Comparing Two Different Development Methods of External Parameter Orthogonalization for Estimating Organic Carbon from Field-Moist Intact Soils by Reflectance Spectroscopy

Visible and near-infrared (Vis–NIR) spectroscopy can provide a rapid and inexpensive estimation for soil organic carbon (SOC). However, with respect to field in situ spectroscopy, external environmental factors likely degrade the model accuracy. Among these factors, moisture has the greatest effect on soil spectra. The external parameter orthogonalization (EPO) algorithm in combination with the Chinese soil spectroscopic database (Dataset A, 1566 samples) was investigated to eliminate the interference of the external parameters for SOC estimation. Two different methods of EPO development, namely, laboratory-rewetting archive soil samples and field-collecting actual moist samples, were compared to balance model performance and analytical cost. Memory-based learning (MBL), a local modeling technique, was introduced to compare with partial least square (PLS), a global modeling method. A total of 250 soil samples from Central China were collected. Of these samples, 120 dry ground samples (Dataset B) were rewetted to different moisture levels to develop EPO P1 matrix. Seventy samples (Dataset C) containing field-moist intact and laboratory dry ground soils were used to establish EPO P2 matrix. The remaining 60 samples (Dataset D) also containing field-moist intact and laboratory dry ground soils were employed to validate the spectral models developed based on Dataset A. Results showed that EPO could correct the effect of external factors on soil spectra. For PLS, the validation statistics were as follows: no correction, validation R2 = 0.02; P1 correction, validation R2 = 0.56; and P2 correction, validation R2 = 0.57. For MBL, the validation results were as follows: no correction, validation R2 = 0.06; P1 correction, validation R2 = 0.65; and P2 correction, validation R2 = 0.69. The P2 consistently yielded better results than P1 did but simultaneously increased the sampling time and economic cost. The use of the P1 matrix and the MBL algorithm was recommended because it could reduce the cost of establishing in situ models for SOC

Fixed Spraying Systems Application in Citrus Orchards: Nozzle Type and Nozzle Position Effects on Droplet Deposition and Pest Control

Pesticide application is an essential means of controlling plant diseases and pests in citrus orchards. In recent years, fixed spraying systems have gradually been used as alternatives to traditional sprayers and manual sprayers in some hilly citrus orchards. In this paper, influences of fixed system spraying parameters, such as droplet size and spraying height, on spraying quality were elucidated and analyzed. The performances of two nozzle types, pressure-swirl nozzles and fixed spray plate sprinklers, were assessed and compared by effective droplet coverage ratio (DCR), droplet distribution uniformity coefficient of variation (CV), and droplet penetration ratio (DPR). The results showed that appropriately increasing droplet size and spraying height could improve the DCR and distribution uniformity of pressure-swirl nozzles. The DCR and distribution uniformity of fixed spray plate sprinklers had a positive correlation with droplet size, while spraying height had no significant effect on these variables. Additionally, with the increase in droplet size, DPR initially increased and then gradually decreased. The optimized results showed that the optimal parameters for pressure-swirl nozzles were a droplet size of 240 μm and spraying height of 100 cm, while for fixed spray plate sprinklers, the results were a droplet size of 240 μm and spraying height of 50 cm. Comparison results showed that the spraying quality of fixed spray plate sprinklers was better overall, with values of DCR, CV, and DPR being 37.15%, 24.20%, and 71.67%, respectively, while the corresponding values for pressure-swirl nozzles were 39.65%, 35.41%, and 56.02%. Based on the above results and the occurrence rule of citrus pests and disease, the optimal spraying parameters of fixed spraying systems were selected to control the Asian citrus psyllid Diaphorina citri. Furthermore, the effect of fixed spraying systems on controlling Diaphorina citri reached the maximum at 3 days after spraying, which was 97.83%, and the effect declined at 14 days after spraying, which was 85.47%. This study provides valuable scientific references for guiding the application of fixed spraying systems in hilly citrus orchards

Improving digital mapping of soil organic matter in cropland by incorporating crop rotation

Despite human activities are key influencing factors for cropland soil organic matter (SOM), detailed characterization of human activities has always been limited in the digital mapping of SOM due to the lack of proper representations of human’s cropland use activities. Crop rotation is an essential human agricultural practice significantly affecting the spatial–temporal variations of SOM due to the periodically dynamic changes of crops. Thus, incorporating crop rotation in the digital soil mapping holds high potential for improving SOM prediction. Here, we applied time-series radar Sentinel-1 and optical Sentinel-2 to map crop rotation systems by a hierarchical rule-based method. Then we explored the effectiveness of incorporating such information in predicting SOM by implementing various combinations of predictive variables. We chose a typical multiple cropping region with various crop rotations in southern China. The model performance was evaluated by 10-fold cross-validation. Results showed significant differences in SOM among the crop rotation systems, and the single rice rotated with vegetables has the highest SOM followed by the high-diversity vegetables and long-term orchard systems. Adding crop rotation enhanced the predictability of SOM with a decrease in RMSE by 7% and an increase in R2 by 24%. Furthermore, the crop rotation systems appeared more important in the predictive models than the soil, topographic, and climatic variables. Our results demonstrated the effectiveness of including crop rotation in predicting SOM over complex agricultural landscapes. Our study indicated that human activities should be characterized more detailedly in cropland soil mapping, and that crop rotation containing information on the seasonal dynamics of cropland may be an option for such characterization

