Differentiation of 288 <i>G</i>. <i>barbadense</i> accessions based on genetic and principal component analysis.

<p>Differentiation of 288 <i>G</i>. <i>barbadense</i> accessions based on genetic and principal component analysis.</p

Correlations between fiber traits of <i>G</i>. <i>barbadense</i> cultivars in the Tashkent and California environments.

<p>Correlations between fiber traits of <i>G</i>. <i>barbadense</i> cultivars in the Tashkent and California environments.</p

Summary of SSR markers showed significant association with each of the trait studied in the Uzbekistan (UZB) and USA environments.

<p>Summary of SSR markers showed significant association with each of the trait studied in the Uzbekistan (UZB) and USA environments.</p

Principal component analysis, of 288 <i>G</i>. <i>barbadense</i> cultivars in the space of two main coordinate jointly by SSR genotypes.

<p>PC—the main components; (A) and (B)—subgroups represented in the majority of varieties of Uzbekistan (UZ) and Turkmenistan (TM), respectively. (Mix)—represented by the most genetically differentiated samples from several geographic regions i.e., from Turkmenistan (8), Africa (3), Uzbekistan (3), and American (1). UZ—Uzbekistan, TM—Turkmenistan, TJ—Tajikistan, AF—Africa, US—US, SA—South America AZ—Azerbaijan and ME—Middle East.</p

Genetic diversity, linkage disequilibrium, and association mapping analyses of <i>Gossypium barbadense</i> L. germplasm

<div><p>Limited polymorphism and narrow genetic base, due to genetic bottleneck through historic domestication, highlight a need for comprehensive characterization and utilization of existing genetic diversity in cotton germplasm collections. In this study, 288 worldwide <i>Gossypium barbadense</i> L. cotton germplasm accessions were evaluated in two diverse environments (Uzbekistan and USA). These accessions were assessed for genetic diversity, population structure, linkage disequilibrium (LD), and LD-based association mapping (AM) of fiber quality traits using 108 genome-wide simple sequence repeat (SSR) markers. Analyses revealed structured population characteristics and a high level of intra-variability (67.2%) and moderate interpopulation differentiation (32.8%). Eight percent and 4.3% of markers revealed LD in the genome of the <i>G</i>. <i>barbadense</i> at critical values of r² ≥ 0.1 and r² ≥ 0.2, respectively. The LD decay was on average 24.8 cM at the threshold of r² ≥ 0.05. LD retained on average distance of 3.36 cM at the threshold of r² ≥ 0.1. Based on the phenotypic evaluations in the two diverse environments, 100 marker loci revealed a strong association with major fiber quality traits using mixed linear model (MLM) based association mapping approach. Fourteen marker loci were found to be consistent with previously identified quantitative trait loci (QTLs), and 86 were found to be new unreported marker loci. Our results provide insights into the breeding history and genetic relationship of <i>G</i>. <i>barbadense</i> germplasm and should be helpful for the improvement of cotton cultivars using molecular breeding and omics-based technologies.</p></div

The UPGMA dendrogram of 288 <i>G</i>. <i>barbadense</i> accessions, constructed using the genotype of 301 polymorphic SSR alleles.

<p>Horizontal lines denote thresholds of genetic distances. Groups A and B are obtained on the basis of differences in > 50%, whereas subgroups G1, G2 and G3 obtained based on the upper boundary distinctions in 40%, and the subgroups G5 and G4—the upper bound of 20%.</p

Pair-wise comparisons of F_st values specific to each ecotype.

<p>Pair-wise comparisons of F_st values specific to each ecotype.</p

A distribution of alleles among the 108 SSR markers.

<p>A distribution of alleles among the 108 SSR markers.</p

Result of association mapping of fiber quality traits in a particular region.

<p>Markers showed signisicant association (MLM; <i>p</i> ≤0.05) both in Uzbekistan (Uzb.), and the United States (US) environments.: FL-fiber length, FM- micronaire, FS- fiber strength, FU- uniformity.</p