Crocus sativus L. Cultivation in Alpine Environments: Stigmas and Tepals as Source of Bioactive Compounds

Saffron is a well-known spice, produced for a long time, mainly in the Mediterranean area. In the last few years, saffron has been seen as an alternative crop for the diversification of agricultural production and a new source of income, due to its high price, including in Alpine areas. Apart from the spice, constituted by the stigmas, saffron production provides a high amount of fresh tepals, which have so far been considered agricultural waste. Recently, studies on the composition of saffron tepals have been revealing their potential as a rich source of bioactive compounds. In this study, we evaluated the productive traits and the quality of the spice of saffron cultivated in open fields in the north western Italian Alps, for 2 years. In addition, in order to valorize the crop residues, we evaluated the bioactive compound content in tepals by using maceration and ultrasound assisted extraction with different rates of water and methanol as solvents. Higher yields in spice were obtained in the second year of cultivation. However, in both years, the spice had a high quality (ISO 3632), and a very high total phenolic content and antioxidant activity. Thirteen bioactive compounds were identified, including flavonols, cinnamic acids, benzoic acids, catechins, and vitamin C, with few differences between sites and year of cultivation. In tepals, the extraction method and the solvent used influenced the evaluated parameters, i.e., total phenolics and total anthocyanins content, antioxidant activity, and the amount of the four phenolic compounds found (hyperoside, rutin, ellagic acid, and epicatechin). Overall, this study revealed that both saffron spice and its by-product, the fresh tepals, produced in the north western Italian Alps can be considered a source of bioactive compounds with nutraceutical properties, having an antioxidant capacity that is often similar or higher than those of some vegetables and fruits

Environmental Impact of Edible Flower Production: A Case Study

Nowadays the heightened awareness of the critical trend in resource depletion impels to improve the eco − sustainability of any productive process. The research presented in this paper aims to quantify the environmental impact of the emerging productive process of edible flowers, focusing on two model species, i.e., Begonia x semperflorens − cultorum hort and Viola cornuta L., and two types of product, i.e., flowering potted plants sold in plastic vases and packaged flowers ready to be consumed. The study was carried out in an Italian nursery located in Tuscany, interviewing the owners in order to complete the Life Cycle Inventory, assessing the value of the impact categories, and using the “cradle to gate” approach. The information about the production of flowering potted plants and packaged flowers were inserted in a database and elaborated by the appropriate software. The results of the Life Cycle Assessment (LCA) analysis referred to 1 g of fresh edible flowers and were expressed in four impact categories. Global Warming Potential (GWP) values ranged from 24.94 to 31.25 g CO2 eq/g flowers, Acidification Potential (AP) ranged from 8.169E − 02 to 1.249E − 01 g SO2 eq/g flowers, Eutrophication Potential (EP) ranged from 3.961E − 02 to 5.284E − 02 g PO43 − eq/g flowers, and Photochemical Ozone Creation Potential (POCP) ranged from 8.998E − 03 to 1.134E − 02 g C2H4 eq/g flowers. Begonias showed lower emissions than violas in the GWP and POCP indexes, whereas violas showed lower values in the AP and EP impact categories. The most impactful phase was the propagation, accounting on average for 42% of the total emissions. Overall, the findings highlighted a higher environmental load for the production of both begonias and violas packaged flowers, especially if in small containers, rather than as potted plants, with an emission percentage increase from 8% to 17% among the impact categories