Additional file 2: of Cysteine boosters the evolutionary adaptation to CoCl2 mimicked hypoxia conditions, favouring carboplatin resistance in ovarian cancer

Table S1. ES2 and OVCAR3 cells resistance to hypoxia mimicked with CoCl2. Table S2. Metabolic evolution driven by hypoxia mimicked with CoCl2 provides stronger resistance to carboplatin. Table S3. ROS levels in ES2 (OCCC) and OVCAR3 (OSC) ancestral cells, cells selected under normoxia and under hypoxia mimicked with CoCl2. (DOCX 16 kb

Additional file 1: of Cysteine boosters the evolutionary adaptation to CoCl2 mimicked hypoxia conditions, favouring carboplatin resistance in ovarian cancer

Figure S1. ES2 and OVCAR3 cells resistance to hypoxia mimicked with CoCl2. Comparison of ES2 and OVCAR3 cells resistance to hypoxia mimicked with CoCl2 for 48 h of assay for A. ES2 and OVCAR3 cells in which values were normalized to the respective control, and B. ES2 and OVCAR3 cells with non-normalized to control values. N selected – cells selected under normoxia; H selected – cells selected under hypoxia mimicked with CoCl2. Results are shown as mean ± SD. Asterisks represent statistical significance between ES2 and OVCAR3 cells. *p < 0.05, **p < 0.01, ***p < 0.001 (Independent samples T tests). (TIFF 127 kb

Additional file 4: of Cysteine boosters the evolutionary adaptation to CoCl2 mimicked hypoxia conditions, favouring carboplatin resistance in ovarian cancer

Figure S3. ROS levels in ES2 (OCCC) and OVCAR3 (OSC) ancestral cells, cells selected under normoxia and under hypoxia mimicked with CoCl2. ROS levels in a drug-free environment for 48 h of assay for A. and B. ES2 cells and C. and D. OVCAR3 cells and ROS levels in the presence of Carboplatin for 48 h of assay for E. and F. ES2 cells and G. and H. OVCAR3. N selected – cells selected under normoxia; H selected – cells selected under hypoxia mimicked with CoCl2; N – Normoxia; NC – Normoxia supplemented with cysteine; H – Hypoxia mimicked with CoCl2; HC – Hypoxia mimicked with CoCl2 supplemented with cysteine. Results are shown as mean ± SD. Asterisks represent statistical significance compared to cells cultured under normoxia within each cell line. Cardinals represent statistical significance compared to cells cultured under hypoxia mimicked with CoCl2 within each cell line. *p < 0.05, **p < 0.01, ***p < 0.001 or #p < 0.05, ##p < 0.01, ###p < 0.001 (One-way ANOVA with post hoc Tukey tests). (TIFF 484 kb

Additional file 3: of Cysteine boosters the evolutionary adaptation to CoCl2 mimicked hypoxia conditions, favouring carboplatin resistance in ovarian cancer

Figure S2. Metabolic evolution driven by hypoxia mimicked with CoCl2 provides stronger resistance to carboplatin. Cell death levels (non-normalized values) in the presence of carboplatin for 48 h of assay for A. ES2 cells and B. OVCAR3 cells. N selected – cells selected under normoxia; H selected – cells selected under hypoxia mimicked with CoCl2; N – Normoxia; NC – Normoxia supplemented with cysteine; H – Hypoxia mimicked with CoCl2; HC – Hypoxia mimicked with CoCl2 supplemented with cysteine. Results are shown as mean ± SD. Asterisks represent statistical significance compared to cells cultured under normoxia within each cell line. Cardinals represent statistical significance compared to cells cultured under hypoxia within each cell line. *p < 0.05, **p < 0.01, ***p < 0.001 or #p < 0.05, ##p < 0.01, ###p < 0.001 (One-way ANOVA with post hoc Tukey tests). (TIFF 159 kb

Data_Sheet_1_Determinants of HIV late presentation among men who have sex with men in Portugal (2014–2019): who’s being left behind?.docx

