract of 1679.1 PU/ml. The enzyme activity of the extract stored under deep freezer temperature was sibnificantly higher (p < 0.05) than ambient and refrigeration temperatures. However, they were significantly lower (p< 0. 001) than the activity of the extract before storage. Therefore, the best pepsin extraction conditions were found to be the use of fundic region of sundried abomasa, macerated at 37oC in an extraction solution containing 5-10% salt at pH 3 for 3 days and clarified after activation at pH2 for 20 min followed by centrifugation of sediment at 3000g/15 min or by use of anhydrous disodium phosphate. The clarified extract could retain its activity for 16 weeks when stored at - 20oC in single use portion