Integrating data mining within a strategic knowledge management framework: A platform for sustainable competitive advantage within the Australian Minerals and Metals Mining sector

<p>N = 4–10 mice.</p

Calcein accumulation in guinea pig primary brain endothelial cells (n = 6) presented as % of control with increasing doses of sertraline (SERT) and 10⁻⁴ of verapamil (VPL).

<p>Calcein accumulation was assessed at (A) 15 min, (B) 60 min, (C), 120 min and (D) 240 min. * indicates p<0.05 and ** indicates p<0.01 compared to control cells.</p

Accumulation of [3H] digoxin in FVB mice (n = 4–9) co-administered sertraline (10 mg/kg; closed bars) or saline (control; open bars).

<p>Mice were euthanized at various time points (1 m, 5 min, 15 min, 1 h, 4 h, 12 h, 24 h) and DPM measured in the testes, heart and plasma. All data is expressed as mean±SEM. (A) testes∶plasma DPM ratio, (B) heart∶plasma DPM ratio. * indicates p<0.05 vs. control. ** indicates p<0.01 vs. control.</p

P-gp function in GD50 BECs is unresponsive to pro-inflammatory cytokines.

<p>P-glycoprotein (P-gp) activity in brain endothelial cell (BEC) cultures derived from gestational day (GD) 50 male guinea pigs following treatment with 10⁰–10⁴ pg/mL A–C) interleukin-1β (IL-1β), D–F) interleukin-6 (IL-6) or G–I) tumor necrosis factor-α (TNF-α) for 1, 4 or 24 hours (N = 4). P-gp activity is displayed as percent change from untreated control cells (zero line). Values displayed as mean ± S.E.M.</p

Pro-inflammatory cytokines inhibit P-gp function in GD65 BECs in a dose-dependent manner.

<p>P-glycoprotein (P-gp) activity in brain endothelial cell (BEC) cultures derived from gestational day (GD) 65 male guinea pigs following treatment with 10⁰–10⁴ pg/mL A–C) interleukin-1β (IL-1β), D–F) interleukin-6 (IL-6) or G–I) tumor necrosis factor-α (TNF-α) for 1, 4 or 24 hours (N = 4). P-gp activity is displayed as percent change from untreated control cells (zero line). Values displayed as mean ± S.E.M. A significant difference from control indicated by (*) P<0.05; (**) P<0.01.</p

The inhibitory effects of pro-inflammatory cytokines are specific to P-gp and do not alter non-specific esterase activity.

<p>Postnatal day 14 male guinea pig BEC A) P-gp activity (using Rhodamine 123 as a P-gp substrate) following 24 hour treatment with 3.3×10³ pg/mL interleukin-1β (IL-1β), interleukin-6 (IL-6) or tumor necrosis factor-α (TNF-α) (N = 6); and B) non-specific esterase activity following 24 hour treatment with 3.3×10³ pg/mL IL-1β, IL-6 or TNF-α (N = 6). P-gp activity is displayed as percent change from untreated control cells (zero line). Values displayed as mean ± S.E.M. A significant difference from control indicated by (**) <i>P</i><0.01.</p

The inhibitory effects of pro-inflammatory cytokines on P-gp function are greatest in PND14 BECs.

<p>P-glycoprotein (P-gp) activity in brain endothelial cell (BEC) cultures derived from postnatal day (PND) 14 male guinea pigs following treatment with 10⁰–10⁴ pg/mL A–C) interleukin-1β (IL-1β), D–F) interleukin-6 (IL-6) or G–I) tumor necrosis factor-α (TNF-α) for 1, 4 or 24 hours (N = 8). P-gp activity is displayed as percent change from untreated control cells (zero line). Values displayed as mean ± S.E.M. A significant difference from control indicated by (*) P<0.05; (**) P<0.01.</p

Pro-inflammatory cytokines inhibit <i>abcb1</i> mRNA expression.

<p><i>Abcb1</i> mRNA expression in brain endothelial cell (BEC) cultures derived from postnatal day (PND) 14 male guinea pigs following treatment with 10³ and 3.3×10³ pg/mL A–B) interleukin-1β (IL-1β), C–D) interleukin-6 (IL-6) or E–F) tumor necrosis factor-α (TNF-α) for 4 (A,C,E) or 24 hours (B,D,F) (N = 7–8). Expression is displayed as percent of untreated control cell expression (i.e. 100% line) and taken over the reference gene, <i>beta-actin</i>. Values displayed as mean ± S.E.M. A significant difference from control indicated by (**) P<0.01; (***) P<0.001.</p

Baseline P-gp Activity in BECs increases with developmental age.

<p>P-glycoprotein (P-gp) activity in brain endothelial cell (BEC) cultures derived from gestational day (GD) 40, 50, 65 and postnatal day (PND) 14 male guinea pigs (N = 4). P-gp activity is displayed as percent change from GD40 BECs (zero line). P-gp function was calculated over relative cell count. Values displayed as mean ± S.E.M. A significant difference from GD40 indicated by (*) P<0.05; (***) P<0.001.</p

Rate of fetal death/reabsorption after acute LPS exposure.

<p>Rate of fetal death/reabsorption after acute LPS exposure.</p