IntroductionHIV late presentation (LP) remains excessive in Europe. We aimed to analyze the factors associated with late presentation in the MSM population newly diagnosed with HIV in Portugal between 2014 and 2019.MethodsWe included 391 newly HIV-1 diagnosed Men who have Sex with Men (MSM), from the BESTHOPE project, in 17 countrywide Portuguese hospitals. The data included clinical and socio-behavioral questionnaires and the viral genomic sequence obtained in the drug resistance test before starting antiretrovirals (ARVs). HIV-1 subtypes and epidemiological surveillance mutations were determined using different bioinformatics tools. Logistic regression was used to estimate the association between predictor variables and late presentation (LP).ResultsThe median age was 31 years, 51% had a current income between 501–1,000 euros, 28% were migrants. 21% had never been tested for HIV before diagnosis, with 42.3% of MSM presenting LP. 60% were infected with subtype B strains. In the multivariate regression, increased age at diagnosis, higher income, lower frequency of screening, STI ever diagnosed and higher viral load were associated with LP.ConclusionOur study suggests that specific subgroups of the MSM population, such older MSM, with higher income and lower HIV testing frequency, are not being targeted by community and clinical screening services. Overall, targeted public health measures should be strengthened toward these subgroups, through strengthened primary care testing, expanded access to PrEP, information and promotion of HIV self-testing and more inclusive and accessible health services.</p

Data_Sheet_1_Determinants of HIV late presentation among men who have sex with men in Portugal (2014–2019): who’s being left behind?.docx

IntroductionHIV late presentation (LP) remains excessive in Europe. We aimed to analyze the factors associated with late presentation in the MSM population newly diagnosed with HIV in Portugal between 2014 and 2019.MethodsWe included 391 newly HIV-1 diagnosed Men who have Sex with Men (MSM), from the BESTHOPE project, in 17 countrywide Portuguese hospitals. The data included clinical and socio-behavioral questionnaires and the viral genomic sequence obtained in the drug resistance test before starting antiretrovirals (ARVs). HIV-1 subtypes and epidemiological surveillance mutations were determined using different bioinformatics tools. Logistic regression was used to estimate the association between predictor variables and late presentation (LP).ResultsThe median age was 31 years, 51% had a current income between 501–1,000 euros, 28% were migrants. 21% had never been tested for HIV before diagnosis, with 42.3% of MSM presenting LP. 60% were infected with subtype B strains. In the multivariate regression, increased age at diagnosis, higher income, lower frequency of screening, STI ever diagnosed and higher viral load were associated with LP.ConclusionOur study suggests that specific subgroups of the MSM population, such older MSM, with higher income and lower HIV testing frequency, are not being targeted by community and clinical screening services. Overall, targeted public health measures should be strengthened toward these subgroups, through strengthened primary care testing, expanded access to PrEP, information and promotion of HIV self-testing and more inclusive and accessible health services.</p

Data_Sheet_1_Determinants of HIV late presentation among men who have sex with men in Portugal (2014–2019): who’s being left behind?.docx

IntroductionHIV late presentation (LP) remains excessive in Europe. We aimed to analyze the factors associated with late presentation in the MSM population newly diagnosed with HIV in Portugal between 2014 and 2019.MethodsWe included 391 newly HIV-1 diagnosed Men who have Sex with Men (MSM), from the BESTHOPE project, in 17 countrywide Portuguese hospitals. The data included clinical and socio-behavioral questionnaires and the viral genomic sequence obtained in the drug resistance test before starting antiretrovirals (ARVs). HIV-1 subtypes and epidemiological surveillance mutations were determined using different bioinformatics tools. Logistic regression was used to estimate the association between predictor variables and late presentation (LP).ResultsThe median age was 31 years, 51% had a current income between 501–1,000 euros, 28% were migrants. 21% had never been tested for HIV before diagnosis, with 42.3% of MSM presenting LP. 60% were infected with subtype B strains. In the multivariate regression, increased age at diagnosis, higher income, lower frequency of screening, STI ever diagnosed and higher viral load were associated with LP.ConclusionOur study suggests that specific subgroups of the MSM population, such older MSM, with higher income and lower HIV testing frequency, are not being targeted by community and clinical screening services. Overall, targeted public health measures should be strengthened toward these subgroups, through strengthened primary care testing, expanded access to PrEP, information and promotion of HIV self-testing and more inclusive and accessible health services.</p