4 research outputs found
Comprehensive Profiling of N‑Linked Glycosylation Sites in HeLa Cells Using Hydrazide Enrichment
The adenocarcinoma cell line HeLa serves as a model
system for cancer research in general and cervical cancer in particular. In
this study, hydrazide enrichment in combination with state-of-the art
nanoLC−MS/MS analysis was used to profile N-linked glycosites in HeLa
cells. N-Linked glycoproteins were selectively enriched in HeLa cells by
the hydrazide capture method, which isolates all glycoproteins
independent of their glycans. Nonglycosylated proteins were removed
by extensive washing. N-Linked glycoproteins were identified with the
specific NXT/S motif and deamidated asparagine (N). Deglycosylation
was carried out in both H_2 (^16)O and H_2 ^(18)O to confirm the deamidation.
NanoLC−MS/MS analysis indicated that the method selectively enriched
at least 100 fold N-linked glycosites in HeLa cells. When both the
membrane and cytosolic fractions were used, a total of 268 unique N-glycosylation
sites were identified corresponding to 106 glycoproteins.
Bioinformatic analysis revealed that most of the glycoproteins identified
are known to have an impact on cancer and have been proposed as
biomarkers
Eating behaviour and eating disorders in students of nutrition sciences
Objective
Sometimes the suspicion is put forward that nutrition students show more disordered eating patterns, which may be among the motivating factors to study nutrition. At the same time, it is not clear whether the students’ increasing knowledge about diet and nutrition is associated with a more healthy eating behaviour or with an unhealthy obsession with food choices.
Design
Cross-sectional comparison of nutrition students from German universities during the first year of their studies (n 123) and during higher semesters (n 96), with a control group from other study programmes (n 68 and n 46, respectively). Dietary restraint, disinhibition, the tendency towards orthorexia nervosa and healthy food choices were assessed using a questionnaire.
Results
Nutrition students showed higher levels of dietary restraint than the control group. Disinhibition and orthorexia nervosa did not differ between nutrition students and controls. Orthorexic tendencies were lower in the more advanced nutrition students. Healthy food choices did not differ among students in the first year. More advanced nutrition students showed healthier food choices, whereas the corresponding controls showed slightly more unhealthy food choices.
Conclusions
Nutrition students, more than other students, tend to restrict their food intake in order to control their weight, but they do not have more disturbed or disordered eating patterns than other students. Moreover, during the course of their studies, they adopt slightly more healthy food choices and decrease their tendency to be obsessive in their eating behaviour