Characterization of soluble TLR2 and CD14 levels during acute dengue virus infection

Dengue virus infection results in a broad spectrum of diseases ranging from mild dengue fever (DF) to severe dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Hitherto, there is no consensus biomarker for the prediction of severe dengue disease in patients. Yet, early identification of patients who progress to severe dengue is pivotal for better clinical management. We have recently reported that an increased frequency of classical (CD14 ++CD16 -) monocytes with sustained high TLR2 expression in acutely infected dengue patients correlates with severe dengue development. Here, we hypothesized that the relatively lower TLR2 and CD14 expression in mild dengue patients is due to the shedding of their soluble forms (sTLR2 and sCD14) and that these could be used as indicators of disease progression. Therefore, using commercial sandwich ELISAs, we evaluated the release of sTLR2 and sCD14 by peripheral blood mononuclear cells (PBMCs) in response to in vitro dengue virus (DENV) infection and assessed their levels in acute-phase plasma of 109 dengue patients. We show that while both sTLR2 and sCD14 are released by PBMCs in response to DENV infection in vitro, their co-circulation in an acute phase of the disease is not always apparent. In fact, sTLR2 was found only in 20% of patients irrespective of disease status. In contrast, sCD14 levels were detected in all patients and were significantly elevated in DF patients when compared to DHF patients and age-matched healthy donors. Altogether, our results suggest that sCD14 may help in identifying patients at risk of severe dengue at hospital admittance. </p

Microcredit and savings associations for building rural household resilience: A case study of selected village development fund and savings groups in Koh Kong and Mondul Kiri, Cambodia

This case study was conducted to generate insights on the financial sustainability of selected VDFSGs and to gather information on members’ perceptions of the usefulness of these institutions in coping with household and climate change-related shocks or stresses. Financial sustainability was analyzed by conducting a detailed financial analysis of six selected VDFSGs to determine the sufficiency of interest payments as revenue to cover total costs as well as to evaluate loan recovery and equity build- up. Members’ perception of the usefulness of VDFSGs in helping them to cope with and adjust to family and climate change-related shocks/stresses was determined by conducting Focus Group Discussions (FGDs) and Key Informant Interviews (KIIs) among selected representatives of VDFSG members. Useful feedback of the financial performance and areas for improvement were generated. The Pu Hong, Pu Chhob, and Prek Svay VDFSGs were considered financially sustainable based on the results of the study. The study also revealed that the VDFSGs are considered most useful when there are crop failures due to extreme weather events and when there are medical emergencies in the household. The FGD participants and key informants expressed confidence that they are in a better position to cope with their vulnerabilities due to the presence of a VDFSG in their village

The Effectiveness of Contract Farming for Raising Income of Smallholder Farmers in Low- and Middle-Income Countries: a Systematic Review

Contract farming is used by an increasing number of firms as a preferred modality to source products from smallholder farmers in low and middle-income countries. Quality requirements of consumers, economies of scale in production or land ownership rights are common incentives for firms to offer contractual arrangements to farmers. Prices and access to key technology, key inputs or support services are the main incentives for farmers to enter into these contracts. There is great heterogeneity in contract farming, with differences in contracts, farmers, products, buyers, and institutional environments. The last decade shows a rapid increase in studies that use quasi-experimental research designs to assess the effects of specific empirical instances of contract farming on smallholders. The objective of this systematic review was to distill generalised inferences from this rapidly growing body of evidence. The review synthesised the studies in order to answer two questions: 1: What is known about the effect size of contract farming on income and food security of smallholder farmers in low- and middle-income countries? 2: Under which enabling or limiting conditions are contract farming arrangements effective for improving income and food security of smallholders