The inhibition curve of some organophosphorus and carbamate pesticides.

<p>The yeast with AChE expression were collected, re-suspended in PBS buffer and seeded in 96-well plate. After incubation with various concentrations of different pesticides, the remaining enzyme activities were determined. Error bars represent the standard deviation of mean values.</p

Primers used in this study.

<p>Primers used in this study.</p

The identification of PCR constructs.

<p>The identification of PCR constructs.</p

Analysis of the growth of yeast and the induced expression of AChE in different culture conditions.

<p>After induction in SC (A) and YPD (B) medium with 1%, 2%, or 4% of galactose for different time periods, the OD₆₀₀ values of yeast suspension were determined. After induction in SC (C) and YPD (D) minimal medium with 1%, 2%, or 4% of galactose for different time periods, the cultures were harvested and the relative enzyme activity of induced AChE was determined. Error bars represent the standard deviation of mean values.</p

The construction of plasmid pYES-DEST52.

<p>The construction of plasmid pYES-DEST52.</p