DAVID GO term analysis of genes enriched 5-fold in Lonza and ScienCell fetal astrocytes.

<p>DAVID GO term analysis of genes enriched 5-fold in Lonza and ScienCell fetal astrocytes.</p

Summary pathways analyzed in microarray.

<p>Summary pathways analyzed in microarray.</p

Transcription factors upregulated in both sets of NSC samples compared to both fetal astrocytes.

<p>Transcription factors upregulated in both sets of NSC samples compared to both fetal astrocytes.</p

HOPX, LHX2, and PRRX1 staining of fetal astrocytes.

<p>Lonza and Sciencell fet ast (fetal astrocytes) were stained with HOPX (A), LHX2 (B), and PRRX1 (C) antibodies. The antibody signal is in green and is overplayed with HOECHST (blue) to show all cells.</p

Transcription factors that are expressed in both fetal astrocyte samples and have binding sites within 8-enriched structural genes.

<p>Transcription factors that are expressed in both fetal astrocyte samples and have binding sites within 8-enriched structural genes.</p

Heatmaps for genes differentially expressed growth factor and growth factor receptor genes.

<p>Heatmaps are shown for genes differentially expressed between Lonza fetal astrocytes (LON FET AST), Sciencell fetal astrocytes (SC FET AST), H9 NSCs, and NCRM-5 NSCs. All genes show at least 2-fold difference between at least one fetal astrocyte and NSC sample.</p

Heatmaps for differentially expressed genes from (A) NOTCH, (B) TGF-beta, and (C) JAK-STAT pathways.

<p>Heatmaps are shown for genes differentially expressed between Lonza fetal astrocytes (LON FET AST), Sciencell fetal astrocytes (SC FET AST), H9 NSCs, and NCRM-5 NSCs. All genes show at least 2-fold difference between at least one fetal astrocyte and NSC sample.</p

Transcription factors upregulated in both sets of fetal astrocytes compared to both NSC samples.

<p>Transcription factors upregulated in both sets of fetal astrocytes compared to both NSC samples.</p

GFAP and HOPX expression in NFIX infected fetal astrocytes.

<p>Fetal astrocytes were infected with GFP or NFIX and stained with either (A) GFAP (red), or (B) HOPX (red) antibodies. HOECHST staining (blue) was overplayed to display all cells and GFP (green) to display infected cells.</p

DAPT treatment of NSCs promotes neuronal differentiation.

<p>NSCs were stained for TuJ1 (green) after being grown for four days +/− DAPT in, (A) NSC medium (NSC MED), (B) astrocyte differentiation medium (AST DIFF MED), and (C) neuronal differentiation medium (NEU DIFF MED) with an overlay of HOECHST staining (blue) to display all cells, (D) table quantifying the percentage of TuJ1 positive cells for each treatment group. *p-value = 0.03, **p-value = 0.01, ***p-value = 0.0001.</p