z-stack of the postcentral gyrus of the mouse brain in vivo.

<p>(A)–(E) show blood vessels (arrows), stained by Texas Red 70 kDa Dextran in 5, 60, 120, 200 and 300 µm underneath the brain surface. Internal detectors were used with maintained settings for gain and offset. a, left column: Without laser adjustment all blood vessels within the 300 µm range were visible, but appeared darker and blurry below a depth of 200 µm. b, right column: the same region acquired with adjustment of the laser power. In contrast to the liver and kidney a much deeper penetration into the tissue was possible.</p

MPM imaging conditions in a kidney of a MWF rat in vivo.

<p>(A) Image of a superficial glomerulus (g) and a distal tubule (dt) in the kidney of a MWF rat. Proximal tubules (pt) showed green autofluorescence. Intra- and extraglomerular capillaries (arrows) were visualized with Texas Red-labeled 70 kDa dextran. The glomerulus was located directly underneath the renal capsule allowing high resolution imaging. (B) Histology of a MWF rat kidney section with many superficial glomeruli (arrows).</p

Depth of glomeruli in 10-week-old adult mice.

<p>Box-plot shows mean±25% (boxes) and the 5–95% percentile (whiskers). (A) Box-plot of all ten strains arranged according to the 25% percentile. (B) Box-plot of the peer group (129, CB6F1, C3H/N, NMRI, SJL, DBA/2, FVB, CD-1) vs. Bl/6 and BALB/c.</p

Depth of glomeruli in 4-week-old juvenile mice.

<p>Box-plot shows mean±25% (boxes) and the 5–95% percentile (whiskers). (A) Box-plot of all ten strains arranged according to the 25% percentile. (B) Box-plot of the peer group (129, CB6F1, NMRI, SJL, FVB, CD-1) vs. Bl/6, BALB/c, C3H/N and DBA/2.</p