Live birth rate of offspring upon transgenesis by PNI or EEZy.

<p>Live birth rate of offspring upon transgenesis by PNI or EEZy.</p

Embryo toxicity of EEZy as compared to pronuclear injection.

<p>(A) Assessment of embryo development as percentage of developed blastocysts from zygotes after EEZy. Untreated zygotes are compared to zygotes electroporated with Opti-MEM (Mock) or CRISPR/Cas9 components targeting the <i>Gt(ROSA)26Sor</i> locus (CRISPR). Data represent three independent experiments. (B) Quantification of viable <i>Nphs2</i>-targeted zygotes from four experiments immediately after PNI compared to electroporated zygotes and (C) the correspondingly developed blastocysts. (D) Developed blastocyst calculated from the number of zygotes before transgenesis in (B). (E) Quantification of RFLP genotyping from four independent experiments for HDR efficiency in blastocysts from zygotes after PNI compared to electroporated zygotes from the experiments depicted in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0196891#pone.0196891.g001" target="_blank">Fig 1B and 1D</a>, right columns. Data are means ± standard deviation. *p < 0.05, **p < 0.01, ns = non-significant. N = total number of embryos analyzed.</p