Fluorescence micrographs showing two successive rounds of vesicle fusion (mixing), reaction, and budding/fission (aliquoting).

<p>The 5-µm scale bar in panel (a) also applies to panel (b) and to panels (e)–(h), whereas the 10-µm scale bar in panel (c) also applies to panel (d).</p

Brightfield micrographs showing the fusion, budding, and fission processes of GUVs.

<p>Fission (complete detachment) of daughter GUVs after budding was induced by lowering the temperature close to the <i>T_m</i> of the lipids.</p

Histograms of the M-m ratio for different concentrations of PEG lipid micelles.

<p>(a) 0 <i>μ</i>M, (b) 2.58 <i>μ</i>M, (c) 5.16 <i>μ</i>M, (d) 10.32 <i>μ</i>M, (e) 20.63 <i>μ</i>M, (f) 41.25 <i>μ</i>M, (g) 82.5 <i>μ</i>M, and (h) 165 <i>μ</i>M. Characteristic peaks were indicated as arrows.</p

A schematic for vesicle image analysis.

<p>Vesicles are reacted with PEG lipid micelles to induce shape transformations and images are taken by a confocal microscope. Each vesicle image was binarized separately and approximated with an ellipsoid to measure the lengths of major and minor axes.</p

Histograms of the M-m ratio for different concentrations of PEG lipid micelles.

<p>(a) 0 <i>μ</i>M, (b) 2.58 <i>μ</i>M, (c) 5.16 <i>μ</i>M, (d) 10.32 <i>μ</i>M, (e) 20.63 <i>μ</i>M, (f) 41.25 <i>μ</i>M, (g) 82.5 <i>μ</i>M, and (h) 165 <i>μ</i>M. Characteristic peaks were indicated as arrows.</p

Temporal changes of the mean and variance of the M-m ratio.

<p>Temporal changes of the mean and variance of the M-m ratio.</p

A shape transformation of lipid vesicle induced by PEG lipid micelles.

<p>(a) A spherical vesicle transformed into (b) a disc-like oblate, (c) cylindrical prolate, and eventually divided into (d) two large and small vesicles.</p

The review of the National Curriculum in England The Secretary of State's proposals

Consultation period May-July 1999SIGLEAvailable from British Library Document Supply Centre-DSC:f99/3455 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Flow selector device.

<p>(A) Schematic diagram of 3D printed flow selector device using PDMS membrane valve. (inset) Valves are closed when air pressure is applied from the bottom channel. When they are open, aqueous phases pumped at a constant pressure flow through the valve. (B) Fabricated flow selector device. (C) Plot of blue dye level measured at the outlet of the device, indicated as dotted square in (B). Deionized water with and without blue dye were switched every 15s.</p