4 research outputs found
Peptide Nanofiber Substrates for Long-Term Culturing of Primary Neurons
The
culturing of primary neurons represents a central pillar of neuroscience
research. Primary neurons are derived directly from brain tissue and
recapitulate key aspects of neuronal development in an in vitro setting.
Unlike neural stem cells, primary neurons do not divide; thus, initial
attachment of cells to a suitable substrate is critical. Commonly
used polylysine substrates can suffer from batch variability owing
to their polymeric nature. Herein, we report the use of chemically
well-defined, self-assembling tetrapeptides as substrates for primary
neuronal culture. These water-soluble peptides assemble into fibers
which facilitate adhesion and development of primary neurons, their
long-term survival (>40 days), synaptic maturation, and electrical
activity. Furthermore, these substrates are permissive toward neuronal
transfection and transduction which, coupled with their uniformity
and reproducible nature, make them suitable for a wide variety of
applications in neuroscience
First Demonstration of Positive Allosteric-like Modulation at the Human Wild Type Translocator Protein (TSPO)
We
show that changing the number and position of nitrogen atoms
in the heteroatomic core of a pyrazolopyrimidine acetamide is sufficient
to induce complex binding to wild type human TSPO. Only compounds
with this complex binding profile lacked intrinsic effect on glioblastoma
proliferation but positively modulated the antiproliferative effects
of a synthetic TSPO ligand. To the best of our knowledge this is the
first demonstration of allosteric-like interaction at the wild type
human TSPO
Peptide Nanofiber Substrates for Long-Term Culturing of Primary Neurons
The
culturing of primary neurons represents a central pillar of neuroscience
research. Primary neurons are derived directly from brain tissue and
recapitulate key aspects of neuronal development in an in vitro setting.
Unlike neural stem cells, primary neurons do not divide; thus, initial
attachment of cells to a suitable substrate is critical. Commonly
used polylysine substrates can suffer from batch variability owing
to their polymeric nature. Herein, we report the use of chemically
well-defined, self-assembling tetrapeptides as substrates for primary
neuronal culture. These water-soluble peptides assemble into fibers
which facilitate adhesion and development of primary neurons, their
long-term survival (>40 days), synaptic maturation, and electrical
activity. Furthermore, these substrates are permissive toward neuronal
transfection and transduction which, coupled with their uniformity
and reproducible nature, make them suitable for a wide variety of
applications in neuroscience
Peptide Nanofiber Substrates for Long-Term Culturing of Primary Neurons
The
culturing of primary neurons represents a central pillar of neuroscience
research. Primary neurons are derived directly from brain tissue and
recapitulate key aspects of neuronal development in an in vitro setting.
Unlike neural stem cells, primary neurons do not divide; thus, initial
attachment of cells to a suitable substrate is critical. Commonly
used polylysine substrates can suffer from batch variability owing
to their polymeric nature. Herein, we report the use of chemically
well-defined, self-assembling tetrapeptides as substrates for primary
neuronal culture. These water-soluble peptides assemble into fibers
which facilitate adhesion and development of primary neurons, their
long-term survival (>40 days), synaptic maturation, and electrical
activity. Furthermore, these substrates are permissive toward neuronal
transfection and transduction which, coupled with their uniformity
and reproducible nature, make them suitable for a wide variety of
applications in neuroscience