Physical Exercise After Solid Organ Transplantation: A Cautionary Tale

An increasing body of randomized controlled trials suggests the safety of engaging in moderate to vigorous intensity exercise training following solid organ transplantation. Fueled by emerging sport events designed for transplant recipients and the ever-growing body of research highlighting the diverse health benefits of physical activity, transplant recipients are now increasingly participating in strenuous and occasionally competitive physical endeavors that largely surpass those evaluated in controlled research settings. This viewpoint article adopts a cautionary stance to counterbalance the prevalent one-sided optimistic perspective regarding posttransplant physical activity. While discussing methodological limitations, we explore plausible adverse impacts on the cardiovascular, immunological, and musculoskeletal systems. We also examine the physiological consequences of exercising in the heat, at high altitude, and in areas with high air pollution. Risks associated with employing performance-enhancing strategies and the conceivable psychological implications regarding physical activity as a tribute to the ‘gift of life’ are discussed. With a deliberate focus on the potential adverse outcomes of strenuous posttransplant physical activity, this viewpoint aims to restore a balanced dialogue on our comprehension of both beneficial and potentially detrimental outcomes of physical activity that ultimately underscores the imperative of well-informed decision-making and tailored exercise regimens in the realm of posttransplant care

Multimycotoxin UPLC-MS/MS for tea, herbal infusions and the derived drinkable products

In recent years the consumption of tea and herbal infusions has increased. These hot drinks are consumed as daily drinks as well as for medicinal purposes. All tea varieties (white, yellow, green, oolong, black and puerh) originate from the leaves of the tea plant, Camellia sinensis. All extracts made of plant or herbal materials which do not contain Camellia sinensis are referred as herbal infusions or tisanes. During processing and manufacturing fungal contamination of the plant materials is possible, enabling contamination of these products with mycotoxins. In this study a multimycotoxin UPLC-MS/MS method was developed and validated for the analysis of the raw tea and herbal infusion materials as well as for their drinkable products. The samples were analyzed by ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), with a mobile phase consisting of variable mixtures of water and methanol with 0.3% formic acid. The limits of detection for the different mycotoxins varied between 2.1 mu g/kg and 121 mu g/kg for raw materials and between 0.4 mu g/L and 46 mu g/L for drinkable products. Afterward 91 different tea and herbal infusion samples were analyzed. Only in one sample, Ceylon melange, 76 mu g/kg fumonisin B-1 was detected. No mycotoxins were detected in the drinkable products

Production and migration of mycotoxins in sweet pepper analyzed by Multimycotoxin LC-MS/MS

In this work the presence and migration behavior of mycotoxins formed in sweet pepper, inoculated by Fusarium species involved in internal fruit rot, were investigated. Two different commercial sweet pepper cultivars were inoculated with two different Fusarium proliferatum isolates that were sampled from diseased peppers. After 10 days of incubation at 20 degrees C in a closed container, the lesion caused by the fungal infection was dissected. Around the lesion, up to three concentric rings of pepper fruit tissue with a width of 5 mm were cut out and analyzed using a multimycotoxin LC-MS/MS method. The analyses resulted in the detection of beauvericin and fumonisins B-1, B-2, and B-3. Beauvericin was detected only in the lesions (95%), and the levels varied between 67 and 73800,mu g/kg. Fumonisins B-1, B-2, and B-3 were detected in the lesions and in the surrounding tissue, indicating migration of these toxins into healthy parts of the sweet pepper. In the lesion the fumonisin B-1 level varied between 690 and 104000 mu g/kg. Even in the outer ring fumonisin B-1 was still present. Mostly it was present at a lower level than in the lesion, with a maximum level of 556 mu g/kg. A similar migration behavior was obtained for fumonisins B-2 and B-3, but lower levels were detected in the lesions, up to 10900 and 1287,mu g/kg, respectively. The analysis of 20 pepper samples resulted in the detection of beauvericin or alternariol. Seven samples were contaminated, and the level of beauvericin was 124 mu g/kg (N = 1), whereas the level of alternariol varied from below the LOQ (6.6 mu g/kg) to 101 mu g/kg (N = 6)

