The complete mitochondrial genome of <i>Batocera rubus</i> Linnaeus, 1785 (Coleoptera: Cerambycidae)

Batocera rubus severely impacts on the health of banyan trees. In this study, the whole mitochondrial genome for B. rubus was found to be 16,158 bp with a GC content of 23.9%, including 39.1% A, 37.0% T, 14.8% C, and 9.1% G. This genome contains 13 protein-coding genes, 22 tRNAs, and two rRNAs. Phylogenetic analysis revealed that B. rubus is close to Batocera celebiana. This study provides valuable information that can help improve the classification and phylogeny of B. rubus and facilitate further evolutionary studies.</p

MOESM2 of Selection and evaluation of reference genes for qRT-PCR analysis in Euscaphis konishii Hayata based on transcriptome data

Additional file 2. Alignment and phylogenetic tree of 12 candidate genes and 1 validation gene

MOESM1 of Selection and evaluation of reference genes for qRT-PCR analysis in Euscaphis konishii Hayata based on transcriptome data

Additional file 1. Sequences of 12 candidate genes and 1 validation gene

<i>De novo</i> characterization of the pine aphid <i>Cinara pinitabulaeformis</i> Zhang et Zhang transcriptome and analysis of genes relevant to pesticides

<div><p>The pine aphid <i>Cinara pinitabulaeformis</i> Zhang et Zhang is the main pine pest in China, it causes pine needles to produce dense dew (honeydew) which can lead to sooty mold (black filamentous saprophytic ascomycetes). Although common chemical and physical strategies are used to prevent the disease caused by <i>C</i>. <i>pinitabulaeformis</i> Zhang et Zhang, new strategies based on biological and/or genetic approaches are promising to control and eradicate the disease. However, there is no information about genomics, proteomics or transcriptomics to allow the design of new control strategies for this pine aphid. We used next generation sequencing technology to sequence the transcriptome of <i>C</i>. <i>pinitabulaeformis</i> Zhang et Zhang and built a transcriptome database. We identified 80,259 unigenes assigned for Gene Ontology (GO) terms and information for a total of 11,609 classified unigenes was obtained in the Clusters of Orthologous Groups (COGs). A total of 10,806 annotated unigenes were analyzed to identify the represented biological pathways, among them 8,845 unigenes matched with 228 KEGG pathways. In addition, our data describe propagative viruses, nutrition-related genes, detoxification related molecules, olfactory related receptors, stressed-related protein, putative insecticide resistance genes and possible insecticide targets. Moreover, this study provides valuable information about putative insecticide resistance related genes and for the design of new genetic/biological based strategies to manage and control <i>C</i>. <i>pinitabulaeformis</i> Zhang et Zhang populations.</p></div

Putative P450 genes identified in <i>Cinara pinitabulaeformis</i> Zhang et Zhang.

<p>Putative P450 genes identified in <i>Cinara pinitabulaeformis</i> Zhang et Zhang.</p

Number of unigenes related to insecticide receptors and resistance-related genes.

<p>x-axis indicates the number of unigenes, y-axis indicates the specific unigenes related to pesticide receptors and resistance-related genes. One hundred and eighty-seven insecticide receptors and resistance-related unigenes were identified in <i>C</i>. <i>pinitabulaeformis</i> Zhang et Zhang transcriptome.</p

Number of unigenes related to RNAi.

<p>x-axis indicates the corresponding number of unigenes and y-axis indicates the specific unigenes related to RNAi. Thirty-one unigenes coding for RNAi identified in the database.</p

Distribution of second level GO of <i>Cinara pinitabulaeformis</i> Zhang et Zhang transcriptome.

<p>Distribution of GO categories assigned to the <i>Cinara pinitabulaeformis</i> Zhang et Zhang transcriptome. Unigenes were annotated in three categories: cellular components, molecular functions, and biological process. Left y-axis indicates the number of genes in a category.</p

Number of unigenes related to immune-related molecules.

<p>x-axis indicates the corresponding number of unigenes and y-axis indicates the specific unigenes related to immune-related molecules. 478 unigenes were identified related to immune molecules and receptors in the transcriptome. This group contains 20 widely recognized immune factors. <b>(I) Number of specific unigenes related to IMD pathway in immune-related molecules. (II) Number of specific unigenes related to Toll pathway in immune-related molecules</b>. The icon indicates specific unigenes. The number in brackets indicates the corresponding number of unigenes.</p

Number of unigenes related to intestinal digestive enzymes.

<p>x-axis indicates the corresponding number of unigenes and y-axis indicates the specific unigenes related to intestinal digestive enzymes. Three hundred and ninety-four protease-related unigenes were identified in <i>M</i>. <i>alternatus</i> Hope transcriptome. <b>(I) Number of specific unigenes related to serine proteases in intestinal digestive enzymes</b>. The icon indicates specific unigenes. The number in brackets indicates the corresponding number of unigenes.</p