Additional file 1 of Photosensitizing deep-seated cancer cells with photoprotein-conjugated upconversion nanoparticles

Supplementary Material 1: Additional file 1: Figure S1. Elemental analysis of CS-UCNPs by energy-dispersive X-ray spectroscopy. Figure S2. X-ray diffraction patterns of oleic acid-capped Co-UCNPs and CS-UCNPs. Figure S3. TEM image of Co-UCNPs. Figure S4. TEM images of Co-UCNPs and CS-UCNPs. Figure S5. TEM images of CS-UCNPs. Figure S6. Effect of Yb3+ concentratons on the ET efficiency in UCNPs. Figure S7. SDS-PAGE and fluorescent gel images of KR and KR-LP. Figure S8. FT-IR spectrum of KR-LP. Figure S9.In vitro stability of CS-UCNP-KR-LP over a two-week period using PL intensity and polydispersity index. Figure S10. Effect of Er3+ concentrations (2−10%) in CS-UCNP-NH2 on the PL decay time. Figure S11. Flow cytometric analysis of intracellular ROS generation using DCFDA with CS-UCNPs or Co-UCNPs. Figure S12. MTT assay of cell viability according to nanocomposite concentration for 5 cancer cell lines. Figure S13. MTT assay of cell viability according to irradiation time for 5 cancer cell lines. Figure S14. MTT assay of cell viability of MCF-7 cells without either NIR irradiation or nanocomposites. Figure S15. Measurement of cellular uptake of three different CS-UCNPs using ICP-MS in cancer cells. Figure S16. Experimental setup for evaluating the tissue-penetrating effect of NIR irradiation on CS-UCNP-KR-LP in MCF-7 cells