Venn diagram of bacterial OTUs (at 97% identity) common to the pyrosequencing libraries for eggs (E), L2 larvae (L2) and workers (W) of <i>N</i>. <i>arborum</i>.

<p>Numbers in bold indicate the number of OTUs and numbers in italics the relative abundance of the corresponding OTUs in each stage where they are detected. Details of the common OTUs are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140014#pone.0140014.s003" target="_blank">S2 Table</a>.</p

Sampling depth and number of taxa at OTU level (at 97% identity), at genus level and phylum level by 16S rRNA gene pyrosequencing from the various life stages of <i>N</i>. <i>arborum</i>. For eggs, L2 larvae and workers, the numbers of taxa are based on a sub-sample of 1367 reads.

<p>*For L1 larvae, they are based on 50 sequences excluding <i>Wolbachia</i>. They are given for illustration only and are not included in the total for the taxonomic composition, which refers to unique taxa.</p><p>** For the taxonomic composition, the total number of taxa at the phylum, genus and OTU levels is the sum of the individual taxa from the three pyrotag libraries, taking account of the taxa common to different stages.</p><p>Sampling depth and number of taxa at OTU level (at 97% identity), at genus level and phylum level by 16S rRNA gene pyrosequencing from the various life stages of <i>N</i>. <i>arborum</i>. For eggs, L2 larvae and workers, the numbers of taxa are based on a sub-sample of 1367 reads.</p

Phylum-level distribution of bacterial taxa from pyrotag libraries based on the 16S rRNA gene from eggs and the guts of the first instar (L1 larvae), second instar (L2 larvae) and workers of <i>N</i>. <i>arborum</i>.

<p>For each stage, the inner pie chart corresponds to the relative abundance of reads affiliated to each phylum. The outer pie chart corresponds to the number of OTUs for each phylum as a fraction of the total number of OTUs. *The charts for L1 larvae are based on 50 sequences excluding <i>Wolbachia</i> unlike the charts for eggs, L2 larvae and workers stages which are based on subsamples of 1367 reads. “Minor phyla” correspond to the pool of all phyla with <1% of reads and ≤ 5 OTUs in well sequenced libraries (eggs, L2 larvae, workers).</p

Relative abundances of the genus-level bacterial groups in eggs (E), the first instar larvae (L1), the second instar larvae (L2) and the worker caste (W) of <i>N</i>. <i>arborum</i>.

<p>Only genera > 0.5% in at least one stage are presented. The remaining genera are included in “Others”. The detailed classification with all the taxonomic levels is provided in the interactive spreadsheet (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140014#pone.0140014.s002" target="_blank">S1 Table</a>). * The relative abundances for the L1 larval stage are based on 50 sequences excluding <i>Wolbachia</i>.</p

Phylogenetic tree illustrating the position of Spirochaetes-related OTUs from 16S rRNA gene libraries.

<p>The tree was constructed using the Maximum Likelihood-method implemented in MEGA version 6.0 and the Tamura-Nei model (analysis, 1000 bootstraps). Only bootstrap values >50% are presented. Clones from this study are referred to as GL and GLS (from the second instar larvae) and GO (from workers) and numbers in brackets correspond to NCBI accession numbers of sequences. * indicates sequences undergoing NCBI submission. Branches in green are entirely made of sequences from termites.</p