73 research outputs found

    Photoinduced luminescence quenching and insolubilization of star-like branched organosilicon compounds

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    This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in Tooru Sonoda, Hidayat Rahmat, Masanori Ozaki, Katsumi Yoshino, Wolfgang Schneider, Kyung Koo Lee, Akinobu Naka, and Mitsuo Ishikawa, Appl. Phys. Lett. 75, 2193 (1999) and may be found at https://doi.org/10.1063/1.124961

    CLINICAL AND EXPERIMENTAL STUDIES ON THE ERYTHROCYTES WHICH INCLUDE JOLLY'S BODIES IN HORSES

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    The erythrocytes including JOLLY's bodies in horses were investigated clinically and experimentally. The conclusions arrived at may be summarized as follows. 1. Erythrocytes which include JOLLY's bodies were always present in the blood of horses. The mean number of appearance per one hundred thousand erythrocytes was 10.1 in the clinically healthy horses and 18.7 in the horses affected with infectious anemia, respectively. 2. In continual observations of a colt being rendered anemic by blood-letting, a slight tendency of increase in the number of appearances of erythrocytes with JOLLY's bodies was observed during the period from the maximum anemia to near the time of recovery from anemia. 3. Difference in the number of appearances of erythrocytes with JOLLY's bodies between peripheral blood and bone marrow fluid was not remarkable in the clinically healthy horses; however in the infectious anemia horses, a slight increase in number of JOLLY's bodies found was noted in the bone marrow fluid. 4. There was no special relation between the number of appearances of erythroblasts and the number of appearances of erythrocytes which include JOLLY's bodies. 5. There were no differences in size between the erythrocytes with JOLLY's bodies and the erythrocytes with no JOLLY's body from the normal horses or from the horses affected with infectious anemia. 6. Erythrocytes with both JOLLY's bodies and so-called supravital stainable substances were not found at all in the peripheral blood of clinically healthy horses and horses affected with infectious anemia

    LYMPHOCYTES OF CANINE PERIPHERAL BLOOD IN ELECTRON MICROSCOPY

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    The fine structure of the lymphocytes in the peripheral blood of clinically normal dogs was observed by the use of an electron microscope. The results thus obtained were summarized as follows. 1) The fine structure of the canine lymphocytes was fundamentally similar to that of the human lymphocytes. 2) The maculous appearance of the nucleoplasm was less marked than that of the canine neutrophils. 3) A nuclear body was observed in the nuclei of some lymphocytes. 4) An area consisting of fine dust-like particles and without any microorganelles was observed in the cytoplasm of some lymphocytes. 5) The size of the mitochondria of the lymphocytes was larger than that of the canine monocytes. 6) Some of the dense granules in the cytoplasm seemed to have resulted from the accumulation of a dense mass in the mitochondria

    HEMATOLOGICAL OBSERVATIONS OF LEUKOCYTES COLLECTED BY WATER METHOD FROM BOVINE PERIPHERAL BLOOD

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    An attempt was made to collect a large number of leukocytes without red cells from bovine peripheral blood by the use of the water method improved by the authors, then they were examined hematologically. 1) In the collection of leukocytes by the water method, it was found that using blood and water, in the ratio of 1 to 1.5,with the restoration of isotonicity after 20 seconds was the most favorable condition for bovine blood. 2) In the light microscopy of the collected leukocytes smears stained with Giemsa stain, a significant decrease of normal cells and an increase of destructed cells was observed in comparison with those of the untreated control blood, respectively, but in so-called ghost cells, no significant changes were observed. 3) A significant increase of so-called dead cells was observed in the collected cells in comparison with those of the untreated control blood. 4) By the treatment with water, the phagocytic activities of carbon particles were decreased significantly in monocytes and eosinophils, but in neutrophils no significant changes were observed in comparison with those of the untreated control blood. 5) In the electron microscopy of the collected leukocytes, the defects of the fine structure of the cells were not so marked that almost all the collected cells distinguished their kind of cells by the characteristics of their fine structures. 6) In the differential count of the collected leukocytes, a significant increase of neutrophils and a decrease of lymphocytes was observed, but no significant changes were observed in monocytes, eosinophils and basophils in comparison with the untreated control blood

    EOSINOPHILS OF OVINE PERIPHERAL BLOOD IN ELECTRON MICROSCOPY

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    The fine structures of the eosinophils of the peripheral blood obtained from 5 clinically normal sheep were examined under an electron microscope. The results thus obtained were summarized as follows : 1) The cells with nuclei of two to four lobes were most commonly observed on the cut planes. 2) The nuclear lobes of the cells showed a clear maculous appearance with two distinct densities, one light and the other dark, according to the amount of the chromatin condensation. 3) On the basis of the internal structures of the specific granules, they were classified into six types and the basic type of the granules seemed to be those containing middle plates. 4) A small number of mitochondria measuring 0.3 by 0.7ΞΌ on the average, Golgi complex, a number of smooth- and a few rough-surfaced endoplasmic reticulum, and some other micro-organelles were observed in the cytoplasm

    ELECTRON MICROSCOPIC STUDIES ON THE MORPHOLOGY OF MICROFILARIAE OF DIROFILARIA IMMITIS : I OBSERVATIONS OF THE WHOLE BODY

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    Using the electron microscope, the structure of the body surface of microfilariae of Dirofilaria immitis obtained from dogs with heart filariasis was observed. The width of the striations on the body surface and the shape of the tail were described

    FELINE NEUTROPHILS IN ELECTRON MICROSCOPY

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    The neutrophils of the cardiac blood obtained from 6 clinically healthy adult cats were observed by the use of electron microscopy. The fine structures of them were basically similar to those of the human and other animals. However, the following interesting findings were obtained. 1) The specific granules of the cells were classified into 3 types such as the homogeneously compact ones with high electron density, less dense ones with granular matrix and light or almost empty ones. 2) As the internal structure of the specific granules, so-called middle plates were observed in some of the granules with high electron density
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