Showing more kindness to disabled tourists because of understanding: The interaction among the perceived level of information, affection and altruistic behavior intention

It is the common responsibility of the whole society to provide friendly and appropriate help for disabled tourists.However, the altruistic behavior of ordinary tourists towards disabled tourists has been ignored in academia, and few studies have focused on the internal factors that affect the altruistic behavior of ordinary tourists. To fill in these gaps, planning to investigate 500 respondents, based on The Tri-component attitude model, this study, applying SEM, will examine the effect of ordinary tourists\u27 perceived level of information about the travel barriers faced by physical disabled tourists on their empathy and discomfort, which positively affected their altruistic behavior intention to physical disabledtourists. Moreover, the contents of ordinary tourists\u27 perceived level of information and information source will be explored through 10 in-depth interviews. Theoretical and practical implications of this research were discussed as well

Tourists’ green behavior: Co-creation and emotional experience

Based on the theory of inseparability nature of service, service dominant logic, and SOR model, this study examined the interactive relationships among tourists’ co-creation of experience, self-esteem, satisfaction with travel experience, quality of life, and green behavior. Using data collected from 493 tourists in China, the results indicated that co-creation of experience directly influenced their self-esteem, satisfaction with travel experience, and green behavior. Besides, the findings found that emotional experience (satisfaction with travel experience, self-esteem, quality of life) partially mediated the relationships between co-creation experience and green behavior. Finally, co-creation affected tourists’ green behavior through the chain mediating role of self-esteem, satisfaction with travel experience, and quality of life. Theoretical and practical implications were discussed as well

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Design, Synthesis, and Biological Application of Novel Photoaffinity Probes of Dihydropyridine Derivatives, BAY R3401

To explore the molecular mechanisms of BAY R3401, four types of novel photoaffinity probes bearing different secondary tags were synthesized. Their potency for glycogenolysis was evaluated in primary human liver HL-7702 cells and HepG2 cells. Probe 2d showed the best activity in primary human liver HL-7702 cells and HepG2 cells, with IC50 values of 4.45 μM and 28.49 μM, respectively. Likewise, probe 5d showed IC50 values of 6.46 μM in primary human liver HL-7702 cells and 15.29 μM in HepG2 cells, respectively. Photoaffinity labeling experiments were also performed and protein bands larger than 170 kDa were specifically tagged by probe 2d. The results suggest that the synthesized probe 2d might be a very promising tool for the isolation of the target proteins of BAY R3401

In situ observation of the austenite to ferrite transformation in low-carbon steels from different initial phases at defined cooling rates

An experimental investigation has been conducted with respect to the decomposition of austenite to various ferritic products from different initial phase states (molten-state of 1550 °C and solid-state of 1050 °C) and pre-defined cooling rates (1 °C/s and 10 °C/s) in a typical low-carbon steel using the confocal laser scanning microscope (CLSM). Surface morphology, microstructure, internal stress and dislocation density were analyzed. Changing the initial states from the molten to the solid state and lowering the cooling rate from 10 °C/s to 1 °C/s increased the phase transformation starting temperature. Compared with the uniform cooling condition to produce polygonal ferrite of the solid-state sample, unevenly distributed internal stresses were found from the initial molten-state sample. Additionally, high dislocation densities were observed in stress concentrated areas of the initial molten-state samples resulting in the formation of lath-like ferrite but coarse quasi-polygonal ferrite in relatively stress-free areas. Moreover, when the cooling rate was increased to 10 °C/s, the proportion of lath-like ferrite obviously increased in the molten-state and the size of the polygonal ferrite decreased in the solid-state sample due to the increasing of nucleation rate from austenite to ferrite

Optimization of the Alkaline Pretreatment of Rice Straw for Enhanced Methane Yield

The lime pretreatment process for rice straw was optimized to enhance the biodegradation performance and increase biogas yield. The optimization was implemented using response surface methodology (RSM) and Box-Behnken experimental design. The effects of biodegradation, as well as the interactive effects of Ca(OH)2 concentration, pretreatment time, and inoculum amount on biogas improvement, were investigated. Rice straw compounds, such as lignin, cellulose, and hemicellulose, were significantly degraded with increasing Ca(OH)2 concentration. The optimal conditions for the use of pretreated rice straw in anaerobic digestion were 9.81% Ca(OH)2 (w/w TS), 5.89 d treatment time, and 45.12% inoculum content, which resulted in a methane yield of 225.3 mL/g VS. A determination coefficient (R2) of 96% was obtained, indicating that the model used to predict the anabolic digestion process shows a favorable fit with the experimental parameters