A validated multianalyte LC-MS/MS method for quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples

This study was designed to develop a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous detection and quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples with particular focus on the optimization of the sample preparation protocol and method validation. All 25 mycotoxins were extracted in a single step with a mixture of methanol/ethyl acetate/water (70:20:10, v/v/v). The method limits of quantification (LOQ) varied from 0.3 mu g/kg to 106 mu g/kg. Good precision and linearity were observed for most of the mycotoxins. The method was applied for the analysis of naturally contaminated peanut cake, cassava flour and maize samples from the Republic of Benin. All samples analyzed (fifteen peanut cakes, four maize flour and four cassava flour samples) tested positive for one or more mycotoxins. Aflatoxins (total aflatoxins; 10-346 mu g/kg) and ochratoxin A (<LOQ-2 mu g/kg) were detected in peanut cake samples while fumonisin B(1) (4-21 mu g/kg), aflatoxin B(2) (<LOQ-8 mu g/kg), aflatoxin B(1) (<LOQ-9 mu g/kg), diacetoxyscirpenol (<LOQ-6 mu g/kg) and zearalenone (<LOQ-12 mu g/kg) were detected and quantified in cassava flour samples. Fumonisin B(1) (13-836 mu g/kg), fumonisin B(2) (5-221 mu g/kg), fumonisin B(3) (<LOQ-375 mu g/kg) and beauvericin (<LOQ-25 mu g/kg) were detected in the maize samples

Genetic diversity and mycotoxin production of Fusarium lactis species complex isolates from sweet pepper.

An internal fruit rot disease of sweet peppers was first detected in Belgium in 2003. Research conducted mostly in Canada indicates that this disease is primarily caused by Fusarium lactis Pirotta. Ninety-eight Fusarium isolates obtained from diseased sweet peppers from Belgium, as well as from other countries (Canada, the Netherlands and the United Kingdom) were identified by sequencing the translation elongation factor 1α (EF). Of these 98 isolates, 13 were identified as F. oxysporum Schltdl., nine as F. proliferatum (Matsush.) Nirenberg and two belonged to clade 3 of the F. solani species complex. Of the 74 remaining isolates, the EF sequence showed 97% to 98% similarity to F. lactis. Of these isolates, the β-tubulin (TUB), calmodulin (CAM) and the second largest subunit of RNA polymerase II (RPB2) genes were also sequenced. Analysis of the combined sequences revealed that the 74 isolates share nine combined sequences that correspond to nine multilocus sequence types (STs), while the F. lactis neotype strain and one other strain, both isolated from figs, form a separate ST. Together, these 10 STs represent a monophyletic F. lactis species complex (FLASC). An unusually high level of genetic diversity was observed between (groups of) these STs. Two of them (ST5 and ST6) fulfilled the criteria for species recognition based on genealogical exclusivity and together represent a new monophyletic species lineage (FLASC-1). The seven other STs, together with the F. lactis neotype ST, form a paraphyletic species lineage in the African clade of the Gibberella fujikuroi species complex (GFSC). From each of the 10 STs, the mycotoxin production was assessed using a multi-mycotoxin liquid chromatography mass spectrometry method. Out of the 27 analyzed mycotoxins, beauvericin and fumonisins were detected in sweet pepper tissue and in maize kernels. The 10 STs clearly differed in the amount of mycotoxin produced, but there was only limited congruence between the production profile and the phylogenetic analysis. Furthermore, the morphological characterization (based on mycelial growth rate and the length of macroconidia) showed distinct differences between the 10 STs, but again there was limited congruence with the phylogenetic results. In conclusion, the data presented in this study demonstrate that 75% of the isolates obtained from sweet pepper with internal fruit rot belong to a F. lactis species complex (FLASC), including a new FLASC-1 monophyletic species, and that the members of this complex display great genetic and phenotypic diversity