1 research outputs found
Detection of the Lipopeptide Pam3CSK4 Using a Hybridized Toll-like Receptor Electrochemical Sensor
Electrochemical detection
of Pam3CSK4, a synthetic triacylated
lipopeptide that mimics the structural moieties of its natural Gram
negative bacterial pathogen-associated molecular pattern (PAMP) counterpart,
has been achieved using hybridized toll-like receptors (TLR) combining
TLR1 and TLR2 onto a single sensor surface. These sensors represent
the first hybridized TLR sensors. The limit of detection for Pam3CSK4
attained was 7.5 μg/mL, which is within the same order of magnitude
for that of the more labor-intensive and time-consuming cell-assay
technique, 2.0 μg/mL. The results gathered in these electrochemical
experiments show that sensors fabricated by immobilizing a mixture
of cooperative TLR1 and -2 generate higher responses when exposed
to the analyte in comparison to the control sensors fabricated using
pure TLR1 or -2 standalone. A PAMP selectivity test was carried out
in line with our inspiration from the mammalian innate immune response.
TLRs1–5 as standalone biorecognition elements and the hybridized
“TLR1 and 2” sensor surface were investigated, understanding
the known TLR-PAMP interactions, through the exploitation of this
electrochemical sensor fabrication technique. The experimental result
is consistent with observations from previously published <i>in vivo</i> and <i>in vitro</i> studies, and it is
the first demonstration of the simultaneous evaluation of electrochemical
responses from multiple, unique fabricated TLR sensor surfaces against
the same analyte