Design, Synthesis, and Use of Novel Photoaffinity Probes in Measuring the Serum Concentration of Glycogen Phosphorylase

A procedure to measure the serum concentration of glycogen phosphorylase during acute myocardial infarction is presented. This method was based on the synthesis of photoaffinity probes, and used the semiquantitative protein electrophoretic mobility shift technique. Three novel photoaffinity probes bearing different secondary tags were synthesized. Their potency was evaluated in an enzyme inhibition assay against rabbit muscle glycogen phosphorylase a (RMGPa). The inhibitory activity of probe 1 was only 100-fold less potent than the mother compound CP-320626. The photoaffinity labeling experiments were also performed, and a protein with molecular weight (MW) of about 90⁻100 kDa, which was consistent with the MW of GP, was clearly labeled by probe 1. A semiquantitative evaluation of the GP level in serum with probe 1 was also performed. The results showed that the protein band with a MW of about 90⁻100 kDa was tagged, and the concentration of the protein in serum was found to be between 25 and 50 ng/mL. Mass spectrometric analysis revealed that alpha-1,4 glucan phosphorylase (GPMM) was well-preserved in the bands

Comparative Transcriptomic Profiling in Ovarian Tissues of Lohmann Hens and Chengkou Mountain Chicken

Background: As a crucial economic characteristic and a major indicator of reproductive performance in layers, egg production is controlled by a series of complex regulatory heredity basis. In particular, the interacting regulatory function between noncoding RNAs (ncRNAs) and coding RNA plays important roles in regulating laying performance. Methods: In this study, the RNA sequencing (RNA-seq) of ovarian tissues from Lohmann hens (n = 3) and Chengkou Mountain chicken (n = 3) under the laying peak period was performed to identify RNA transcriptional differences among different laying-performance populations. Results: Results showed that the expression level of 303 mRNAs, 68 long ncRNAs (lncRNAs), 533 circular RNAs (circRNAs), and 79 microRNAs (miRNAs) was significantly different among the groups. Functional enrichment analysis of these differentially expressed (DE) mRNAs revealed that the laying process was implicated in numerous significantly enriched pathways (p < 0.05), such as the neuroactive ligand-receptor interaction, steroid hormone biosynthesis, and calcium-signaling pathway. Furthermore, the lncRNA/circRNA–miRNA–mRNA regulatory networks related to the regulation of laying performance were constructed. Some randomly selective DE RNAs were verified by Real Time Quantitative (RT-qRCR), indicating that the bioinformatics analysis results of RNA-seq data were credible. Conclusions: This study could increase our understanding of the heredity basis of transcriptome in the laying performance of chicken

Application of global phase filtering method in multi frequency measurement

In reverse engineering, reconstruction of 3D point cloud data is the key step to acquire the final profile of the object. However, the quality of 3D reconstruction is influenced by noise in the three-dimensional measurement. This paper aims to tackle the issue of removing the noisy data from the complex point cloud data. The 3D-GPF (Three Dimensional Global Phase Filtering) global phase filtering method is proposed based on the study of phase filtering method, consisting of the steps below. Firstly, the six-step phase shift profilometry is used to obtain the local phase information, and encoding the obtained phase information. Through the global phase unwrapping method, the global phase can be acquired. Secondly, 3D-GPF method is used for the obtained global phase. Finally, the effect of 3D reconstruction is analyzed after the global phase filtering. Experimental results indicate that the noisy points of three-dimensional graphics is reduced 98.02%, the speed of 3D reconstruction is raised 12%.The effect of the proposed global phase filtering method is better than DCT and GSM methods. It is high precision and fast speed, and can be widely used in other 3D reconstruction application

A color phase shift profilometry for the fabric defect detection

For fabric defect identification in the textile industry, a three-dimensional (3D) color phase shift profilometry (CPSP) method is proposed. The detecting system is mainly composed of one CCD camera and one digital-light-processing (DLP) projector. Before detection, the system should be calibrated to make sure the camera parameters. The CPSP color grating is projected to the measured fabric by DLP projector, and then it is collected by CCD camera to obtain the grating phase. The 3D measurement can be completed by the grating phase difference. In image acquisition, only invariable grating is projected to the object. In order to eliminate the interference from background light during the image acquisition, the brightness correction method is researched for improving the detection accuracy. The experimental results show that the false rate of detecting the fabric defects is 5.78%, the correct rates of detecting the fabric defects of hole and qualified fabric are both 100%, and the correct rates of detecting the fabric defect of scratch and fold are 98% and 96%, respectively. The experiment proves that the proposed method can accurately identify fabric defects