Additional file 2: of Evaluation of the performances of six commercial kits designed for dengue NS1 and anti-dengue IgM, IgG and IgA detection in urine and saliva clinical specimens

Concordance between RDTs and IPC ELISAs for NS1, anti-DENV IgG/IgM and anti-DENV IgA detection in saliva and urine. (PDF 29 kb

Additional file 1: of Evaluation of the performances of six commercial kits designed for dengue NS1 and anti-dengue IgM, IgG and IgA detection in urine and saliva clinical specimens

Characteristics of the rapid diagnostic tests for NS1, anti-DENV IgG/IgM and anti-DENV IgA detection in saliva and urine according to the manufacturer’s instructions. (PDF 87 kb

Partial dependence plots for the most influential variables explaining NS1 antigen detection in plasma, urine and saliva.

<p>Partial dependence plots for the most influential variables explaining NS1 antigen detection in plasma, urine and saliva.</p

Partial dependence plots for the most influential variables explaining anti-DENV antibodies detection in plasma, urine and saliva.

<p>Partial dependence plots for the most influential variables explaining anti-DENV antibodies detection in plasma, urine and saliva.</p

Mean viral load measured by qRT-PCR in plasma, urine and saliva.

<p>Mean viral load measured by qRT-PCR in plasma, urine and saliva.</p

Detection of anti-DENV antibodies in plasma, urine and saliva.

<p>Percentage of samples that tested positive by MAC-ELISA (A), AAC-ELISA (B) and IgG indirect ELISA (C) in urine, saliva and plasma samples according to time after onset of fever (D for day, W for week and M for month).</p

Partial dependence plots for the most influential variables explaining viral genome detection in plasma, urine and saliva.

<p>Partial dependence plots for the most influential variables explaining viral genome detection in plasma, urine and saliva.</p

Results of viral genome and NS1 protein detection in saliva, urine and plasma samples collected from the same patients at the same time-points by time of sampling after onset of fever.

<p>NA: No sample Available</p><p>Percentage of positivity (number of positive samples/total number of samples tested).</p

Results of the Boosted Regression Trees analysis for the detection of anti-DENV antibodies in plasma, urine and saliva.

<p>daof: number of days between the onset of the fever and the day of sampling</p><p>status: immune status of the patient determined by HIA (primary versus secondary infection)</p><p>classif: severity of the disease according to the 1997 and the 2009 WHO guidelines</p><p>antibodies: level of the corresponding immunoglobulin isotype in the plasma estimated by the measure by ELISA of the optical density</p><p>*Estimate of the model performance</p><p>Results of the Boosted Regression Trees analysis for the detection of anti-DENV antibodies in plasma, urine and saliva.</p

Positivity rates of NS1 protein detection in plasma, urine and saliva.

<p>Percentage of samples that tested positive for NS1 protein by capture ELISA in urine, saliva and plasma, by day of sampling after onset of fever (n = 193, 197 and 217 patients with a confirmed dengue infection included for NS1 detection in urine, saliva and plasma, respectively).</p