A service plan for JobCity.Net: A division of TAO Solutions Inc.

Jobscity. Net is the head hunting division of the company Tao Solutions Inc. Aside from JobsCity.Net Tao Solutions Inc or TSI has three other business division, Almuni. Net, TravelSmart.Net, and SmartShopper.Net. JobsCity.Net first started with job-matching website in 1999 and then later on began its head hunting operations in 2005. The company division specializes in the staffing of BPO companies providing IT and ITES. The division is still a small player in the head hunting industry compared to other big companies like John Clements which has been in the industry for almost four decades. The growth of the head hunting industry in the Philippines is directly related to the growth of the BPO industry in the Philippines. As the BPO industry in the Philippines becomes larger and larger, the more these companies would require outsourcing their staffing needs. With the increase in the number of BPO companies in the Philippines and current situations occurring in different parts of the globe, the company is presented with opportunities where in it should utilize its resources to cater to the ongoing needs of the business processes outsourced in the country. The goal of the paper is to improve the current performance of the company through the group\u27s proposed strategies and programs. The suggested strategies and programs are in line with the company\u27s goals for the coming year. The goal of the group is to focus first on the company division\u27s secondary target market which is the individual market. The group believes that by increasing the number of applicants applying at the company and by improving their service of providing qualified agents for their current clients will pave the way in increasing the company\u27s revenue. The marketing mix of this plan includes promotional schemes, print advertising, modifications in their current offerings, and non traditional advertising schemes, all for the purpose of attracting more applicants and clients, thus more business opportunities. Estimated cost of the proposed plan will total about P2,202,100 with an expected net profit of P1,091,320 at the end of the second year. All the figures in Table 5 and Table 6 are based on conservation estimates. Evaluation of the proposed plan will be done through the help of Interview Assessment Sheet and by monitoring the ratio of the number of endorsements to the number of hires which is based by the group on the industry average to be five (5) endorsements is to one (1) hire. With the implementation of the groups proposals of free call center workshop, the ratio is expected to improve to four (4) is to one (1)

Proliferative endocrine effects of adipose tissue from obese animals on MCF7 cells are ameliorated by resveratrol supplementation.

Obesity is clearly associated with an increased risk of breast cancer in postmenopausal women. The purpose was to determine if obesity alters the adipocyte adipokine secretion profile, thereby altering the adipose-dependent paracrine/endocrine growth microenvironment surrounding breast cancer cells (MCF7). Additionally, we determined whether resveratrol (RSV) supplementation can counteract any obesity-dependent effects on breast cancer tumor growth microenvironment. Obese ZDF rats received standard chow diet or diet supplemented with 200 mg/kg body weight RSV. Chow-fed Zucker rats served as lean controls. After 6 weeks, conditioned media (CM) prepared from inguinal subcutaneous adipose tissue (scAT) was added to MCF7 cells for 24 hrs. Experiments were also conducted using purified isolated adipocytes to determine whether any endocrine effects could be attributed specifically to the adipocyte component of adipose tissue. scAT from ZDF rats promoted cell cycle entry in MCF7 cells which was counteracted by RSV supplementation. RSV-CM had a higher ratio of ADIPO:LEP compared to ZDF-CM. This altered composition of the CM led to increased levels of pAMPKT172, p27, p27T198 and AdipoR1 while decreasing pAktT308 in MCF7 cells grown in RSV-CM compared to ZDF-CM. RSV-CM increased number of cells in G0/G1 and decreased cells in S-phase compared to ZDF-CM. Co-culture experiments revealed that these obesity-dependent effects were driven by the adipocyte component of the adipose tissue. Obesity decreased the ratio of adiponectin:leptin secreted by adipocytes, altering the adipose-dependent growth microenvironment resulting in increased breast cancer cell proliferation. Supplementation with RSV reversed these adipose-dependent effects suggesting a potential for RSV as a nutritional supplementation to improve breast cancer treatment in obese patients

ZDF rats supplemented with RSV prevents the deleterious effects of ZDF-CM on cell cycle proteins.

<p>A: Representative western blots for selected proteins showing the effects of treatment with CM prepared from UT (open bar), ZDF-CM (closed bar), ZDF+RSV-CM (grey bar) and lean Zucker-CM (hatched bar) on proliferating MCF7 cells. B-F: Graphical representations of multiple experiments showing the effects of CM on pAMPK^T172, pAKT^T308, p27, p27^T198 and AdipoR1 protein levels. β-actin was used as a loading control. One-way ANOVAs showed significant main effects for all experiments (p<0.05). * denotes significantly different from ZDF (p<0.05) as determined by Tukey’s post-hoc tests; ** indicates significantly different from all other groups (p<0.05; n = 5-6/group).</p

Lean Zucker subcutaneous adipocytes co-culture positively affects MCF7 cell cycle proteins in does-dependent manner.

<p>A: Representative western blots for selected proteins showing the effects of treatment with UT (open bar) or lean Zucker scAT increasing adipocyte number (closed bars) or the highest adipocyte number (4.4×10⁶) plus ADIPO (18 nM; grey bar) co-culture treatment for 24 hours in arrested MCF7 cells. B-F: Graphical representations of multiple experiments showing the effects of lean Zucker scAT increasing adipocyte number and the addition of ADIPO (9 nM) on pAMPK^T172, pAKT^T308, p27, p27^T198 and AdipoR1 protein levels. β-actin was used as a loading control. One-way ANOVAs showed significant main effects for all experiments (p<0.05). * denotes significantly different from ZDF (p<0.05) as determined by Tukey’s post-hoc tests (n = 5/group).</p

Co-cultured subcutaneous adipocytes elicit cell cycle changes in MCF7 cells.

<p>A: Graphical representation of ADIPO and LEP concentration (ng/ml) in media co-cultured with isolated subcutaneous adipocytes and MCF7 cells. B-C: Typical cell cycle profiles in MCF7 cells treated with lean Zucker adipocytes (4.4×10⁶) and ZDF adipocytes (1.6×10⁵). D: Graphical representation of multiple cell cycle profile experiments observing effects of adipocyte co-culture with ZDF (closed bars), ZDF+ 9 nM ADIPO (grey bars) and lean Zucker (hatched bars) in arrested MCF7 cells. One-way ANOVAs showed significant main effects for all experiments (p<0.05). * denotes significantly different from ZDF (p<0.05) as determined by Tukey’s post-hoc tests (n = 5/group).</p

ZDF+RSV-CM supplementation causes cell cycle exit in MCF7 cells compared to ZDF-CM.

<p>A: Graphical representation of ADIPO and LEP concentration (ng/ml) in conditioned media (CM) created from scAT. B-C: Typical cell cycle profiles in MCF7 cells grown in CM prepared from scAT of ZDF or ZDF+RSV supplemented rats. D: Graphical representation of multiple cell cycle profile experiments observing effects of ZDF-CM (closed bars), ZDF+RSV-CM (grey bars) and lean Zucker (hatched bars) in proliferating MCF7 cells. * denotes significantly different from ZDF (p<0.05) as determined by Tukey’s post-hoc tests (p<0.05, n = 5-6/group).</p