1 research outputs found
Altering the Communication Networks of Multispecies Microbial Systems Using a Diverse Toolbox of AI-2 Analogues
There have been intensive efforts to find small molecule
antagonists for bacterial quorum sensing (QS) mediated by the “universal”
QS autoinducer, AI-2. Previous work has shown that linear and branched
acyl analogues of AI-2 can selectively modulate AI-2 signaling in
bacteria. Additionally, LsrK-dependent phosphorylated analogues have
been implicated as the active inhibitory form against AI-2 signaling.
We used these observations to synthesize an expanded and diverse array
of AI-2 analogues, which included aromatic as well as cyclic C-1-alkyl
analogues. Species-specific analogues that disrupted AI-2 signaling
in <i>Escherichia coli</i> and <i>Salmonella typhimurium</i> were identified. Similarly, analogues that disrupted QS behaviors
in <i>Pseudomonas aeruginosa</i> were found. Moreover, we
observed a strong correlation between LsrK-dependent phosphorylation
of these acyl analogues and their ability to suppress QS. Significantly,
we demonstrate that these analogues can selectively antagonize QS
in single bacterial strains in a physiologically relevant polymicrobial
culture