Relationships between FMISO hypoxic lesion volume, MRI lesion volume and TTC lesion volume.

<p>Each plot uses data from both SHR and Wistar rats under both the control and hyperoxia conditions (n = 13). All Pearson correlations are statistically significant (p < 0.001; R² values shown next to each scatterplot).</p

Mean (± SD) FMISO PET hypoxic volume and MRI and TTC lesion volumes for both rat strains and oxygen supply conditions, together with corresponding t and p values from two-sample, two-tailed, unequal variance t-tests between control and hyperoxia oxygen conditions.

<p>Mean (± SD) FMISO PET hypoxic volume and MRI and TTC lesion volumes for both rat strains and oxygen supply conditions, together with corresponding t and p values from two-sample, two-tailed, unequal variance t-tests between control and hyperoxia oxygen conditions.</p

Illustrative coregistered FMISO SUV, DWI and T2-weighted MRI images for the same coronal cut in one spontaneously hypertensive rat and one Wistar rat, and approximately matched TTC coronal slice.

<p>These data illustrate the topographic congruence of the ischemic lesions (arrows) among the four imaging modalities. As TTC sections are not amenable to coregistration with PET and MR images due to differences in slice angle and thickness and potential <i>post-mortem</i> geometrical distortion, approximately same coronal level TTC sections as for the <i>in vivo</i> images are shown for illustrative purposes only.</p

Individual cortical PO₂ (P_tO₂) measured in the MCA territory in the non-ischemic hemisphere (left) and ischemic hemisphere (right) under the control and hyperoxia conditions in SHR (top) and Wistar rats (bottom; n = 5 and 4, respectively).

<p>Each line joins P_tO₂ values obtained under both conditions for a single rat; different colors are used for ease of illustration. The inset on the top right zooms on the four SHRs with lowest baseline P_tO₂ values, which are difficult to discriminate on the main graph due to the common scale used across graphs. This figure shows lower P_tO₂ under ischemic vs control condition in both strains, and increases in P_tO₂ under hyperoxia in all 9 rats (although of very small amplitude in one SHR), which was highly statistically significant with pooled data. However, due to small samples and highly variable P_tO₂ increases, only trends were found when strains were assessed separately. The increase in P_tO₂ by hyperoxia was not significantly different between the two strains in the ischemic cortex, although was significantly higher for Wistar rats in the contralateral hemisphere. Statistical tests also showed significantly higher P_tO₂ in the control condition in Wistar rats as compared to SHRs in both hemispheres (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0187087#sec021" target="_blank">Results</a> for further details).</p

Representative FMISO time-activity curves for the FMISO hypoxic lesion ROI and contralateral ROI for the 3-hr PET scan, together with compartmental fits for SHR (top row) and Wistar (bottom row) rats under the control (left column) and hyperoxia conditions (right column).

<p>Representative FMISO time-activity curves for the FMISO hypoxic lesion ROI and contralateral ROI for the 3-hr PET scan, together with compartmental fits for SHR (top row) and Wistar (bottom row) rats under the control (left column) and hyperoxia conditions (right column).</p

Representative FMISO SUV images overlaid on the same coronal slice of the co-registered MRI template for representative SHR (top row) and Wistar (bottom row) rats scanned under either the control (left column) or hyperoxia (right column) conditions.

<p>The pseudocolor scale on the right represents SUV units (see text for details). This illustrates the much smaller FMISO lesion induced by distal MCA occlusion in Wistar rats than SHRs, and the lack of clear effect of hyperoxia on FMISO brain uptake in either strain (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0187087#sec021" target="_blank">Results</a> for further details).</p