1 research outputs found
UPLC-ESI-TOF MS-Based Metabolite Profiling of the Antioxidative Food Supplement Garcinia buchananii
Comparative antioxidative analyses
of aqueous ethanolic extracts
from leaf, root, and stem of Garcinia buchananii revealed high activity of all three organs. To investigate the metabolite
composition of the different parts of <i>G. buchananii</i>, an untargeted metabolomics approach using UPLC-ESI-TOF MS with
simultaneous acquisition of low- and high-collision energy mass spectra
(MS<sup>e</sup>) was performed. Unsupervised statistics (PCA) highlighted
clear differences in the metabolomes of the three organs. OPLS-DA
revealed (2<i>R</i>,3<i>S</i>,2″<i>R</i>,3″<i>R</i>)-GB-1, (2<i>R</i>,3<i>S</i>)-morelloflavone, and (2<i>R</i>,3<i>S</i>)-volkensiflavone as the most decisive marker compounds
discriminating leaf from root and stem extract. Leaves represent the
best source to isolate GB-1, morelloflavone, and volkensiflavone.
Root extract is the best organ to isolate xanthones and stem bark
extract the best source to isolate (2<i>R</i>,3<i>S</i>,2″<i>R</i>,3″<i>R</i>)-manniflavanone;
the identified polyisoprenylated benzophenones are characteristic
compounds for the leaf organ. Morelloflavone, volkensiflavone, and
garcicowin C were isolated for the first time from <i>G. buchananii</i>, identified via MS, NMR, and CD spectroscopy, and showed in H<sub>2</sub>O<sub>2</sub> scavenging, H/L-TEAC, and H/L-ORAC assays moderate
to strong in vitro antioxidative activities