Diagnosis of multiple tuberculous muscle abscesses in a patient with systemic lupus erythematosus by metagenomic next-generation sequencing- a case report and literature review

Abstract Background Early diagnosis of muscular tuberculosis (TB) without coexistent active skeletal involvement is often challenging because the disease is very rare and its clinical manifestation is nonspecific and misleading. To raise the awareness and emphasize early diagnosis of muscular TB, we present a case of multiple tuberculous muscle abscesses in a systemic lupus erythematosus (SLE) female, but without pulmonary tuberculosis (PTB), in order to increase awareness of and stress the need of early detection of muscular TB. Case presentation A 44-year-old woman with a 6-year history of SLE who had been treated with methylprednisolone for a long time complained of erythema on her trunk and extremities for five months, along with edema and myalgia for two months, and fever for one month. The patient was first misdiagnosed as SLE overlap dermatomyositis. However, an ultrasound-guided drainage of muscle abscesses revealed positive acid-fast staining combined with positive deoxyribonucleic acid fragment of Mycobacterium tuberculosis using metagenomic next-generation sequencing (mNGS). The patient was cured and released following standard anti-tuberculosis medication, local puncture drainage, and an intravitreal injection of streptomycin. Literature search found only 19 cases of tuberculous muscle abscesses occurring in the extremities reported from 1999 to 2023. Conclusions Extrapulmonary TB with predominantly muscle involvement is rare and with no specific clinical presentation. Muscular tuberculosis may be disdiagnosed for dermatomyositis due to the high muscle enzyme levels, delaying diagnosis and treatment. mNGS technology is helpful in the early and rapid diagnosis of muscular TB. On the basis of traditional anti-tuberculosis treatment, an ultrasound-guided percutaneous puncture drainage and intracavitary injection of streptomycin for the treatment of tuberculous muscle abscess is easy to operate, safe and effective, which is worthy of clinical popularization and application

In Situ Measurements of Organic Carbon in Soil Profiles Using vis-NIR Spectroscopy on the Qinghai–Tibet Plateau

We wish to estimate the amount of carbon (C) stored in the soil at high altitudes, for which there is little information. Collecting and transporting large numbers of soil samples from such terrain are difficult, and we have therefore evaluated the feasibility of scanning with visible near-infrared (vis-NIR) spectroscopy in situ for the rapid measurement of the soil in the field. We took 28 cores (≈1 m depth and 5 cm diameter) of soil at altitudes from 2900 to 4500 m in the Sygera Mountains on the Qinghai–Tibet Plateau, China. Spectra were acquired from fresh, vertical faces 5 × 5 cm in area from the centers of the cores to give 413 spectra in all. The raw spectra were pretreated by several methods to remove noise, and statistical models were built to predict of the organic C in the samples from the spectra by partial least-squares regression (PLSR) and least-squares support vector machine (LS-SVM). The bootstrap was used to assess the uncertainty of the predictions by the several combinations of pretreatment and models. The predictions by LS-SVM from the field spectra, for which <i>R</i>² = 0.81, the root-mean-square error RMSE = 8.40, and the ratio of the interquartile distance RPIQ = 2.66, were comparable to the PLSR predictions from the laboratory spectra (<i>R</i>² = 0.85, RMSE = 7.28, RPIQ = 3.09). We conclude that vis-NIR scanning in situ in the field is a sufficiently accurate rapid means of estimating the concentration of organic C in soil profiles in this high region and perhaps elsewhere

A microporous surface containing Si3N4/Ta microparticles of PEKK exhibits both antibacterial and osteogenic activity for inducing cellular response and improving osseointegration

As an implantable biomaterial, polyetherketoneketone (PEKK) exhibits good mechanical strength but it is biologically inert while tantalum (Ta) possesses outstanding osteogenic bioactivity but has a high density and elastic modulus. Also, silicon nitride (SN) has osteogenic and antibacterial activity. In this study, a microporous surface containing both SN and Ta microparticles on PEKK (STP) exhibiting excellent osteogenic and antibacterial activity was created by sulfonation. Compared with sulfonated PEKK (SPK) without microparticles, the surface properties (roughness, surface energy, hydrophilicity and protein adsorption) of STP significantly increased due to the SN and Ta particles presence on the microporous surface. In addition, STP also exhibited outstanding antibacterial activity, which inhibited bacterial growth in vitro and prevented bacterial infection in vivo because of the presence of SN particles. Moreover, the microporous surface of STP containing both SN and Ta particles remarkably induced response (e.g., proliferation and differentiation) of rat bone mesenchymal stem (rBMS) cells in vitro. Furthermore, STP significantly improved new bone regeneration and osseointegration in vivo. Regarding the induction of cellular response in vitro and improvement of osseointegration in vivo, the microporous surface containing Ta was better than the surface with SN particles. In conclusion, STP with optimized surface properties activated cellular responses in vitro, enhanced osseointegration and prevented infection in vivo. Therefore, STP possessed the dual biofunctions of excellent osteogenic and antibacterial activity, showing great potential as a bone substitute